国产伦乱,一曲二曲欧美日韩,AV在线不卡免费在线不卡免费,搞91AV视频

當(dāng)前位置:主頁 > 醫(yī)學(xué)論文 > 病理論文 >

低氧及運(yùn)動對骨骼肌中低氧誘導(dǎo)因子影響的實(shí)驗(yàn)研究

發(fā)布時(shí)間:2018-06-16 01:14

  本文選題:高原 + 低氧 ; 參考:《第一軍醫(yī)大學(xué)》2005年博士論文


【摘要】:目的:研究低氧及低氧復(fù)合運(yùn)動情況下大鼠體重、骨骼肌顯微、超微結(jié)構(gòu)及低氧誘導(dǎo)因子發(fā)生變化的規(guī)律,探討在低氧條件下提高機(jī)體低氧習(xí)服能力的途徑和可能的機(jī)制。 方法:分別建立大鼠低氧及正常氧情況下運(yùn)動模型:雄性SD大鼠80只,隨機(jī)分為4組,即常氧安靜組(A)、常氧運(yùn)動組(B)、低氧安靜組(C)、低氧運(yùn)動組(D)。大鼠運(yùn)動采用跑臺模型,速度設(shè)定為25m/min,檢測在第3天(急性低氧)、第7天、第10天與第14天(慢性低氧)各組大鼠體重(W)變化,光鏡和電鏡分別觀察骨骼肌顯微和超微結(jié)構(gòu)的改變,對骨骼肌中低氧誘導(dǎo)因子-1(HIF-1)的表達(dá)運(yùn)用實(shí)時(shí)熒光PCR(fluorescence quantitative PCR FQ-PCR)、表面加強(qiáng)激光解析電離化芯片技術(shù)(Surface-enhanced LaserDesorption/Ionization SELDI)進(jìn)行定量分析;采用2×4×2析因分析,分組變量分別為氧含量、運(yùn)動與否以及時(shí)間,其中氧含量為2個水平:正常氧和低氧;運(yùn)動情況有2個水平:安靜與運(yùn)動;時(shí)間有4個水平,分別為3天、7天、10天、14天。分別研究低氧及運(yùn)動的不同階段對骨骼肌形態(tài)結(jié)構(gòu)的影響以及骨骼肌中低氧誘導(dǎo)因子表達(dá)的變化規(guī)律,分析其相互作用,探討骨骼肌結(jié)構(gòu)、功能改變的發(fā)生機(jī)制及演變過程,尋找在低氧條件下促進(jìn)低氧習(xí)服能力的途徑,探討其發(fā)生的可能的機(jī)制。 結(jié)果:單純低氧組大鼠體重在試驗(yàn)前后有所降低,而正常氧安靜組、常氧運(yùn)動組及低氧運(yùn)動組大鼠體重均有不同程度的增加,與低氧安靜組比較有顯著意義(p0.05)。在肌肉形態(tài)學(xué)方面,單純低氧組大鼠急性期光鏡下肌纖維形態(tài)尚正常,電鏡下已出現(xiàn)線粒體體積增大、腫脹,嵴稍腫脹、嵴內(nèi)腔擴(kuò)大,嵴紊亂;在慢性期光鏡下出現(xiàn)肌纖維萎縮,電鏡下可見線粒體輕度腫脹,水性變及空泡化,
[Abstract]:Aim: to study the changes of body weight, skeletal muscle microstructure, ultrastructure and hypoxia inducible factors in hypoxic and hypoxic combined exercise rats, and to explore the ways and possible mechanisms of enhancing hypoxic acclimatization under hypoxic conditions. Methods: 80 male Sprague-Dawley (SD) rats were randomly divided into four groups: normal oxygen quiet group, normoxic exercise group, hypoxia quiet group and hypoxic exercise group. The rats used a treadmill model with a speed of 25 m / min. The changes of body weight were measured on day 3 (acute hypoxia, day 7, day 10 and day 14 (chronic hypoxia). The changes of microstructure and ultrastructure of skeletal muscle were observed by light microscope and electron microscope respectively. The expression of hypoxia inducible factor-1 (HIF-1) in skeletal muscle was quantitatively analyzed by real-time fluorescence quantitative FQ-PCRN, surface-enhanced laser desorption / ionization chip technique (Surface-enhanced LaserDesorptionIonization SELDI). Using 2 脳 4 脳 2 factorial analysis, the grouped variables are oxygen content, exercise or not, and time, in which oxygen content is 2 levels: normal oxygen level and hypoxia level; exercise condition has 2 levels: quiet and exercise; time has 4 levels. They were 3 days, 7 days, 10 days and 14 days. The effects of hypoxia and exercise on skeletal muscle morphology and the expression of hypoxia inducible factor in skeletal muscle were studied, and the mechanism and evolution of skeletal muscle structure and function were discussed. To find ways to promote hypoxic acclimatization under hypoxia conditions and explore the possible mechanism of its occurrence. Results: the body weight of rats in hypoxic group was decreased before and after the experiment, but the body weight of rats in normal oxygen quiet group, normoxic exercise group and hypoxic exercise group increased in varying degrees, which was significantly higher than that in hypoxic quiet group (P 0.05). In terms of muscle morphology, the morphology of muscle fibers was normal under light microscope in the acute phase of hypoxic rats. Mitochondria volume increased, swelling, cristae slightly swollen, cristal cavity enlarged and cristal disorder appeared under electron microscope. Myofibril atrophy was observed under the light microscope in chronic phase, slight swelling of mitochondria, waterborne degeneration and vacuolation were observed under electron microscope.
【學(xué)位授予單位】:第一軍醫(yī)大學(xué)
【學(xué)位級別】:博士
【學(xué)位授予年份】:2005
【分類號】:R363;R87

