【摘要】：【目的】分析精制橘皮果膠(purified low molecular citrus pectin,PLMCP)的結(jié)構(gòu),為靶向性抗癌藥物載體的研究提供參考�！痉椒ā恳蚤倨すz為原料,制備精制橘皮果膠,采用改良間羥聯(lián)苯法測(cè)定其半乳糖醛酸含量,酸堿滴定法和氣相色譜法測(cè)定其酯化度,分子排阻色譜法測(cè)定其分子質(zhì)量,單糖乙酰化法測(cè)定其單糖含量,分析精制橘皮果膠的主要結(jié)構(gòu)�！窘Y(jié)果】精制橘皮果膠半乳糖醛酸含量為95.40%,酯化度為7.99%,分子質(zhì)量在13.69~24.58ku,重均分子質(zhì)量為17.94ku;單糖只檢出半乳糖醛酸,鼠李糖、阿拉伯糖、木糖、葡萄糖和半乳糖均未檢出,推測(cè)PLMCP為直鏈結(jié)構(gòu)同型聚半乳糖醛酸。【結(jié)論】精制橘皮果膠的結(jié)構(gòu)單一、分布均一、分子質(zhì)量穩(wěn)定,可作為新型靶向性抗癌藥物的載體。
[Abstract]:[objective] to analyze the structure of refined orange peel pectin (purified low molecular citrus) and to provide a reference for the study of targeted anticancer drug carrier. [methods] Pectin from orange peel was used as raw material to prepare refined orange peel pectin. Galacturonic acid content was determined by modified m-hydroxybiphenyl method, esterification degree was determined by acid-base titration and gas chromatography, molecular weight was determined by molecular exclusion chromatography, monosaccharide content was determined by monosaccharide acetylation method. The main structure of refined orange peel pectin was analyzed. [results] the content of galacturonic acid in refined orange peel pectin was 95.40, the degree of esterification was 7.99m, the molecular weight was 13.69m 24.58kuand the average molecular weight was 17.94ku.The monosaccharide only detected galacturonic acid, rhamnose, arabinose and xylose. Both glucose and galactose were not detected. It was speculated that PLMCP was a straight chain polygalacturonic acid. [conclusion] the purified orange peel pectin has a single structure, uniform distribution, stable molecular weight, and can be used as a carrier of new targeted anticancer drugs.
【作者單位】： 四川師范大學(xué)生命科學(xué)學(xué)院;重慶萊美藥業(yè)股份有限公司;
【基金】：國(guó)家“十二五”重大專項(xiàng)(2011ZX09102-001)
【分類號(hào)】：R914
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本文編號(hào)：2125120