抗阻訓練對老齡大鼠骨骼肌細胞凋亡和新生細胞核的補償研究
發(fā)布時間：2019-03-03 16:35
【摘要】：目的：抗阻訓練對骨骼肌衰老有積極的作用，本實驗采取新型尾部抗阻訓練方法研究骨骼肌細胞核凋亡和新生情況，為其內(nèi)部的機制研究提供基礎(chǔ)理論依據(jù)。同時在新型大鼠抗阻訓練的模型下研究其負荷強度水平對機體的影響狀況，為老年人健身負荷量提供理論參考。 方法：本實驗以18月齡SD健康大鼠32只為研究對象，隨機分為4組：安靜對照組（C）、小負荷抗阻訓練組（L）、中負荷抗阻訓練組（M）、大負荷抗阻訓練組（H）。分別對L組、M組、H組進行10周的新型尾部抗阻訓練干預(yù)，所有動物取材進行Brdu（5-溴脫氧尿嘧啶核苷）標記，10周末處死，，取腓腸肌，進行免疫組化實驗和生化指標的檢測，分析各組腓腸肌細胞核凋亡和新生情況，以及分析各組骨骼肌蛋白總量和MHC異形體mRNA表達狀況等。 結(jié)果： 1.10周末，與C組相比，各訓練組大鼠骨骼肌力量有顯著性增加，各訓練組腓腸肌蛋白總量也有顯著性的升高，L組和M組的α-actin mRNA表達有非常顯著性的升高. 2.10周末，與C組相比，L組和M組的MHCI mRNA、MHCIIa mRNA、MHCIIx mRNA顯著性升高，L組和M組以及H組的MHCIIb mRNA表達顯著性降低，H組的MHCI mRNA、MHCIIxmRNA表達顯著性降低。 3.10周末，與C組相比，M組和H組的細胞核凋亡百分比有顯著性的升高；與C組相比，L組和M組以及H組腓腸肌新生細胞核百分比有顯著性的升高。與C組相比，L組和M組的細胞更替百分比有顯著性的降低。 結(jié)論 1.10周尾部抗阻訓練能使老齡大鼠骨骼肌力量以及蛋白總量增多，且中負荷組效果最佳。 2.10周中、低強度的抗阻訓練使老齡大鼠骨骼肌快型和慢型MHC異行體mRNA表達量顯著性升高，表現(xiàn)為快型比例相對減少，慢型比例相對增多。 3.10周大、中負荷抗阻訓練能引起細胞核凋亡百分比顯著性增多，各個訓練組新生細胞核數(shù)目百分比也有顯著性的增多，小、中負荷的細胞核更替百分比顯著降低，從而延緩和對抗骨骼肌衰老。
[Abstract]:Aim: resistance training has a positive effect on skeletal muscle senescence. In this study, a new type of tail resistance training method was used to study the apoptosis and neogenesis of skeletal muscle nucleus, and provide the basic theoretical basis for the study of its internal mechanism. At the same time, under the model of resistance training of new type rats, the influence of load intensity on the body was studied, and the theoretical reference was provided for the body-building load of the elderly. Methods: thirty-two 18-month-old healthy SD rats were randomly divided into 4 groups: quiet control group (C), small load resistance training group (L), moderate load resistance training group (M), heavy load resistance training group (H). Group L, group M and group H were treated with new tail resistance training for 10 weeks. All animals were taken and labeled with Brdu (5-bromodeoxyuridine), then sacrificed at the end of 10 weeks, and gastrocnemius muscle was taken out, and the muscle of gastrocnemius was taken from the muscle of gastrocnemius. The apoptosis and neogenesis of gastrocnemius muscle nucleus and the total protein content of skeletal muscle and the expression of MHC heteroform mRNA in each group were analyzed by immunohistochemical assay and biochemical test. Results: 1. At the end of the 10th week, compared with group C, the skeletal muscle strength and the total protein of gastrocnemius muscle in each training group increased significantly. The expression of 偽-actin mRNA in L group and M group was significantly higher than that in group C. 2. At the end of 10 weeks, compared with C group, the expression of MHCI mRNA,MHCIIa mRNA,MHCIIxmRNA in L group and M group was significantly increased, the expression of MHCIIb mRNA in L group, M group and H group was significantly lower than that in H group, and the expression of MHCI mRNA,MHCIIxmRNA in H group was significantly lower than that in H group. 3. At the end of the week, compared with group C, the percentage of nucleus apoptosis in group M and group H was significantly higher than that in group C, and in group L, group M and group H, the percentage of new nuclei in gastrocnemius muscle was significantly higher than that in group C. Compared with C group, the percentage of cell turnover in L group and M group was significantly lower than that in C group. 10 weeks tail resistance training can increase the skeletal muscle strength and total protein of aged rats, and the middle load group has the best effect. 2. 2. In 10 weeks, low-intensity resistance training significantly increased the expression of fast and slow MHC heterozygous mRNA in skeletal muscle of aged rats, which showed that the proportion of fast type and slow type was relatively decreased, while the proportion of slow type was relatively increased. 3. At the age of 10 weeks, the percentage of nucleus apoptosis induced by resistance training in middle load increased significantly, and the percentage of new nuclei in each training group also increased significantly, and the percentage of nucleus replacement in middle load decreased significantly, while the percentage of nucleus replacement in middle load decreased significantly in each training group. Thus delaying and fighting skeletal muscle senescence.
