發(fā)布時(shí)間：2018-04-15 18:32

  本文選題：快速高分離液相色譜-電噴霧質(zhì)譜 + 蛇酒��； 參考：《中華中醫(yī)藥雜志》2017年12期

【摘要】：目的:建立同時(shí)測(cè)定蛇酒中醋酸潑尼松、醋酸氫化可的松、醋酸地塞米松、布地奈德和甲潑尼龍的快速高分離液相色譜-電噴霧質(zhì)譜聯(lián)用方法。方法:采用Agilent Poroshell 120 EC-C18色譜柱(100mm×3mm,2.7μm),乙腈(含0.1%甲酸)-0.1%甲酸水溶液為流動(dòng)相進(jìn)行梯度洗脫,流速0.3m L/min;采用電噴霧離子源,以正離子、多反應(yīng)監(jiān)測(cè)掃描模式對(duì)蛇酒中的5種糖皮質(zhì)激素進(jìn)行定性定量檢測(cè),并對(duì)其裂解方式進(jìn)行解析。結(jié)果:醋酸潑尼松、醋酸氫化可的松、醋酸地塞米松、布地奈德和甲潑尼龍線性關(guān)系良好,R2均0.99,8種蛇酒中檢測(cè)到糖皮質(zhì)激素。結(jié)論:本研究建立的快速高分離液相色譜-電噴霧質(zhì)譜聯(lián)用法方便快捷、靈敏度好、準(zhǔn)確度高,適用于蛇酒中非法添加成分的定性定量檢測(cè)。
[Abstract]:Objective: to establish a method for simultaneous determination of prednisone acetate, hydrocortisone acetate, dexamethasone acetate, budesonide and methylprednisolone in snake wine.Methods: Agilent Poroshell 120 EC-C18 column (100mm 脳 3mm) was used for gradient elution with acetonitrile (0.1% formate-0.1% formic acid aqueous solution as mobile phase) at a flow rate of 0.3m L / min.Five kinds of glucocorticoids in snake wine were detected qualitatively and quantitatively by multi-reaction monitoring scanning mode, and their cleavage modes were analyzed.Results: glucocorticoids were detected in 8 snake wines, prednisone acetate, hydrocortisone acetate, dexamethasone acetate, budesonide and methylprednisolone.Conclusion: the rapid and high separation liquid chromatography-electrospray mass spectrometry method is convenient, rapid, sensitive and accurate. It is suitable for the qualitative and quantitative determination of illegally added components in snake wine.
【作者單位】： 浙江中醫(yī)藥大學(xué);
【基金】：浙江省自然科學(xué)基金項(xiàng)目(N o.L Y14H270014) 浙江省公益性技術(shù)應(yīng)用研究計(jì)劃項(xiàng)目(No.2016C33086) 浙江中醫(yī)藥大學(xué)校級(jí)科研基金項(xiàng)目(No.711200F017);浙江中醫(yī)藥大學(xué)基礎(chǔ)醫(yī)學(xué)院“中醫(yī)證的本質(zhì)研究”創(chuàng)新團(tuán)隊(duì)(No.JCIT2016-3)~~
【分類(lèi)號(hào)】：O657.63;R284.1

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本文編號(hào)：1755284