本文選題：高原 + 低氧　； 參考：《第一軍醫(yī)大學》2005年博士論文

【摘要】：目的:研究低氧及低氧復合運動情況下大鼠體重、骨骼肌顯微、超微結(jié)構(gòu)及低氧誘導因子發(fā)生變化的規(guī)律,探討在低氧條件下提高機體低氧習服能力的途徑和可能的機制。 方法:分別建立大鼠低氧及正常氧情況下運動模型:雄性SD大鼠80只,隨機分為4組,即常氧安靜組(A)、常氧運動組(B)、低氧安靜組(C)、低氧運動組(D)。大鼠運動采用跑臺模型,速度設定為25m/min,檢測在第3天(急性低氧)、第7天、第10天與第14天(慢性低氧)各組大鼠體重(W)變化,光鏡和電鏡分別觀察骨骼肌顯微和超微結(jié)構(gòu)的改變,對骨骼肌中低氧誘導因子-1(HIF-1)的表達運用實時熒光PCR(fluorescence quantitative PCR FQ-PCR)、表面加強激光解析電離化芯片技術(shù)(Surface-enhanced LaserDesorption/Ionization SELDI)進行定量分析;采用2×4×2析因分析,分組變量分別為氧含量、運動與否以及時間,其中氧含量為2個水平:正常氧和低氧;運動情況有2個水平:安靜與運動;時間有4個水平,分別為3天、7天、10天、14天。分別研究低氧及運動的不同階段對骨骼肌形態(tài)結(jié)構(gòu)的影響以及骨骼肌中低氧誘導因子表達的變化規(guī)律,分析其相互作用,探討骨骼肌結(jié)構(gòu)、功能改變的發(fā)生機制及演變過程,尋找在低氧條件下促進低氧習服能力的途徑,探討其發(fā)生的可能的機制。 結(jié)果:單純低氧組大鼠體重在試驗前后有所降低,而正常氧安靜組、常氧運動組及低氧運動組大鼠體重均有不同程度的增加,與低氧安靜組比較有顯著意義(p0.05)。在肌肉形態(tài)學方面,單純低氧組大鼠急性期光鏡下肌纖維形態(tài)尚正常,電鏡下已出現(xiàn)線粒體體積增大、腫脹,嵴稍腫脹、嵴內(nèi)腔擴大,嵴紊亂;在慢性期光鏡下出現(xiàn)肌纖維萎縮,電鏡下可見線粒體輕度腫脹,水性變及空泡化,
[Abstract]:Aim: to study the changes of body weight, skeletal muscle microstructure, ultrastructure and hypoxia inducible factors in hypoxic and hypoxic combined exercise rats, and to explore the ways and possible mechanisms of enhancing hypoxic acclimatization under hypoxic conditions. Methods: 80 male Sprague-Dawley (SD) rats were randomly divided into four groups: normal oxygen quiet group, normoxic exercise group, hypoxia quiet group and hypoxic exercise group. The rats used a treadmill model with a speed of 25 m / min. The changes of body weight were measured on day 3 (acute hypoxia, day 7, day 10 and day 14 (chronic hypoxia). The changes of microstructure and ultrastructure of skeletal muscle were observed by light microscope and electron microscope respectively. The expression of hypoxia inducible factor-1 (HIF-1) in skeletal muscle was quantitatively analyzed by real-time fluorescence quantitative FQ-PCRN, surface-enhanced laser desorption / ionization chip technique (Surface-enhanced LaserDesorptionIonization SELDI). Using 2 脳 4 脳 2 factorial analysis, the grouped variables are oxygen content, exercise or not, and time, in which oxygen content is 2 levels: normal oxygen level and hypoxia level; exercise condition has 2 levels: quiet and exercise; time has 4 levels. They were 3 days, 7 days, 10 days and 14 days. The effects of hypoxia and exercise on skeletal muscle morphology and the expression of hypoxia inducible factor in skeletal muscle were studied, and the mechanism and evolution of skeletal muscle structure and function were discussed. To find ways to promote hypoxic acclimatization under hypoxia conditions and explore the possible mechanism of its occurrence. Results: the body weight of rats in hypoxic group was decreased before and after the experiment, but the body weight of rats in normal oxygen quiet group, normoxic exercise group and hypoxic exercise group increased in varying degrees, which was significantly higher than that in hypoxic quiet group (P 0.05). In terms of muscle morphology, the morphology of muscle fibers was normal under light microscope in the acute phase of hypoxic rats. Mitochondria volume increased, swelling, cristae slightly swollen, cristal cavity enlarged and cristal disorder appeared under electron microscope. Myofibril atrophy was observed under the light microscope in chronic phase, slight swelling of mitochondria, waterborne degeneration and vacuolation were observed under electron microscope.
【學位授予單位】：第一軍醫(yī)大學
【學位級別】：博士
【學位授予年份】：2005
【分類號】：R363;R87

【引證文獻】

中國期刊全文數(shù)據(jù)庫 前1條

1 謝康玲;劉遂心;蔡穎;張文亮;;長期中等強度運動對小鼠骨骼肌HIF-1α mRNA的表達及葡萄糖轉(zhuǎn)運的影響[J];中國康復醫(yī)學雜志;2012年06期

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本文編號：2024528