【摘要】：目的：本研究擬通過(guò)探討低劑量X線照射(LDI)對(duì)大鼠骨髓來(lái)源的內(nèi)皮祖細(xì)胞功能的影響。 方法：取SD大鼠(體重200g)股骨、脛骨,反復(fù)沖洗骨髓腔,離心后取單核細(xì)胞細(xì),接種于25cm2的培養(yǎng)瓶中,置于37℃、體積分?jǐn)?shù)為0.05的二氧化碳、飽和濕度的培養(yǎng)箱中培養(yǎng)。使用流式細(xì)胞儀鑒定細(xì)胞后,實(shí)驗(yàn)組給予25mGy、50mGy、75mGy、100mGy低劑量X線及1Gy高劑量照射,對(duì)照組則不予照射。照射后分別于6h、1d、3d、5d、7d天計(jì)算細(xì)胞數(shù)及CCK-8檢測(cè)細(xì)胞增殖情況,照射后分別在第1d、3d、5d行Western-blot檢測(cè)細(xì)胞表面標(biāo)記：VEGFR-2;RT-PCR法測(cè)VEGFR-2mRNA的表達(dá)；免疫組織化學(xué)染色法測(cè)定細(xì)胞VWF、VEGFR-2的表達(dá)。 結(jié)果：低劑量照射后實(shí)驗(yàn)組較對(duì)照組的細(xì)胞計(jì)數(shù)有明顯增強(qiáng)；大劑量組可抑制細(xì)胞的增殖。CCK-8法檢測(cè)結(jié)果表明低劑量照射后,可明顯刺激內(nèi)皮祖細(xì)胞的增殖,與對(duì)照組相比有統(tǒng)計(jì)學(xué)意義(P0.05)。而1Gy高劑量照射則會(huì)抑制內(nèi)皮祖細(xì)胞增殖(P0.05)。實(shí)驗(yàn)組VEGFR-2在1d、3d、5d的表達(dá)高于對(duì)照組。而且隨著劑量的增加,表達(dá)量隨之升高(P0.05)。試驗(yàn)組VEGFR-2mRNA的表達(dá)也顯著強(qiáng)于對(duì)照組(P0.05)。免疫組織化學(xué)顯示在1和3天,實(shí)驗(yàn)組處的VWF和VEGFR-2表達(dá)強(qiáng)于對(duì)照組(P0.05)。 結(jié)論：低劑量X線照射后對(duì)內(nèi)皮祖細(xì)胞有興奮效應(yīng),表現(xiàn)為細(xì)胞生長(zhǎng)加速,同時(shí)在一定時(shí)間內(nèi)血管形成相關(guān)基因表達(dá)增強(qiáng)。
[Abstract]:Aim: to investigate the effects of low dose X-ray irradiation (LDI) on endothelial progenitor cells (EPCs) derived from rat bone marrow. Methods: the femur and tibia of SD rats (200g) were harvested, and the medullary cavity was washed repeatedly. After centrifugation, the monocytes were cultured in a culture bottle of 25cm2 and incubated in a incubator with saturated humidity at 37 鈩，

本文編號(hào)：2122859