肝星狀細胞中表皮形態(tài)發(fā)生素表達調(diào)控機制及其作用研究
發(fā)布時間：2019-01-10 08:41
【摘要】：肝星狀細胞是肝臟中重要的間質(zhì)細胞,是肝細胞外基質(zhì)的主要來源.表皮形態(tài)發(fā)生素(epimorphin、EPM、syntaxin2)在肝臟發(fā)育、再生及癌變過程中發(fā)揮了重要的作用,目前其表達變化的調(diào)控機制及對肝星狀細胞的作用還未有報道.通過對肝組織標(biāo)本進行檢測,發(fā)現(xiàn)肝纖維化過程中肝星狀細胞表達EPM上調(diào).從表觀遺傳學(xué)的角度對EPM表達變化調(diào)控機制進行研究,發(fā)現(xiàn)DNA去甲基化促進了EPM的表達.為了研究EPM對肝星狀細胞的可能的調(diào)節(jié)作用,將EPM表達質(zhì)粒轉(zhuǎn)染肝星狀細胞,之后檢測了EPM對肝星狀細胞增殖及遷移能力的變化.結(jié)果證明EPM能夠促進肝星狀細胞的增殖與遷移.本研究發(fā)現(xiàn),激活的肝星狀細胞高表達EPM可能是由于DNA去甲基化引起的,同時,高表達的EPM能夠促進肝星狀細胞的增殖與遷移,進而促進肝纖維化進展.
[Abstract]:Hepatic stellate cells are important mesenchymal cells in the liver and the main source of extracellular matrix. Epidermal morphogenetic hormone (epimorphin,EPM,syntaxin2) plays an important role in the development, regeneration and carcinogenesis of liver. The expression of EPM was up-regulated in hepatic stellate cells during hepatic fibrosis. From the perspective of epigenetics, the regulation mechanism of EPM expression change was studied. It was found that DNA demethylation promoted the expression of EPM. In order to study the possible regulatory effect of EPM on hepatic stellate cells, EPM expression plasmid was transfected into hepatic stellate cells, and then the changes of EPM on the proliferation and migration of hepatic stellate cells were detected. The results showed that EPM could promote the proliferation and migration of hepatic stellate cells. It was found that the overexpression of EPM in activated hepatic stellate cells may be due to the demethylation of DNA, and that the overexpression of EPM could promote the proliferation and migration of hepatic stellate cells and thus promote the progression of hepatic fibrosis.
【作者單位】： 解放軍醫(yī)學(xué)院;解放軍302醫(yī)院感染性疾病診療與研究中心;軍事醫(yī)學(xué)科學(xué)院野戰(zhàn)輸血研究所干細胞與再生醫(yī)學(xué)研究室;
【基金】：國家自然科學(xué)基金資助項目(81200303,81470097)~~
【分類號】：R575.2
[Abstract]:Hepatic stellate cells are important mesenchymal cells in the liver and the main source of extracellular matrix. Epidermal morphogenetic hormone (epimorphin,EPM,syntaxin2) plays an important role in the development, regeneration and carcinogenesis of liver. The expression of EPM was up-regulated in hepatic stellate cells during hepatic fibrosis. From the perspective of epigenetics, the regulation mechanism of EPM expression change was studied. It was found that DNA demethylation promoted the expression of EPM. In order to study the possible regulatory effect of EPM on hepatic stellate cells, EPM expression plasmid was transfected into hepatic stellate cells, and then the changes of EPM on the proliferation and migration of hepatic stellate cells were detected. The results showed that EPM could promote the proliferation and migration of hepatic stellate cells. It was found that the overexpression of EPM in activated hepatic stellate cells may be due to the demethylation of DNA, and that the overexpression of EPM could promote the proliferation and migration of hepatic stellate cells and thus promote the progression of hepatic fibrosis.
【作者單位】： 解放軍醫(yī)學(xué)院;解放軍302醫(yī)院感染性疾病診療與研究中心;軍事醫(yī)學(xué)科學(xué)院野戰(zhàn)輸血研究所干細胞與再生醫(yī)學(xué)研究室;
【基金】：國家自然科學(xué)基金資助項目(81200303,81470097)~~
【分類號】：R575.2
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