【學位授予單位】：河北師范大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：G804.2
本文編號：2433891
[Abstract]:Aim: resistance training has a positive effect on skeletal muscle senescence. In this study, a new type of tail resistance training method was used to study the apoptosis and neogenesis of skeletal muscle nucleus, and provide the basic theoretical basis for the study of its internal mechanism. At the same time, under the model of resistance training of new type rats, the influence of load intensity on the body was studied, and the theoretical reference was provided for the body-building load of the elderly. Methods: thirty-two 18-month-old healthy SD rats were randomly divided into 4 groups: quiet control group (C), small load resistance training group (L), moderate load resistance training group (M), heavy load resistance training group (H). Group L, group M and group H were treated with new tail resistance training for 10 weeks. All animals were taken and labeled with Brdu (5-bromodeoxyuridine), then sacrificed at the end of 10 weeks, and gastrocnemius muscle was taken out, and the muscle of gastrocnemius was taken from the muscle of gastrocnemius. The apoptosis and neogenesis of gastrocnemius muscle nucleus and the total protein content of skeletal muscle and the expression of MHC heteroform mRNA in each group were analyzed by immunohistochemical assay and biochemical test. Results: 1. At the end of the 10th week, compared with group C, the skeletal muscle strength and the total protein of gastrocnemius muscle in each training group increased significantly. The expression of 偽-actin mRNA in L group and M group was significantly higher than that in group C. 2. At the end of 10 weeks, compared with C group, the expression of MHCI mRNA,MHCIIa mRNA,MHCIIxmRNA in L group and M group was significantly increased, the expression of MHCIIb mRNA in L group, M group and H group was significantly lower than that in H group, and the expression of MHCI mRNA,MHCIIxmRNA in H group was significantly lower than that in H group. 3. At the end of the week, compared with group C, the percentage of nucleus apoptosis in group M and group H was significantly higher than that in group C, and in group L, group M and group H, the percentage of new nuclei in gastrocnemius muscle was significantly higher than that in group C. Compared with C group, the percentage of cell turnover in L group and M group was significantly lower than that in C group. 10 weeks tail resistance training can increase the skeletal muscle strength and total protein of aged rats, and the middle load group has the best effect. 2. 2. In 10 weeks, low-intensity resistance training significantly increased the expression of fast and slow MHC heterozygous mRNA in skeletal muscle of aged rats, which showed that the proportion of fast type and slow type was relatively decreased, while the proportion of slow type was relatively increased. 3. At the age of 10 weeks, the percentage of nucleus apoptosis induced by resistance training in middle load increased significantly, and the percentage of new nuclei in each training group also increased significantly, and the percentage of nucleus replacement in middle load decreased significantly, while the percentage of nucleus replacement in middle load decreased significantly in each training group. Thus delaying and fighting skeletal muscle senescence.
【學位授予單位】：河北師范大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：G804.2
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