體重是家禽產(chǎn)業(yè)最重要的性狀之一。生長受多種因素調(diào)控,如基因、激素、營養(yǎng)和環(huán)境。GH基因具有過重生理學(xué)功能,包括促進(jìn)肌肉生長、調(diào)控脂肪的生長、骨骼發(fā)育和代謝。胰島素樣生長因子IGF-Ⅰ和IGF-Ⅱ基因?qū)τ诳傮w體重和肌肉生長至關(guān)重要,它們被認(rèn)為是與胰島素具有相似結(jié)構(gòu)的促有絲分裂多肽。京海黃雞是以當(dāng)?shù)氐胤近S雞資源為育種素材,根據(jù)傳統(tǒng)遺傳育種理論,采用常規(guī)育種和分子標(biāo)記輔助選擇,經(jīng)7個世代培育而成的小型優(yōu)質(zhì)肉雞新品種,京海黃雞具有耐粗飼和抗逆性強(qiáng)的特點(diǎn)。由于之前研究檢測到了許多與京海黃雞生長和繁殖性狀相關(guān)的SNPs位點(diǎn),因此其被認(rèn)為是具有發(fā)展前景的肉雞新品種。在本研究中,我們檢測了 GH、IGF-Ⅰ和IGF-Ⅱ基因中可能影響京海黃雞生長和繁殖性狀的突變位點(diǎn),并分析了這些位點(diǎn)之間的相互作用。所有的試驗(yàn)雞均孵化于同一天,籠養(yǎng),自由采食。記錄生長和繁殖數(shù)據(jù),并收集母雞血樣。由公雞和母雞的目標(biāo)器官中提取總RNA以研究基因表達(dá)模式,所有樣本均隨機(jī)選自飼養(yǎng)于江蘇省京海禽業(yè)集團(tuán)有限公司的同一批次的京海黃雞。使用PCR、SSCP和qPCR技術(shù)分析嗎目的基因的多態(tài)性、表達(dá)譜和表達(dá)規(guī)律,并分析它們與生長和繁殖性狀的...

