發(fā)布時間：2018-04-15 19:23

  本文選題：分子印跡固相萃取 + 高效液相色譜�。� 參考：《中國食品學(xué)報》2017年08期

【摘要】：目的:建立分子印跡固相萃取與高效液相色譜聯(lián)用方法,檢測蜂王漿及其制品中蜂王酸的含量。方法:采用分子印跡本體聚合法,經(jīng)一系列優(yōu)化,得到對蜂王酸具有特異識別能力和高吸附容量的分子印跡聚合物。將其作為固相萃取柱的填充材料,對蜂王漿樣品中的蜂王酸進(jìn)行分離和純化,采用高效液相色譜檢測。結(jié)果:被測樣品用分子印跡固相萃取柱處理后的加標(biāo)回收率為92.02%~103.28%,檢出限(S/N=3)為0.94μg/L,線性范圍10~1 000μg/L(R20.99),相對標(biāo)準(zhǔn)偏差(RSD)小于8.91%。結(jié)論:所建方法具有準(zhǔn)確性和實(shí)用性,可用于蜂王漿及其制品中蜂王酸的分離純化和檢測。
[Abstract]:Objective: to establish a method for the determination of bee royal acid in royal jelly and its products by molecularly imprinted solid phase extraction and high performance liquid chromatography.Methods: the molecularly imprinted polymers with special recognition ability and high adsorption capacity were obtained by a series of optimization methods by molecular imprinting bulk polymerization.It was used as a filling material for solid phase extraction column to separate and purify bee royal acid from royal jelly samples and was detected by high performance liquid chromatography (HPLC).Results: the recoveries of the samples treated with molecularly imprinted solid-phase extraction column were 92.022 and 103.28, the detection limit was 0.94 渭 g / L, the linear range was 101000 渭 g / L, and the relative standard deviation (RSDs) was less than 8.91%.Conclusion: the method is accurate and practical, and can be used for the separation, purification and determination of bee royal acid in royal jelly and its products.
【作者單位】： 食品營養(yǎng)與安全教育部重點(diǎn)實(shí)驗(yàn)室天津科技大學(xué)食品工程與生物技術(shù)學(xué)院;
【基金】：國家重點(diǎn)研發(fā)計劃項(xiàng)目(2016YFD0400401)
【分類號】：O657.72;S896.3

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