基于二氧化鋯納米顆粒載藥體系的制備及應(yīng)用研究
發(fā)布時(shí)間：2019-01-17 13:16
【摘要】：文章設(shè)計(jì)了第一個(gè)基于空心氧化鋯納米球的智能納米容器(HMZSs-1),主要由中空介孔氧化鋯納米球(HMZSs)作為納米支架和雙穩(wěn)態(tài)準(zhǔn)輪烷作為超分子開關(guān)組成。使用HMZSs取代介孔二氧化硅納米支架的顯著優(yōu)點(diǎn)是HMZSs可以經(jīng)受熱堿性反應(yīng)環(huán)境,其提供了用于官能化的新穎策略,并且因此通過簡單和可行的裝配程序?qū)崿F(xiàn)了雙pH響應(yīng)的控制釋放功能。本文首先合成了雙(2-氨基乙基)縮酮(PBAEK),并通過單保護(hù)將其改性至HMZSs的表面。通過透射電子顯微鏡(TEM)、氮?dú)馕矫摳降葴鼐�(BET)、傅里葉紅外光譜儀(FTIR)、固體核磁共振儀(SS 13C NMR)和熱重分析儀(TG)等儀器對HMZSs的表面改性及結(jié)構(gòu)進(jìn)行了表征。使用了紫外分光光度計(jì)(UV)對HMZSs-1的釋放性能進(jìn)行了研究。發(fā)現(xiàn)在中性溶液下,葫蘆[7]脲(CB[7])大環(huán)化合物與PBAEK絡(luò)合形成[2]準(zhǔn)輪烷作為超分子開關(guān),阻斷孔口并防止藥物的泄漏。當(dāng)溶液調(diào)節(jié)至堿性時(shí),CB[7]大環(huán)化合物作為蓋,與PBAEK鏈分離,并且由于離子-偶極相互作用的顯著降低而打開開關(guān),釋放效率達(dá)到81%。當(dāng)在酸性條件下,由于縮酮基團(tuán)的斷裂,PBAEK鏈被破壞,導(dǎo)致超分子開關(guān)的打開和隨后的藥物的釋放,釋放效率達(dá)到82%。具有酸堿雙響應(yīng)的控釋特性的HMZSs-1預(yù)期應(yīng)用于許多領(lǐng)域。本文中,研究了羅丹明B(RhB)負(fù)載的HMZSs-1作為藥物遞送系統(tǒng)的可行性。體外細(xì)胞研究表明,裝載RhB的HMZSs-1可以容易地被SMMC-7721細(xì)胞攝取,在細(xì)胞內(nèi)快速釋放RhB,證明它們具有應(yīng)用于藥物化療的潛力。
[Abstract]:The first intelligent nano-vessel (HMZSs-1) based on hollow zirconia nanospheres is designed in this paper, which consists of hollow mesoporous zirconia nanospheres (HMZSs) as nano-scaffolds and bistable quasirotane as supramolecular switches. The remarkable advantage of using HMZSs to replace mesoporous silica nanospheres is that HMZSs can withstand hot-alkaline reaction environments which provide novel strategies for functionalization. Therefore, the control and release function of double pH response is realized by a simple and feasible assembly program. In this paper, bis (2-aminoethyl) ketone (PBAEK), was synthesized and modified to the surface of HMZSs by single protection. Adsorption and desorption of nitrogen by transmission electron microscope (TEM),) isotherm (BET), Fourier transform infrared spectrometer (FTIR), The surface modification and structure of HMZSs were characterized by SS 13 C NMR and thermogravimetric analyzer (TG). The release performance of HMZSs-1 was studied by ultraviolet spectrophotometer (UV). It is found that in neutral solution, the macrocyclic compound of gourd [7] urea (CB [7]) complexed with PBAEK to form [2] quasiroalkane as a supramolecular switch to block the pore opening and prevent the leakage of the drug. When the solution is adjusted to alkalinity CB [7] macrocyclic compounds act as capping and separate from PBAEK chain and switch on due to the significant decrease of ion-dipole interaction. The release efficiency reaches 81%. Under acidic conditions, the PBAEK chain is destroyed due to the breaking of the Ketal group, which leads to the opening of supramolecular switch and the subsequent release of the drug, and the release efficiency is up to 82%. HMZSs-1 with acid-base dual-response controlled release properties is expected to be applied in many fields. In this paper, the feasibility of Rhodamine B (RhB) loaded HMZSs-1 as drug delivery system is studied. In vitro cellular studies showed that HMZSs-1 loaded with RhB could be easily ingested by SMMC-7721 cells. The rapid release of RhB, in cells proved their potential for drug chemotherapy.
【學(xué)位授予單位】：南京理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TQ460.6
本文編號：2410096
[Abstract]:The first intelligent nano-vessel (HMZSs-1) based on hollow zirconia nanospheres is designed in this paper, which consists of hollow mesoporous zirconia nanospheres (HMZSs) as nano-scaffolds and bistable quasirotane as supramolecular switches. The remarkable advantage of using HMZSs to replace mesoporous silica nanospheres is that HMZSs can withstand hot-alkaline reaction environments which provide novel strategies for functionalization. Therefore, the control and release function of double pH response is realized by a simple and feasible assembly program. In this paper, bis (2-aminoethyl) ketone (PBAEK), was synthesized and modified to the surface of HMZSs by single protection. Adsorption and desorption of nitrogen by transmission electron microscope (TEM),) isotherm (BET), Fourier transform infrared spectrometer (FTIR), The surface modification and structure of HMZSs were characterized by SS 13 C NMR and thermogravimetric analyzer (TG). The release performance of HMZSs-1 was studied by ultraviolet spectrophotometer (UV). It is found that in neutral solution, the macrocyclic compound of gourd [7] urea (CB [7]) complexed with PBAEK to form [2] quasiroalkane as a supramolecular switch to block the pore opening and prevent the leakage of the drug. When the solution is adjusted to alkalinity CB [7] macrocyclic compounds act as capping and separate from PBAEK chain and switch on due to the significant decrease of ion-dipole interaction. The release efficiency reaches 81%. Under acidic conditions, the PBAEK chain is destroyed due to the breaking of the Ketal group, which leads to the opening of supramolecular switch and the subsequent release of the drug, and the release efficiency is up to 82%. HMZSs-1 with acid-base dual-response controlled release properties is expected to be applied in many fields. In this paper, the feasibility of Rhodamine B (RhB) loaded HMZSs-1 as drug delivery system is studied. In vitro cellular studies showed that HMZSs-1 loaded with RhB could be easily ingested by SMMC-7721 cells. The rapid release of RhB, in cells proved their potential for drug chemotherapy.
【學(xué)位授予單位】：南京理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TQ460.6
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相關(guān)碩士學(xué)位論文 前1條
1 宮光彩;基于二氧化鋯納米顆粒載藥體系的制備及應(yīng)用研究[D];南京理工大學(xué);2017年
，本文編號：2410096
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