發(fā)布時(shí)間：2018-08-28 19:16

【摘要】：目的:通過流式細(xì)胞術(shù)分別對經(jīng)腹腔鏡和開腹下行胃癌D2根治術(shù)患者的腹腔沖洗液中的腫瘤脫落細(xì)胞進(jìn)行檢測,對比分析兩組腫瘤脫落細(xì)胞陽性檢測率,為日后臨床工作中對胃癌患者手術(shù)的方式選擇以及預(yù)后評估提供臨床佐證及參考,并初步探討對胃癌腹腔腫瘤脫落細(xì)胞檢測的臨床意義。方法:分別收集40例經(jīng)腹腔鏡下行胃癌D2根治術(shù)患者的術(shù)前、中腹腔沖洗液,35例經(jīng)開腹下行胃癌D2根治術(shù)患者的術(shù)前、中腹腔沖洗液。因流式細(xì)胞技術(shù)(flow cytometry,FCM)其一特性是利用DNA含量和倍體性判斷細(xì)胞良惡性,利用這一特性我們將其用來對腹腔沖洗液腫瘤脫落細(xì)胞進(jìn)行檢測,并將測定結(jié)果顯示DI≠1判定為檢測陽性。術(shù)后根據(jù)分組情況將所收集的標(biāo)本編號以對應(yīng)相應(yīng)病理檢查。結(jié)果:(1)在腹腔鏡組中手術(shù)前腹腔沖洗液腫瘤脫落細(xì)胞的檢測陽性率為20.00%(8/40)。而開腹組術(shù)前腫瘤腹腔沖洗液中檢脫落細(xì)胞的陽性率為17.14%(6/35),與腹腔鏡組無顯著差異(P0.05)。(2)腹腔鏡組術(shù)后腹腔沖洗液腫瘤脫落細(xì)胞的檢測陽性率為37.50%(15/40),與術(shù)前無顯著差異。開腹組腹腔沖洗液中檢測腫瘤脫落細(xì)胞的陽性率37.14%(13/35),與腹腔鏡組患者無顯著差異(P0.05)。結(jié)論:(1)腹腔鏡下和開腹下胃癌D2根治術(shù)患者腫瘤腹腔脫落細(xì)胞檢測陽性率無顯著差異,由于腹腔鏡相對開腹手術(shù)具有明顯的微創(chuàng)優(yōu)勢,在臨床工作上更適用于胃癌根治術(shù)。
[Abstract]:Objective: to detect tumor exfoliated cells in peritoneal lavage fluid of patients with gastric cancer by laparoscopy and laparotomy respectively by flow cytometry, and to compare the positive detection rate of tumor exfoliative cells between the two groups. To provide clinical evidence and reference for the selection of surgical methods and prognosis evaluation in patients with gastric cancer in the future, and to explore the clinical significance of detecting exfoliated cells in abdominal cancer of gastric cancer. Methods: 40 cases of gastric cancer D 2 radical operation under laparoscope and 35 cases of gastric cancer D 2 radical operation under laparoscopy were collected. One of the characteristics of flow cytometry (flow cytometry,FCM) is to use the content and ploidy of DNA to judge the benign and malignant cells, and we use it to detect the exfoliated cells in peritoneal lavage fluid. The results showed that DI 鈮，

本文編號：2210352