發(fā)布時間：2018-08-17 15:47

【摘要】：目的評估syndecan-1(Sdc1)對大鼠心肌成纖維細(xì)胞生物學(xué)行為的影響,為心肌梗死(MI)后心室重構(gòu)的防治提供依據(jù)。方法體外分離培養(yǎng)SD大鼠乳鼠心肌成纖維細(xì)胞,分別轉(zhuǎn)染Sdc1 siRNA質(zhì)粒和Sdc1高表達(dá)質(zhì)粒,并設(shè)立空白對照組、無關(guān)序列和空載質(zhì)粒作為陰性對照。采用流式細(xì)胞術(shù)檢測細(xì)胞增殖,CCK-8法檢測細(xì)胞活力,酶聯(lián)免疫法(ELISA)檢測膠原(羥脯氨酸)分泌能力及Transwell細(xì)胞遷移實(shí)驗(yàn)檢測細(xì)胞遷移能力。結(jié)果Sdc1 siRNA組心肌成纖維細(xì)胞S期比例、增殖指數(shù)、細(xì)胞活力、膠原(羥脯氨酸)分泌量和遷移細(xì)胞數(shù)分別為(8.36±0.23)%、(13.2±0.5)%、(1.72±0.12)、(1162.3±60.4)pg/mL和170個/孔,明顯高于空白對照和陰性對照組(P0.01);Sdc1高表達(dá)質(zhì)粒組心肌成纖維細(xì)胞S期比例、增殖指數(shù)、細(xì)胞活力、膠原(羥脯氨酸)分泌量和遷移細(xì)胞數(shù)分別為(6.48±0.22)%、(10.8±0.6)%、(1.30±0.11)、(346.8±52.1)pg/mL和50個/孔,明顯低于空白對照和空載質(zhì)粒組(P0.01)。結(jié)論 Sdc1能夠抑制心肌成纖維細(xì)胞的增殖、活力、遷移和分泌能力。
[Abstract]:Objective to evaluate the effects of syndecan-1 (Sdc1) on the biological behavior of cardiac fibroblasts in rats and to provide evidence for the prevention and treatment of ventricular remodeling after myocardial infarction (MI). Methods SD rat myocardial fibroblasts were isolated and cultured in vitro and transfected with Sdc1 siRNA plasmid and Sdc1 high expression plasmid respectively. The blank control group was set up and the unrelated sequence and empty plasmid were used as negative control. The cell viability was detected by flow cytometry (FCM) and CCK-8 assay. The activity of collagen (hydroxyproline) was detected by enzyme-linked immunosorbent assay (ELISA) and the migration ability of Transwell cells was detected by Transwell cell migration assay. Results in Sdc1 siRNA group, the ratio of S phase, proliferation index, cell viability, secretion of collagen (hydroxyproline) and the number of migrating cells were (8.36 鹵0.23), (13.2 鹵0.5), (1.72 鹵0.12), () 1162.3 鹵60.4 pg/mL and 170 / well, respectively. The ratio of S phase, proliferation index, cell viability, collagen (hydroxyproline) secretion and the number of migrating cells were (6.48 鹵0.22), (10.8 鹵0.60), (1.30 鹵0.11), (鹵52.1) pg/mL and (50 / well), respectively. It was significantly lower than the blank control group and the blank plasmid group (P0.01). Conclusion Sdc1 can inhibit the proliferation, activity, migration and secretion of myocardial fibroblasts.
【作者單位】： 中山大學(xué)孫逸仙紀(jì)念醫(yī)院;
【基金】：廣東省科技計劃項(xiàng)目(2013B021800096)
【分類號】：R542.22
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本文編號：2188128