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  本文選題：熒光探針 + 次氯酸根��； 參考：《上海師范大學》2017年碩士論文

【摘要】：本文主要分為兩個部分:以氟硼二吡咯(BODIPY)為熒光團、肼基為測試基團,基于自由基反應機理,用于檢測次氯酸根離子的高靈敏性、高選擇性熒光探針;以無機介孔硅納米材料(MCM-41)為基底材料,通過表面修飾,實現(xiàn)pH與光照雙重控制的藥物傳輸體系。⑴第一部分。由于人體內(nèi)次氯酸根離子的濃度與人體健康息息相關,故發(fā)展檢測次氯酸根離子的高選擇性、高靈敏性熒光探針引起了人們的廣泛關注。本文合成了一種以氟硼二吡咯(BODIPY)為熒光團、以肼基作為次氯酸根檢測基團,用于檢測次氯酸根離子的新型熒光探針,并對其性質(zhì)做了探討。該探針與次氯酸鈉的反應在磷酸鹽緩沖液中一分鐘之內(nèi)即可完成,反應完成后體系的熒光強度急劇增加。同時,該探針表現(xiàn)出對次氯酸根離子的高選擇性和高靈敏度。高效液相色譜、核磁共振氫譜和質(zhì)譜的數(shù)據(jù)分析表明,次氯酸根與該探針的反應極有可能是通過自由基氧化機理完成的。⑵第二部分。目前,藥物控釋體系在醫(yī)藥研發(fā)以及實際應用等方面?zhèn)涫苋藗兊那嗖A。在本文中,我們制備了一種基于介孔納米硅材料MCM-41,pH和光照雙重控制的藥物傳輸體系。該體系分別將氨基硅源與偶氮苯衍生物修飾于MCM-41表面,其中通過對氨基硅源進行修飾并與乙二胺-β-環(huán)糊精形成亞胺鍵來作為pH控制部件;偶氮苯衍生物與β-環(huán)糊精形成分子復合體作為光照控制部件。兩個控制部件通過一個β-環(huán)糊精分子聯(lián)結為一體。光照控制部件經(jīng)紫外線照射(365nm)會被打開;pH控制部件在酸性環(huán)境中(磷酸鹽緩沖液,pH=5.0)會被打開。然而此時材料孔道周圍的β-環(huán)糊精并未離去,其將繼續(xù)阻止載體分子釋放。只有在酸性環(huán)境中(磷酸鹽緩沖液,pH=5.0)且有紫外光(365 nm)照射時,pH控制部件和光照控制部件會同時打開,β-環(huán)糊精離開材料孔道周圍,載體分子大量釋放出來。實驗結果表明,通過控制納米門的開/關,能夠控制載體部分地釋放。如果將材料的每個孔道看做一所房子,β-環(huán)糊精看做房子上的一道門,pH控制部件和光照控制部件看做門上的兩把鎖,那么,只有在同時擁有兩把鑰匙時才能將這道門打開,將房子里的貨物搬出來。如此設計是為了更加精確地控制載體從材料中釋放出來,降低藥物在到達病變部位之前對正常組織所產(chǎn)生的毒副作用。
[Abstract]:This paper is mainly divided into two parts: fluoroborboron dipyrrolidine (BODIPY) as fluorescence group, hydrazine group as test group, based on the mechanism of free radical reaction, used for the detection of hypochlorite ion high sensitivity, high selectivity fluorescence probe;Using inorganic mesoporous silicon nanomaterials (MCM-41) as substrate, the drug transport system with double pH and illumination was realized by surface modification.Because the concentration of hypochlorite ion in human body is closely related to human health, the development of the detection of hypochlorite ion high selectivity, high sensitivity fluorescent probe has attracted people's attention.In this paper, a new fluorescent probe for the detection of hypochlorite ions using fluoroborate dipyrrolidine (BODIPY) as fluorescence group and hydrazine as detection group was synthesized, and its properties were discussed.The reaction between the probe and sodium hypochlorite was completed within one minute in the phosphate buffer, and the fluorescence intensity of the system increased sharply after the reaction was completed.At the same time, the probe showed high selectivity and high sensitivity to hypochlorite ions.High performance liquid chromatography (HPLC), nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS) showed that the reaction of hypochlorite with the probe was most likely due to the second part of the radical oxidation mechanism.At present, drug-controlled release system is very popular in pharmaceutical research and development as well as practical application.In this paper, we have prepared a drug transport system based on the double control of pH and illumination of mesoporous nanocrystalline silicon (MCM-41).In this system, the amino silicon source and azobenzene derivatives were modified on the surface of MCM-41, in which the amino silicon source was modified and the imine bond was formed with ethylenediamine- 尾 -cyclodextrin as the pH control component.Azobenzene derivatives and 尾-cyclodextrin form molecular complexes as light control parts.Two control components are bound together by a 尾-cyclodextrin molecule.The light control unit was exposed to ultraviolet light (365 nm) and the pH control unit was opened in acidic environment (phosphate buffer pH 5.0).At this time, however, the 尾-cyclodextrin around the pore does not leave, and it will continue to block the release of the carrier molecule.Only in acidic environment (phosphate buffer pH 5.0 and UV irradiation 365 nm) will the pH control component and the light control component open simultaneously, and 尾 -cyclodextrin leave the pore of the material and the carrier molecules will be released in large quantities.The experimental results show that the partial release of the support can be controlled by controlling the opening / closing of the nanogates.If you look at each hole in the material as a house, 尾 -cyclodextrin as a door pH control component and a light control component as two locks on the door, then you can only open the door if you have two keys at the same time.Move the goods out of the house.This design is designed to more precisely control the release of the carrier from the material to reduce the toxic side effects of the drug on normal tissue before reaching the diseased site.
【學位授予單位】：上海師范大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：O657.3;TQ460.1

【參考文獻】

相關期刊論文 前1條

1 SUTHIWANGCHAROEN Nisaraporn;;Tuning the optical properties of BODIPY dye through Cu(Ⅰ) catalyzed azide-alkyne cycloaddition(CuAAC) reaction[J];Science China(Chemistry);2012年01期

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本文編號：1755232