本文選題：冰凍組織芯片 + 模具　； 參考：《天津醫(yī)科大學》2006年碩士論文

【摘要】：目的: 應用自制模具進行冰凍組織芯片的研制及可行性探討,為簡單、經(jīng)濟、規(guī)范地進行蛋白及基因原位分析提供一種新方法。 方法: 收集天津醫(yī)科大學總醫(yī)院、天津市第一中心醫(yī)院及天津市胸科醫(yī)院2004年3月到2005年3月新鮮原發(fā)性肺癌手術標本19例,癌旁正常肺組織5例。應用自制模具制備48點陣冰凍組織芯片。并經(jīng)常規(guī)HE及EMA免疫組化染色,對其在形態(tài)學及基因表達分析方面的可行性進行評價。 結(jié)果: 1.冰凍組織芯片OCT模塊和HE切片的觀察:模塊上的組織芯排列基本整齊,組織芯無缺損,芯與芯之間無擠壓扭曲現(xiàn)象;HE切片鏡下每一組織芯中均可見具有診斷意義的腫瘤組織結(jié)構(gòu),所有組織中,細胞核呈藍色,細胞質(zhì)呈紅色,顏色鮮艷,核漿對比清晰,細胞的形態(tài)結(jié)構(gòu)基本清楚。 2.免疫組化染色觀察:鏡下組織芯有2點不完全脫片,未脫片的組織形態(tài)完好,背景清楚,胞漿不同程度表達EMA,呈棕黃色顆粒狀。 3.冰凍組織芯片EMA免疫組化染色結(jié)果與普通冰凍切片免疫組化結(jié)果比較,符合率為94.7%(18/19),二者比較差異無統(tǒng)計學意義(p0.05)。 結(jié)論: 1.我們冰凍組織芯片的研制方法是可行的,完全可以用于形態(tài)學觀察和蛋白表
[Abstract]:Objective: to study the feasibility of frozen tissue microarray by using self-made mould, and to provide a new method for protein and gene in situ analysis in a simple, economical and standard way. Methods: 19 fresh specimens of primary lung cancer were collected from Tianjin Medical University General Hospital, Tianjin first Central Hospital and Tianjin chest Hospital from March 2004 to March 2005. There were 5 cases of normal lung tissue adjacent to cancer. A 48-lattice frozen tissue chip was fabricated by using a self-made mold. The feasibility of morphological and gene expression analysis was evaluated by routine HE and EMA immunohistochemical staining. Results: 1. Observation of frozen tissue chip Oct module and HE section: the tissue core was arranged neatly on the module, and there was no defect in the tissue core. The tumor tissue structure with diagnostic significance was found in each tissue core under HE section. In all tissues, the nucleus was blue, the cytoplasm was red, the color was bright, and the nuclear and cytoplasm were clearly contrasted. The morphology and structure of cells are basically clear. 2. Immunohistochemical staining showed that there were 2 points of incomplete strip in the tissue core under microscope. The tissue morphology of the tissue was intact and the background was clear. The cytoplasm expressed EMA in different degree and was brown granular. The results of frozen tissue microarray EMA immunohistochemical staining were compared with those of ordinary frozen sections. The coincidence rate was 94.7% (18 / 19). There was no significant difference between the two groups (p 0.05). Conclusion: 1. It is feasible to develop the frozen tissue microarray, which can be used for morphological observation and protein table.
【學位授予單位】：天津醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R361

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