【文章頁數(shù)】：139 頁

【學(xué)位級別】：博士

【文章目錄】：
DEDICATION
ABSTRACT
摘要
LIST OF ABBREVIATIONS & SYMBOLS
CHAPTER1: LITERATURE REVIEW
    1.1 INTRODUCTION
    1.2 THE CHICKEN GROWTH HORMONE GENE (GH)
        1.2.1 The GH, Somatotropic and Thyrotropic Axes
    1.3 THE INSULIN-LIKE GROWTH FACTOR-Ⅰ GENE (IGF-Ⅰ)
        1.3.1 The Insulin-like growth Factor-Ⅰ gene (IGF-Ⅰ) axis
    1.4 THE INSULIN-LIKE GROWTH FACTOR-Ⅱ GENE (IGF-Ⅱ)
        1.4.1 The Insulin-like growth Factor-Ⅱ gene (IGF-Ⅱ) axis
    1.5 THE GROWTH HORMONE (GH) AND BONE
        1.5.1 Effects of GH and IGF-Ⅰ on Longitudinal Bone Growth
        1.5.2 The physiological role of GH and IGF-Ⅰ for bone growth
    REFERENCE
CHAPTER 2: ASSOCIATION ANALYSIS WITH GROWTH AND REPRODUCTIVE TRAITS AND THE EXPRESSION PROFILES OF THE GH GENE IN JINGHAI YELLOW CHICKENS
    2.1 INTRODUCTION
    2.2 MATERIAL AND METHODs
        2.2.1 Population and samples collection
        2.2.2 DNA extraction and genotyping
        2.2.3 Primer design and PCR amplification
        2.2.4 Single-strand conformation polymorphism (SSCP) and sequencing
        2.2.5 Expression profiles analysis
        2.2.6 Statistical analysis
    2.3 RESULTS
        2.3.1 Polymorphisms of GH and genetic variation analysis
        2.3.2 Allele and genotype frequency of SNP in GH gene
        2.3.3 Association analysis with growth traits in CH gene
        2.3.4 Association analysis with reproductive traits in CH gene
        2.3.5 Association analysis of interaction effect for growth traits in CH gene
        2.3.6 Association analysis of interaction effect for reproductive traits
        2.3.7 Expression profile of GH gene in male and female Jinghai yellow chickens
        2.3.8 Expression regularity of GH gene in male and female Jinghai yellow chickens
    2.4 DISCUSSION
    REFERENCE
CHAPTER 3: ASSOCIATION ANALYSIS WITH GROWTH AND REPRODUCTIVE TRAITS AND THE EXPRESSION PROFILES OF THE IGF-Ⅰ GENE IN JINGHAI YELLOW CHICKENS
    3.1 INTRODUCTION
    3.2 MATERIAL AND METHODS
        3.2.1 Population and samples collection
        3.2.2 DNA extraction and genotyping
        3.2.3 Primer design and PCR amplification
        3.2.4 Single-strand conformation polymorphism (SSCP) and sequencing
        3.2.5 Expression profiles analysis
        3.2.6 Statistical analysis
    3.3 RESULTs
        3.3.1 Polymorphisms of IGF-Ⅰ and genetic variation analysis
        3.3.2 Allele and genotype frequencies of SNP in IGF-Ⅰ gene
        3.3.3 Association analysis with growth traits
        3.3.4 Association analysis with reproductive traits
        3.3.5 Expression profile of IGF-Ⅰ gene in male and female Jinghai yellow chickens
        3.3.6 Expression regularity of IGF-Ⅰ gene in male and female Jinghai yellow chickens
    3.4 DISCUSSION
    REFERENCE
CHAPTER 4: ASSOCIATION ANALYSIS WITH GROWTH AND REPRODUCTIVE TRAITS AND THE EXPRESSION PROFILES OF THE IGF-Ⅱ GENE IN JINGHAI YELLOW CHICKENS
    4.1 INTRODUCTION
    4.2 MATERIAL AND METHODS
        4.2.1 Population and samples collection
        4.2.2 DNA extraction and genotyping
        4.2.3 Primer design and PCR amplification
        4.2.4 Single-strand conformation polymorphism (SSCP) and sequencing
        4.2.5 Expression profiles analysis
        4.2.6 Statistical analysis
    4.3 RESULTS
        4.3.1 Polymorphisms of IGF-Ⅱ and genetic variation analysis
        4.3.2 Allele and genotype frequency of SNP in IGF-Ⅱ gene
        4.3.3 Association analysis with growth traits
        4.3.4 Association analysis with reproductive traits
        4.3.5 Association analysis of interaction effect for growth traits
        4.3.6 Association analysis of interaction effect for Reproductive traits
        4.3.7 Expression profiles of IGF-Ⅱ gene in male and female Jinghai yellow chickens
        4.3.8 Expression regularity of IGF-Ⅱ gene in male and female Jinghai yellow chickens
    4.4 DISCUSSION
    REFERENCE
CHAPTER 5: ASSOCIATION ANALYSIS OF INTERACTION EFFECT FOR GROWTH AND REPRODUCTIVE TRAITS AMONG THE DETECTED MUTATIONS IN THE GH, IGF-Ⅰ AND IGF-Ⅱ GENES IN JINGHAI YELLOW CHICKENS
    5.1 INTRODUCTION
    5.2 MATERIAL AND METHODS
        5.2.1 Statistical analysis
    5.3 RESULTS
        5.3.1 Association analysis of interaction effect for growth traits between detected mutations in the GH and IGF-Ⅰ genes
        5.3.2 Association analysis of interaction effect for growth traits between detected mutations in the GH and IGF-Ⅱ genes
        5.3.3 Association analysis of interaction effect for growth traits between detected mutations in the IGF-Ⅰ and IGF-Ⅱ genes
        5.3.4 Association analysis of interaction effect for reproductive traits between detected mutations in the GH and IGF-Ⅰ genes
        5.3.5 Association analysis of interaction effect for reproductive traits between detected mutations in the GH and IGF-Ⅱ genes
        5.3.6 Association analysis of interaction effect for reproductive traits between detected mutations in the IGF-Ⅰ and IGF-Ⅱ genes
        5.3.7 The comparison of expression profile between the GH and IGF-Ⅰ genes in male and female Jinghai yellow chickens
        5.3.8 The comparison of expression profile between the GH and IGF-Ⅱ genes in maleand female Jinghai yellow chickens
        5.3.9 The comparison of expression profile between the IGF-Ⅰand IGF-Ⅱ genes in maleand female Jinghai yellow chickens
        5.3.10 The comparison of expression profile between the GH and IGF-Ⅰ genes in Breastand Leg muscle for both male and female Jinghai yellow chickens
        5.3.11 The comparison of expression profile between the GH and IGF-Ⅱ genes in Breastand Leg muscle for both male and female Jinghai yellow chickens
        5.3.12 The comparison of expression profile between the IGF-Ⅰ and IGF-Ⅱ genes inBreast and Leg muscle for both male and female Jinghai yellow chickens
    5.4 DISGUSSION
    REFERENCE
CONCLUSION
LIST OF PUBLICATIONS



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