發(fā)布時(shí)間：2018-03-10 17:44

  本文選題：口蹄疫　切入點(diǎn)：抗體　出處：《吉林大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：為摸清天津地區(qū)豬口蹄疫免疫抗體水平，在2013年1月至12月期間，采集豬場(chǎng)的7639份血清，采用液相阻斷ELISA試驗(yàn)，對(duì)免疫抗體進(jìn)行檢測(cè)，，結(jié)果為合格6491份，合格率為84.97%。通過對(duì)不同規(guī)模豬場(chǎng)分析得出合格率依次為：種豬場(chǎng)、規(guī)模場(chǎng)、散養(yǎng)戶；近郊五個(gè)區(qū)縣免疫抗體水平高于遠(yuǎn)郊五個(gè)養(yǎng)殖大縣；從月份來看5月和11月是全年免疫抗體水平較高月份；在豬群分日齡比較中得出,口蹄疫抗體陽性合格率從高到低是：經(jīng)產(chǎn)母豬＞育肥豬＞仔豬＞保育豬。 通過非結(jié)構(gòu)蛋白抗體3ABC-I-ELISA試驗(yàn)對(duì)采集的2176份豬血清樣品進(jìn)行檢測(cè)，以掌握滅活疫苗免疫效果。結(jié)果檢出非結(jié)構(gòu)蛋白3ABC抗體陽性樣品44份，分析認(rèn)為天津不同地區(qū)、豬群及養(yǎng)殖方式中，均存在曾感染口蹄疫動(dòng)物，表明目前天津地區(qū)口蹄疫防控存在威脅，因此制定了以免疫預(yù)防、日常管理和應(yīng)急處置為一體的綜合防控措施，防止區(qū)域性口蹄疫疫情發(fā)生。
[Abstract]:In order to find out the antibody level of swine foot-and-mouth disease (FMD) in Tianjin area, from January 2013 to December, 7639 sera from pig farm were collected and detected by liquid phase blocking ELISA test. The results showed that the antibody was up to 6 491. The qualified rate was 84.97. Through the analysis of pig farms of different sizes, the qualified rates were obtained as follows: breeding farm, scale field, and scattered households, and the level of immune antibody in five districts in the suburbs was higher than that in the five major farming counties in the far suburbs. In May and November, the immune antibody level was higher in the whole year, and it was found that the qualified rate of FMD antibody from high to low was: sows > fattening pigs > piglets > care pigs. Non-structural protein antibody 3ABC-I-ELISA was used to detect 2176 pig serum samples in order to control the immune effect of inactivated vaccine. Results 44 non-structural protein 3ABC antibody positive samples were detected and analyzed in different areas of Tianjin. Pigs and breeding methods have been infected with foot-and-mouth disease animals, indicating that there is a threat to the prevention and control of foot-and-mouth disease in Tianjin. Therefore, comprehensive prevention and control measures have been formulated to integrate immunization prevention, daily management and emergency treatment. Prevent the outbreak of regional foot-and-mouth disease.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S858.28

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