ATP生物熒光技術(shù)在口腔治療用水污染狀況現(xiàn)場快速檢測中的應(yīng)用初探
[Abstract]:Objective to investigate the feasibility of ATP bioluminescence technique for rapid detection of water pollution in oral treatment. Methods A total of 720 water samples were collected from the stomatology department of 48 medical institutions of the same size. According to the types of water supply in the comprehensive treatment table of stomatology department, they were divided into 4 groups. The total bacterial colony in the water was detected, and the ATP fluorescence value was detected with the special detection rod. The correlation between the two methods was analyzed and a mathematical model was established. The limit value of ATP fluorescence method was obtained when rapid detection was carried out in the field, and the water samples collected in the stomatology department of other medical institutions were analyzed to verify the feasibility of the test. Results there was significant correlation between ATP fluorescence method and laboratory plate counting method (rn 0.32 P 0.01). 4 groups of water samples were polluted in different water supply sources. 4 groups of data could fit the general linear equation of 0.869X-0.495 (Rhizoma Rhizoma 0.819P0.00). According to the standard of 100 CFU / mL for total bacteria in drinking water, the limit value of ATP fluorescence method (RLU) for field rapid detection is 787, respectively. 120 water samples collected in the stomatology department of other medical institutions in 2015 were analyzed with this limit. The overall sensitivity of the model was 83.87 and the specificity was 87.64 and the Kappa value was 0.673. Conclusion ATP fluorescence method can not completely replace the traditional microbial detection method, but it can be used as a rapid detection and screening method for the contamination of oral water in the field and improve the efficiency of supervision and law enforcement.
【作者單位】: 宜昌市衛(wèi)生計(jì)生綜合監(jiān)督執(zhí)法局;
【基金】:湖北省預(yù)防醫(yī)學(xué)會“消毒領(lǐng)域科研創(chuàng)新”項(xiàng)目(XKC2015-09)
【分類號】:R123;R115
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