發(fā)布時間：2018-06-17 05:59

  本文選題：煙曲霉 + 生物膜�。� 參考：《中國感染與化療雜志》2014年04期

【摘要】：目的通過在體外復(fù)制煙曲霉的生物膜模型,研究綠原酸(chlorogenic acid,CRA)、異綠原酸(isochlorogenic acid,IRC)對生物膜的影響。方法選取臨床分離的煙曲霉構(gòu)建體外生物膜模型,微量液體稀釋法測定最低抑菌濃度(MIC),將菌株分為空白對照組、CRA實驗組(1 024 mg/L、512 mg/L、256 mg/L),IRC實驗組(1 000 mg/L、500 mg/L、250 mg/L),用掃描電子顯微鏡(scanning electron microscopy,SEM)、激光共聚焦掃描顯微鏡(laser confocal scanning microscope,CLSM)定性觀察生物膜;結(jié)晶紫染色法半定量生物膜量。結(jié)果 37℃培養(yǎng)24 h煙曲霉形成早期生物膜,48 h形成成熟期生物膜。SEM及CLSM觀察到實驗組煙曲霉生物膜均較其相應(yīng)空白對照組空洞、稀疏,但菌絲未見明顯減少,死活菌數(shù)也無明顯差別。CRA、IRC實驗組早期結(jié)晶紫半定量結(jié)果為1.05±0.19、1.14±0.26、0.99±0.14、1.39±0.06、1.41±0.06、1.60±0.04,均少于空白對照組1.91±0.17,差異具有統(tǒng)計學(xué)意義(P0.05);CRA、IRC實驗組晚期結(jié)晶紫半定量結(jié)果為2.25±0.05、2.27±0.05、2.31±0.03、2.26±0.02、2.27±0.02、2.29±0.04,少于空白對照組2.36±0.01,差異具有統(tǒng)計學(xué)意義(P0.05)。結(jié)論CRA、IRC對體外煙曲霉生物膜形成有抑制作用。
[Abstract]:Objective to study the effect of Lv Yuan acid chlorogenic acidogenic acid on biofilm by replicating the biofilm model of Aspergillus fumigatus in vitro. Methods an in vitro biofilm model was established by clinical isolation of Aspergillus fumigatus. Microliquid dilution method was used to determine the minimum inhibitory concentration (MIC), and the strains were divided into two groups: the control group: 1 024 mg / L 1 024 mg / L 512mg / L 256mg / L ~ 256mg / L ~ (-1). The biofilm was observed qualitatively by scanning electron microscope (SEM) and laser confocal scanning microscopeCLSM3 (1 000 mg / L ~ (- 500) mg 路L ~ (-1), scanning electron microscope (SEM), scanning electron microscope (SEM) and laser confocal scanning microscopeCLSM (laser confocal scanning microscope). Semi-quantitative biofilm volume by crystal violet staining. Results the biofilm of Aspergillus fumigatus was formed at 37 鈩，

本文編號：2029986