本文關(guān)鍵詞：銀杏葉提取物對(duì)野百合堿所致肺動(dòng)脈高壓保護(hù)作用的實(shí)驗(yàn)研究，，由筆耕文化傳播整理發(fā)布。

銀杏葉提取物對(duì)野百合堿所致肺動(dòng)脈高壓保護(hù)作用的實(shí)驗(yàn)研究

Protective effect of ginkgo biloba extract on monocrotaline-induced pulmonary hypertension

[1] [2] [3] [4] [5] [6] [7]

Shen Tao, Wu Xu, Zhang Zhenzhong, Gu Chengwei, Li Jun, Wu Xia, Gao Dongyun （Department of Thoracic and Cardiovascular Surgery, Nanfang Hospital, Southern Medical University, G

南方醫(yī)科大學(xué)附屬南方醫(yī)院胸心血管外科,廣東廣州510515

文章摘要：目的：探討銀杏葉提取物對(duì)野百合堿所致肺動(dòng)脈高壓（PAH）的早期保護(hù)作用。方法：30只雄性 SD大鼠隨機(jī)分為3組：對(duì)照組、PAH 模型組和治療組,每組10只。PAH 模型組和治療組大鼠采用脊背部皮下注射1%野百合堿60 mg/kg的方法復(fù)制PAH 模型,注射后第2天開(kāi)始每日予2 ml 0.9%氯化鈉注射液（PAH 模型組）或60 mg/kg銀杏葉提取物（治療組）灌胃；對(duì)照組脊背部皮下注射等量溶劑,注射后第2天開(kāi)始每日予2 ml 0.9%氯化鈉注射液灌胃。第22天采用頸外靜脈右心導(dǎo)管法測(cè)定平均肺動(dòng)脈壓（mPAP）、右心室收縮壓（RVSP）,測(cè)定右心室、左心室、室間隔的重量,計(jì)算右心室肥厚指數(shù)（RVHI）；采用 HE染色觀察肺細(xì)小動(dòng)脈組織結(jié)構(gòu)情況,并計(jì)算直徑50~150μm的肺小動(dòng)脈血管管壁厚度占血管厚度百分比（WA%）和血管管壁面積與血管面積比值（WV%）；采用免疫組化方法觀察內(nèi)皮型一氧化氮合成酶（eNOS）及內(nèi)皮素-1（ET-1）在大鼠肺組織中的表達(dá)情況。結(jié)果：PAH 模型組、治療組的 mPAP、RVSP、RVHI、WA%、WV%與對(duì)照組比較均明顯升高（P〈0.05）,治療組的 mPAP、RVSP、WA%、WV%較 PAH 模型組均降低（P〈0.05）,而治療組與 PAH 模型組的RVHI差異無(wú)顯著性。免疫組化結(jié)果顯示,3組 eNOS表達(dá),對(duì)照組〉治療組〉PAH 模型組；3組 ET-1的表達(dá), PAH 模型組〉治療組〉對(duì)照組。結(jié)論：銀杏葉提取物通過(guò)抑制 ET-1的合成、減輕內(nèi)皮細(xì)胞損傷、維持 eNOS的表達(dá),達(dá)到減緩PAH 進(jìn)展的作用。

Abstr：Objective：To investigate the protective effect of ginkgo biloba extract （GBE）on monocrotaline-in-duced pulmonary hypertension in early stage.Methods：Thirty male SD rats were randomly divided into three groups ：control group,pulmonary hypertension model group （model group）,ginkgo biloba extract treatment group （treatment group,n=10）. The pulmonary hypertension model was reproduced by subcutaneous injection of monocrotaline （60 mg/kg）on the back,and then,2 ml normal saline or 60 mg/kg GBE was given by gavage from the second day in model group or treatment group. In control group,the same volume of vehicle was subcutane-ously inj ected instead of monocrotaline,and 2 ml normal saline was given by gavage from the second day. Mean pulmonary arterial pressure （mPAP）and right ventricular systolic pressure （RVSP）were measured through cath-eterization into the right ventricle via the right jugular vein on the 22nd day. The right ventricular,left ventricle, and interventricular septum were weighed to calculate the right ventricular hypertrophic index （RVHI）.The structure of pulmonary arterioles was observed with HE staining. The ratio of wall thickness/diameter of arteri-oles （WA%）,and the ratio of vascular wall area/vascular cross-sectional area （WV%）of pulmonary arterioles with diameter of 50-150μm were calculated. The expression of endothelial nitric oxide synthase （eNOS）and en-dothelin-1 （ET-1）in the lung tissue of rats were assayed by immunohistochemical method.Results：Compared to the control group,mPAP,RVSP,RVHI,WA% and WV% were significantly increased in model group and treatment group （P〈0.05）.In the treatment group,mPAP,RVSP,WA% and WV% were lower than those of the model group （P〈0.05）,but no significant change was found in RVHI. Immunoh

文章關(guān)鍵詞：

Keyword：:Ginkgo biloba extract Monocrotaline Pulmonary arterial hypertension Endothelial nitric oxide synthase Endothelin-1

課題項(xiàng)目：廣東省科技計(jì)劃項(xiàng)目（20088010600006）

作者信息：會(huì)員可見(jiàn)

  本文關(guān)鍵詞：銀杏葉提取物對(duì)野百合堿所致肺動(dòng)脈高壓保護(hù)作用的實(shí)驗(yàn)研究，由筆耕文化傳播整理發(fā)布。