【摘要】：目的:探討幽門螺桿菌(Helicobacter pylori,H.pylori)作用巨噬細(xì)胞后導(dǎo)致的細(xì)胞炎癥反應(yīng)機制及對宿主巨噬細(xì)胞的損傷作用。方法:ELISA方法檢測幽門螺桿菌感染巨噬細(xì)胞后細(xì)胞培養(yǎng)上清液中細(xì)胞因子IL-23、IL-10、TNF-α及IL-8的含量,Western blot分析幽門螺桿菌作用巨噬細(xì)胞后細(xì)胞胞內(nèi)蛋白NOS2、COX2的表達(dá)水平;同時流式細(xì)胞技術(shù)分析幽門螺桿菌處理巨噬細(xì)胞后對細(xì)胞的凋亡作用。結(jié)果:細(xì)胞因子IL-23、IL-10、TNF-α、IL-8在幽門螺桿菌作用巨噬細(xì)胞后細(xì)胞培養(yǎng)上清中分泌顯著增多(P0.05),且NOS2、COX2蛋白表達(dá)顯著增強(P0.03);幽門螺桿菌處理巨噬細(xì)胞后巨噬細(xì)胞凋亡顯著增多(P0.05)。結(jié)論:幽門螺桿菌作用巨噬細(xì)胞后誘導(dǎo)巨噬細(xì)胞增強炎癥反應(yīng)而抑制或殺傷幽門螺桿菌,同時長時間作用能誘導(dǎo)宿主巨噬細(xì)胞凋亡。
[Abstract]:Aim: to investigate the mechanism of inflammatory reaction induced by Helicobacter pylori (Helicobacter pylori,H.pylori) on macrophages and its damage to host macrophages. Methods: the levels of cytokine IL-23,IL-10,TNF- 偽 and IL-8 in the supernatant of Helicobacter pylori infected macrophages were detected by ELISA method., Western blot analysis of intracellular protein NOS2, after Helicobacter pylori infection on macrophages was carried out. The expression level of COX2; At the same time, flow cytometry was used to analyze the apoptosis of macrophages treated with Helicobacter pylori. Results: the levels of cytokines IL-23,IL-10,TNF- 偽 and IL-8 increased significantly in the supernatant of macrophages after Helicobacter pylori treatment (P0.05), and the expression of NOS2,COX2 protein increased significantly (P0.03). After treated with Helicobacter pylori, macrophages apoptosis increased significantly (P0.05). Conclusion: Helicobacter pylori can induce macrophages to enhance inflammatory response and inhibit or kill Helicobacter pylori.
【作者單位】： 江蘇大學(xué)附屬昆山醫(yī)院檢驗科;重慶市腫瘤研究所病理科;
【基金】：昆山市科學(xué)與技術(shù)項目(No.KS1347) 江蘇大學(xué)醫(yī)學(xué)臨床科技發(fā)展基金項目(JYL-20140047)
【分類號】：R573

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