發(fā)布時間：2018-03-04 02:21

  本文選題：海帶　切入點：基因工程　出處：《海洋湖沼通報》2017年02期 　論文類型：期刊論文

【摘要】：本文以海帶(Saccharina japonica)配子體、配子體克隆及幼孢子體為實驗材料,研究了不同發(fā)育時期的海帶對草甘膦、草丁膦、氯霉素的敏感性,經(jīng)SPSS軟件計算出一定時間內(nèi)3種選擇試劑對不同發(fā)育時期海帶的半致死劑量(LD50)。結(jié)果表明:海帶配子體及配子體克隆對草甘膦和草丁膦極為敏感,對氯霉素較為敏感。4d時,草甘膦和草丁膦對海帶配子體的LD50分別為8.19μg/mL和19.27μg/mL,對海帶配子體克隆的LD50分別為73.69μg/mL和39.08μg/mL;而氯霉素對海帶配子體及配子體克隆4天的LD50則高達742.00μg/mL和701.51μg/mL。海帶幼孢子體僅對草甘膦極為敏感,4d的LD50為40.93μg/mL;對草丁膦和氯霉素較為敏感,4d的LD50分別為214.51μg/mL和907.86μg/mL。該結(jié)果說明草甘膦和草丁膦適合作為以海帶配子體和配子體克隆為實驗材料的基因工程選擇試劑,草甘膦則更適合以海帶幼孢子體為實驗材料的基因工程選擇試劑。
[Abstract]:In this paper, the sensitivity of Laminaria japonica to glyphosate, butylphosphine and chloramphenicol at different developmental stages was studied by using gametophyte, gametophyte cloning and juvenile sporophyte as experimental materials. The results showed that the gametophyte and gametophyte clones of Laminaria japonica were very sensitive to glyphosate and glyphosate and were sensitive to chloramphenicol for 4 d. The LD50 of glyphosate and glyphosate to gametophyte of Laminaria japonica were 8.19 渭 g / mL and 19.27 渭 g / mL, respectively. The LD50 of gametophyte clone of Laminaria japonica were 73.69 渭 g / mL and 39.08 渭 g / mL, respectively. The LD50 of gametophyte and gametophyte cloned by chloramphenicol for 4 days was 742.00 渭 g / mL and 701.51 渭 g / mL, respectively. The LD50 for 4 days was 40.93 渭 g / mL for glyphosate and 214.51 渭 g / mL for glyphosate and 907.86 渭 g / mL for chloramphenicol for 4 days, respectively. The results showed that glyphosate and glyphosate were suitable for gene engineering selection with gametophyte and gametophyte clones of Laminaria japonica. Glyphosate is more suitable for genetic engineering of laminaria japonica.
【作者單位】： 中國海洋大學海洋生命學院;
【基金】：褐藻甘露糖和甘露醇合成通路基因的克隆與功能分析(41376143) 山東省大型經(jīng)濟海藻種質(zhì)資源庫構(gòu)建及其應(yīng)用(2014GGE29091) 廣東省特色海洋中藥藻類資源發(fā)掘與開發(fā)利用(2013B021100008)資助
【分類號】：S917.3

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本文編號：1563727