發(fā)布時間：2018-03-03 03:12

  本文選題：條銹病　切入點：抗病基因推導(dǎo)　出處：《植物病理學(xué)報》2017年02期 　論文類型：期刊論文

【摘要】：條銹病是我國小麥種植區(qū)域重要的流行病害之一。病原菌群體中流行小種改變常導(dǎo)致抗病品種"喪失"抗銹性。構(gòu)建單基因近等基因系對于抗條銹病基因鑒定分析、病原菌小種毒性變異監(jiān)測具有重要的意義。本研究以高感病冬麥品種銘賢169為遺傳背景、尤皮Ⅱ號為抗條銹病基因供體轉(zhuǎn)育構(gòu)建單基因近等基因系。采用基因推導(dǎo)并結(jié)合遺傳分析方法,明確銘賢169*6/尤皮Ⅱ號的近等基因株系N27和N36所含抗條銹病基因的個數(shù),并利用與YrJu4緊密連鎖的SSR分子標(biāo)記對鑒定株系進(jìn)行分子檢測�；�因推導(dǎo)分析結(jié)果表明,N27和N36近等基因株系可能含有YrJu3或YrJu4,或YrJu3+YrJu4。用菌系CY17和CY26對利用N27和N36株系構(gòu)建的群體進(jìn)行遺傳分析,發(fā)現(xiàn)這兩個株系對CY17和CY26的抗性都是受1對顯性基因控制。分子標(biāo)記檢測結(jié)果表明,N27和N36株系都可擴增出與來源于抗性基因供體尤皮Ⅱ號的YrJu4相同的型帶。因此,來源于小麥條銹菌鑒別品種尤皮Ⅱ號的抗病基因YrJu4已成功轉(zhuǎn)育到輪回親本冬麥品種銘賢169中,N27和N36株系是以銘賢169為遺傳背景的YrJu4的單基因近等基因系,即銘賢169*6/YrJu4。
[Abstract]:Stripe rust is one of the most important epidemic diseases in wheat planting area in China. The change of epidemic species in pathogen population often leads to "loss" of resistance to rust in disease-resistant varieties. The identification and analysis of single gene near-isogenic lines for resistance to stripe rust are carried out. It is important to monitor the virulence variation of pathogen races. The genetic background of this study was Ming-Xian 169, a highly susceptible winter wheat variety. Yupi 鈪，

本文編號：1559285