本文關(guān)鍵詞：兩種脫礦體系制備人工齲損的病理學(xué)比較，由筆耕文化傳播整理發(fā)布。

兩種脫礦體系制備人工齲損的病理學(xué)比較

Pathological Comparison of Two Type of Demineralization Solutions on the Production of Initial Artificial Enamel Carious Lesion

[1] [2] [3] [4]

CHU Jin-pu , HAO Yu-qing , LI Ji-yao , ZHOU Xue-dong （ 1. Department of Cariology and Endodontology, Zhengzhou University, Zhengzhou 450052, China ; 2. Key Laboratory of

[1]鄭州大學(xué)口腔醫(yī)學(xué)院牙體牙髓病科,鄭州450052; [2]四川大學(xué)口腔生物醫(yī)學(xué)工程教育部重點(diǎn)實(shí)驗(yàn)室,成都610041

文章摘要：目的評價(jià)兩種不同脫礦體系在人牙釉質(zhì)與牛牙釉質(zhì)形成人工齲樣損害的情況，確定模擬早期齲實(shí)驗(yàn)研究的脫礦系統(tǒng)。方法兩種脫礦體系：部分飽和酸緩沖溶液和酸性凝膠溶液，分別在人牙釉質(zhì)與牛牙釉質(zhì)制備人工早期釉質(zhì)齲損，，采用偏振光顯微鏡和掃描電子顯微鏡觀察實(shí)驗(yàn)人工齲的組織病理學(xué)變化。結(jié)果部分飽和的酸緩沖體系制備的人工齲損都具有完整的表層和表層下脫礦，牛牙釉質(zhì)除了脫礦深度大于人牙釉質(zhì)外，其它病理學(xué)特點(diǎn)都類似于人牙釉質(zhì)，與早期自然齲損相似。而凝膠液脫礦體系形成的人工齲損不管是人牙還是牛牙都沒有明顯的表層結(jié)構(gòu)，實(shí)驗(yàn)牙表面多出現(xiàn)被腐蝕或溶解。結(jié)論部分飽和的酸緩沖液脫礦系統(tǒng)形成的人工齲損病理學(xué)變化更接近于自然齲；與人牙具有相似化學(xué)組成的牛牙很適合代替人牙來檢測脫礦和再礦化的效果。

Abstr：Objective To determine the effect of two type of demineralization solutions on the production of artificial carious lesion in bovine enamel and human enamel in vitro. Methods A partially saturated acidic buffer solution and an acidifed hydroxyethylcellulose gel system were used as decalcifying media for the preparation of initial artificial carious lesion in bovine enamel and human enamel. After demineralization, these enamel specimens was observed by microscope and scaning electron microscope and compared in histological pathology. Results The results indicated all specimens in a partially saturated acidic buffer solution could morphologically form subsurface demineralization, which were the same as the early smooth enamel caries. Lesions in bovine teeth were pathologically similar to those in human teeth, except that the lesions depth was deeper than those in human teeth. However, In gel, regular subsurface lesions were hardly produced in all specimens. They were either eroded or dissolved. Conclusion It was concluded that caries-like lesions could be consistently produced in bovine enamel with a partially saturated acidic buffer solution. So this demineralization system can be use for reproducible preparation of artificial carious lesions in dental enamel.

文章關(guān)鍵詞：

Keyword：:Demineralization solutions Artificial caries Pathological study

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課題項(xiàng)目：國家自然科學(xué)基金重點(diǎn)資助項(xiàng)目（30430800）.

  本文關(guān)鍵詞：兩種脫礦體系制備人工齲損的病理學(xué)比較，由筆耕文化傳播整理發(fā)布。