雙氫青蒿素對PC-3和DU145細胞遷移和侵襲能力的影響及機制
[Abstract]:Aim: to investigate the effect of dihydroartemisinin (DHA) on migration and invasion of PC-3 and DU145 cells and its possible mechanism. Methods: in the first part, the cells of PC-3 and DU145 were treated with different concentrations of DHA (0 渭 mol L -1, 25 渭 mol L -1, 50 渭 mol L -1 and 100 渭 mol L -1) for 48 h, respectively. Cell scratch assay and invasion assay were used to detect the migration and invasion ability of the cells. The total mRNA and protein were extracted from the cells of each group, and the transcription and expression levels of Vimentin and CDH1 were detected by fluorescence quantitative PCR and Western blot assay. Part two: PC-3 and DU145 cells were used to establish implanted tumor model in nude mice. Eighteen nude mice were randomly divided into two groups: control group (1mL/kg) (DHA200 渭 mol/kg). Immunohistochemical (SP) method was used to detect the expression of Vimentin and CDH1. Results: DHA at different concentrations (25 渭 mol L-1 and 100 渭 mol L-1) significantly inhibited the migration and invasion of PC-3 and DU145 (P0.05), compared with the control group. DHA at different concentrations could significantly inhibit Vimentin and promote the transcription and expression of CDH1 (P0.05) .25 渭 mol L-1 DHA could inhibit Vimentin and promote CDH1 protein expression. Immunohistochemical results showed that the expression of CDH1 in DHA group was higher than that in control group and solvent group (P0.05), and the expression of Vimentin was lower than that in control group and solvent group (P0.05). Conclusion: different concentrations of DHA can significantly inhibit the migration and invasion of PC-3 and DU145 cells, and the inhibition of tumor growth rate in vivo further confirms that DHA can inhibit the migration and invasion of cancer cells. From the results of immunoblotting and immunohistochemistry we speculate that DHA may inhibit the migration and invasion of cancer cells by interfering with the expression of Vimentin and CDH1 proteins.
【學位授予單位】:重慶醫(yī)科大學
【學位級別】:碩士
【學位授予年份】:2014
【分類號】:R737.25
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