發(fā)布時(shí)間：2018-02-16 05:36

  本文關(guān)鍵詞： 內(nèi)皮型脾臟基質(zhì)細(xì)胞 造血干細(xì)胞 分化 調(diào)節(jié)性樹突狀細(xì)胞 白細(xì)胞介素10 T細(xì)胞　出處：《浙江大學(xué)》2005年博士論文　論文類型：學(xué)位論文

【摘要】：樹突狀細(xì)胞(dendritic cells,DCs)是體內(nèi)功能最強(qiáng)的抗原遞呈細(xì)胞。在過去的十年中,多數(shù)研究者的興趣是集中在DC是如何激發(fā)免疫應(yīng)答的研究上,然而,近幾年的工作顯示DCs不但具有激發(fā)免疫反應(yīng)的能力,而且,還可以下調(diào)免疫反應(yīng)或者誘導(dǎo)免疫耐受的產(chǎn)生�，F(xiàn)在普遍認(rèn)為,DC在激活na(?)ve T細(xì)胞和誘導(dǎo)耐受中都扮演關(guān)鍵的角色。DCs既可以誘導(dǎo)中樞耐受又可以誘導(dǎo)外周耐受,特別是DCs在誘導(dǎo)外周耐受中的作用越來越受到重視。最近,具有免疫負(fù)向調(diào)節(jié)功能的調(diào)節(jié)性DCs(regulatory dendritic cells)被陸續(xù)發(fā)現(xiàn),并成為這一研究領(lǐng)域的新熱點(diǎn)。 目前對調(diào)節(jié)性DCs的研究主要集中在對其起源和發(fā)育的研究上,所采用的方法多數(shù)是用免疫抑制性細(xì)胞因子誘導(dǎo)產(chǎn)生的,如在常規(guī)誘導(dǎo)DCs的培養(yǎng)基中加入IL-10或TGF-β。但這類方法過于簡化,不能反映在體的實(shí)際分化過程,因此產(chǎn)生的DCs的特性是否能代表一種天然的細(xì)胞群體并不十分明確。 造血細(xì)胞的體內(nèi)發(fā)育過程是在一定的微環(huán)境的作用下進(jìn)行的。所謂的微環(huán)境(microenvironment)或“龕位”(niche)是由成纖維細(xì)胞、巨噬細(xì)胞、內(nèi)皮細(xì)胞、脂肪細(xì)胞等基質(zhì)細(xì)胞和它們分泌的細(xì)胞外基質(zhì)組成的,為造血細(xì)胞的發(fā)育提供各種必需的外源性信號的發(fā)育場所。不同的器官有不同的微環(huán)境,同一器官的不同區(qū)域的亞微環(huán)境也不同。不同的微環(huán)境可誘導(dǎo)產(chǎn)生不同的種類或功能特征的細(xì)胞。如骨髓的造血微環(huán)境。因此,脫離微環(huán)境來研究造血細(xì)胞的發(fā)育所得結(jié)論不可能反映機(jī)體的真實(shí)情況。 脾臟是機(jī)體最大的外周免疫器官,又是哺乳動(dòng)物的造血器官,并且小鼠脾臟終身保持造血功能。經(jīng)典的脾結(jié)節(jié)形成實(shí)驗(yàn)證明,脾臟的微環(huán)境不但具有支持造血的作用,而
[Abstract]:Dendritic cells ( DCs ) are the most potent antigen - presenting cells in vivo . In the past decade , the interest of most researchers has been focused on how DCs can stimulate immune responses . However , recent studies have shown that DCs play a key role in activating na ( ? ) ve T cells and inducing tolerance . It is generally believed that DCs play a key role in activating na ( ? ) ve T cells and inducing tolerance . DCs can both induce central tolerance and induce peripheral tolerance , especially DCs in inducing peripheral tolerance . Recently , regulatory DCs with immune negative regulatory functions have been recognized . cells ) have been found in succession and become a new focus in this field of research . At present , the study of regulatory DCs is mainly focused on the study of the origin and development of DCs , most of which are induced by immunosuppressive cytokines , such as the addition of IL - 10 or TGF - 尾 in the culture medium of conventional induced DCs , but this method is too simplified to reflect the actual differentiation of DCs , and thus whether the nature of DCs produced represents a natural population of cells is not very clear . In vivo development of hematopoietic cells is carried out under the action of a certain microenvironment . The so - called microenvironment or niche consists of stromal cells , such as fibroblasts , macrophages , endothelial cells , fat cells , and extracellular matrix , and provides various necessary exogenous signals for the development of hematopoietic cells . Different organs have different microenvironment , and the sub - microenvironment of different regions of the same organ is different . The spleen is the largest peripheral immune organ of the organism , and is the hematopoietic organ of the mammal , and the spleen of the mouse keeps the hematopoietic function for a lifetime . The classical spleen nodule formation experiment proves that the microenvironment of the spleen not only has the function of supporting the hematopoietic function , but also

【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2005
【分類號】：R392

【共引文獻(xiàn)】

相關(guān)博士學(xué)位論文 前2條

1 張明徽;脾基質(zhì)細(xì)胞對造血前體細(xì)胞的定向誘導(dǎo)作用及對樹突狀細(xì)胞生物學(xué)特性的影響[D];第二軍醫(yī)大學(xué);2002年

2 郭振紅;凋亡信號和免疫微環(huán)境對樹突狀細(xì)胞的免疫調(diào)控作用[D];第二軍醫(yī)大學(xué);2005年

相關(guān)碩士學(xué)位論文 前1條

1 李志宏;脾臟抗原提呈細(xì)胞在多器官功能障礙綜合征免疫失衡中作用的研究[D];中國人民解放軍軍醫(yī)進(jìn)修學(xué)院;2003年

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本文編號：1514778