【引證文獻(xiàn)】

中國期刊全文數(shù)據(jù)庫 前1條

1 謝康玲;劉遂心;蔡穎;張文亮;;長期中等強(qiáng)度運(yùn)動對小鼠骨骼肌HIF-1α mRNA的表達(dá)及葡萄糖轉(zhuǎn)運(yùn)的影響[J];中國康復(fù)醫(yī)學(xué)雜志;2012年06期

,

本文編號:2024528

資料下載
論文發(fā)表

本文鏈接:http://lk138.cn/yixuelunwen/binglixuelunwen/2024528.html


Copyright(c)文論論文網(wǎng)All Rights Reserved | 網(wǎng)站地圖 |

版權(quán)申明:資料由用戶c0415***提供,本站僅收錄摘要或目錄,作者需要刪除請E-mail郵箱bigeng88@qq.com
久久久精品无忧| 欧美日韩的一区二区区| 久久蜜日韩精| 日本一区二区性学堂| www.91av秘桃| 亚洲tv在线一区| 日韩精品二区页| 中文字幕精品一区二区女同| 亚洲av中文无码一区二区三区| 一区二区三 AV| 国产精品18久久久久久白浆动漫| 婷婷五月天在线播放av| 久久精品国产亚洲麻豆| www.午夜福利| 91精品播放中文音声颅内| 久热6在线观看| 伊伊高清美女AV在线| av女优日韩资源| 中文无码综合视频在线播放| 天天插天天摸天天日| 日韩www91视频| 久久亚州精品少妇| 欧美精品一区二区舔舔| 亚洲午夜成人性爱网站| 久久午夜福利片交换| 国产一区二区三区精华| 欧美日韩黄片免费观看一二三区| 色婷婷欧美色| 内射日本精品| 天天操天天抽天天操天天干| 插逼综合网站| 欧美人妻制服丝袜| 乱轮性爱视频| 91人人妻人人澡人人爽男同| 激情综合三| 日本亚洲亚洲国产欧美一区| 美女让大鸡巴操| www.欧美久久| 色色88久久| 黑人日韩久久久| 激情小说 乱 视频|