本文選題：動(dòng)脈粥樣硬化　切入點(diǎn)：miR-　出處：《廣東醫(yī)學(xué)》2017年17期

【摘要】：目的探討動(dòng)脈粥樣硬化(AS)患者外周血miR-152及其啟動(dòng)子區(qū)DNA甲基化水平改變,并分析其與血脂水平的相關(guān)性。方法選擇經(jīng)頸動(dòng)脈多普勒超聲檢查確診為AS患者40例(AS組),并選擇同期健康體檢者40例作為對(duì)照(對(duì)照組)。生化分析儀檢測(cè)兩組患者血脂水平;實(shí)時(shí)熒光定量PCR(RT-qPCR)檢測(cè)兩組患者外周血miR-152的表達(dá)水平;巢式降落式甲基化特異性PCR(nt-MSP)檢測(cè)兩組患者外周血miR-152 DNA甲基化水平;采用SPSS 19.0統(tǒng)計(jì)軟件對(duì)miR-152的表達(dá)水平與血脂水平進(jìn)行相關(guān)性分析。結(jié)果 AS組患者血清總膽固醇(TC)、三酰甘油(TG)和低密度脂蛋白(LDL)顯著高于對(duì)照組(均P0.01),而高密度脂蛋白(HDL)則顯著低于對(duì)照組(P0.01);AS組患者外周血miR-152的表達(dá)水平較對(duì)照組顯著降低(P0.01),而miR-152啟動(dòng)子區(qū)DNA甲基化水平較對(duì)照組顯著升高(P0.01);相關(guān)性分析結(jié)果顯示,miR-152的表達(dá)水平與TC、TG和LDL水平呈負(fù)相關(guān),相關(guān)系數(shù)分別為-0.71、-0.69和-0.58;與HDL呈正相關(guān),相關(guān)系數(shù)為0.61。結(jié)論AS患者外周血miR-152啟動(dòng)子區(qū)DNA甲基化程度升高使其表達(dá)水平降低,可能影響血液中血脂含量并最終導(dǎo)致AS的發(fā)生、發(fā)展。
[Abstract]:Objective to investigate the changes of DNA methylation level in peripheral blood miR-152 and its promoter in patients with atherosclerotic atherosclerosis (ASA). Methods 40 patients with as diagnosed by carotid Doppler ultrasound were selected as control group and 40 healthy persons were selected as control group (control group). Biochemical analyzer was used to detect blood lipid level in both groups. The expression of miR-152 in peripheral blood of the two groups was detected by real-time fluorescence quantitative PCR RT-PCR, and the miR-152 DNA methylation level in peripheral blood of the two groups was detected by nested landing methylation specific PCRnt-MSPs. Results the levels of serum total cholesterol, triglyceride and low density lipoprotein in as group were significantly higher than those in control group (all P 0.01, and high density lipid). The expression level of miR-152 in peripheral blood of patients with P0.01as was significantly lower than that of the control group, while the DNA methylation level of miR-152 promoter was significantly higher than that of the control group. The correlation analysis showed that the expression level of DNA in the miR-152 promoter region was significantly higher than that in the control group, and the correlation analysis showed that the expression level of Mir 152 was significantly higher than that of the control group. TG and LDL levels were negatively correlated. The correlation coefficients were -0.71 鹵-0.69 and -0.58, respectively, which were positively correlated with HDL, and the correlation coefficient was 0.61.Conclusion the increased degree of DNA methylation in peripheral blood miR-152 promoter region may decrease the expression level of as, which may affect the blood lipid content and eventually lead to the occurrence and development of as.
【作者單位】： 寧夏醫(yī)科大學(xué)附屬自治區(qū)人民醫(yī)院心血管內(nèi)科;寧夏醫(yī)科大學(xué)總醫(yī)院放療科;
【基金】：寧夏自然科學(xué)基金項(xiàng)目(編號(hào):NZ16206)
【分類(lèi)號(hào)】：R543.5
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本文編號(hào)：1699325