毛畢吸蟲生物學及其自然疫源地的研究
發(fā)布時間:2018-02-22 13:01
本文關(guān)鍵詞: 毛畢屬吸蟲 尾蚴性皮炎 醫(yī)學貝類 耳蘿卜螺 自然疫源地 出處:《安徽理工大學》2007年碩士論文 論文類型:學位論文
【摘要】: 本論文主要研究了引起安徽淮南高塘湖地區(qū)尾蚴性皮炎的病原——鴨毛畢吸蟲的生物學,以及人群尾蚴性皮炎的流行情況和對本地區(qū)毛畢吸蟲自然疫源地的研究。 本文對毛畢吸蟲發(fā)育各期蟲體的收集方法進行對比研究,認為最有效的成蟲、尾蚴、毛蚴的分離、收集方法分別為:肝門靜脈及腸系膜靜脈同時灌注法、滴瓶遮光法和三角燒瓶頂管法。 本文對毛畢吸蟲生活史各期分別進行了活體觀察、顯微鏡下觀察或掃描電鏡觀察,詳細描述了其形態(tài)特征,并附有圖片。對高塘湖地區(qū)的毛畢吸蟲進行了蟲種探討。 調(diào)查了安徽淮南地區(qū)的常見醫(yī)學貝類,并通過現(xiàn)場觀察和實驗室飼養(yǎng)的方法對毛畢吸蟲中間宿主耳蘿卜螺的生態(tài)進行了細致的研究。本地的醫(yī)學貝類經(jīng)初步鑒定有8種,隸屬于腹足綱4科7屬;耳蘿卜螺在本地區(qū)分布廣泛,3月上旬開始活動,3月下旬、4月上旬為產(chǎn)卵高峰期,11月上旬成螺開始越冬。一年可繁殖3代,4~5月份孳生密度最高為468只/m~2。 本文特別對毛畢吸蟲尾蚴的生物學進行了研究,發(fā)現(xiàn)尾蚴檢出率隨氣溫而變化,呈現(xiàn)明顯的季節(jié)性消長,4~10月均可檢出尾蚴,以7月檢出率最高,為1.12%;尾蚴具有強烈的趨光性,壽命可達52 h,毛畢吸蟲尾蚴時齡在40 h之前對宿主有感染力,其后感染力完全喪失;毛畢吸蟲尾蚴的逸出與溫度和光照等環(huán)境因子有關(guān),在20~25℃有光照的情況下均有尾蚴逸出。 對高塘湖地區(qū)的調(diào)查顯示,此地區(qū)存在毛畢吸蟲的終宿主,其感染率分別是家鴨35.20%,野鴨56.25%;存在毛畢吸蟲的中間宿主耳蘿卜螺,其感染率為1.03%;常在此地區(qū)勞作的農(nóng)民、漁民毛畢吸蟲尾蚴性皮炎出現(xiàn)率為79.37%,皮炎的發(fā)生與性別無關(guān)。高塘湖地區(qū)存在毛畢吸蟲的自然疫源地。
[Abstract]:This paper mainly studied the biology of cercariosis caused by cercariasis in Gaotang Lake area, Huainan, Anhui Province, and the prevalence of cercaria dermatitis in population and the natural foci of flutter fluke in this area. In this paper, a comparative study was made on the collection methods of adult, cercariae and cercariae in different stages of development of paragonimiasis. The collection methods were as follows: simultaneous perfusion of hepatic portal vein and mesenteric vein, respectively, for the separation of adult worms, cercariae and cercariae. Drop bottle shading method and triangle flask jacking method. In this paper, the living body observation, microscope observation or scanning electron microscope observation were carried out on each stage of the life cycle of Trichobidium trichome. The morphological characteristics of the trematode were described in detail, and the pictures were attached. The species of Trichobidium in Gaotang Lake area were discussed in this paper. The common medical shellfish in Huainan area of Anhui Province were investigated, and the ecology of turnip snails, an intermediate host of Trichobilharca, was studied in detail by field observation and laboratory feeding. Eight species of local medical shellfish were preliminarily identified. It belongs to 4 families and 7 genera of Gastropoda, and is widely distributed in this area. It began to be active on early March. On late March and early April, it was the peak period of spawning, and the adult snail began to overwintering in early April. The highest breeding density was 468 / m ~ (2) / m ~ (2) in May and 3 generations per year. In this paper, the biology of cercariae was studied. It was found that the detectable rate of cercariae varied with air temperature, and cercariae could be detected from 4 to October. The detection rate of cercariae in July was the highest (1.12%), and cercariae had strong phototaxis. The life span of cercariae reached 52 h, and the cercariae of Trichobilharzia cercariae were infected with the host before 40 h and then lost their infectivity completely. The escape of cercariae was related to environmental factors such as temperature and light, and cercariae escaped at 2025 鈩,
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