發(fā)布時(shí)間：2018-12-17 03:27

【摘要】：目的：氣道炎癥是涉及多種炎癥細(xì)胞和炎癥介質(zhì)的慢性免疫性疾病,目前對(duì)氣道炎癥的治療不理想,因此對(duì)其發(fā)病機(jī)制的進(jìn)一步研究以及尋找新的藥物治療、預(yù)防疾病是非常有意義的。CRF在外周炎癥中發(fā)揮重要的作用,本試驗(yàn)的目的是通過(guò)研究CRF在上氣道炎癥患者鼻組織中的表達(dá)情況以及與嗜酸性粒細(xì)胞、肥大細(xì)胞的相關(guān)性,探討CRF在氣道炎癥性疾病中的作用。方法：標(biāo)本均來(lái)自住院行鼻內(nèi)鏡手術(shù)的患者：變應(yīng)性鼻炎合并鼻息肉組10例、變應(yīng)性鼻炎組9例和正常對(duì)照組7例,鼻息肉組收集鼻息肉標(biāo)本,變應(yīng)性鼻炎組和正常對(duì)照組則收集下鼻甲標(biāo)本。行免疫組化染色和圖像分析,檢測(cè)CRF在組織中的表達(dá)情況；行HE染色、甲苯胺藍(lán)改良染色以計(jì)數(shù)嗜酸性粒細(xì)胞、肥大細(xì)胞；分析CRF在各組中的的表達(dá)以及與肥大細(xì)胞、嗜酸性粒細(xì)胞的相關(guān)性。結(jié)果：嗜酸性粒細(xì)胞、肥大細(xì)胞的數(shù)量在變應(yīng)性鼻炎組(13.90±3.08,10.22±2.96)及鼻息肉組(12.59±2.69,6.23±3.23)明顯高于對(duì)照組(7.33±4.18,4.48±2.38),差異具有統(tǒng)計(jì)學(xué)意義(P0.05)；CRF的光密度值在變應(yīng)性鼻炎組(0.273±0.028)及鼻息肉組(0.264±0.019)中明顯高于正常對(duì)照組(0.229±0.015),差異具有統(tǒng)計(jì)學(xué)意義(P0.01),然而CRF在變應(yīng)性鼻炎組和變應(yīng)性鼻炎合并鼻息肉組兩組間差異無(wú)顯著性；CRF在鼻部的表達(dá)與肥大細(xì)胞、嗜酸性粒細(xì)胞的數(shù)量均呈正相關(guān)(r分別為0.732,0.582)。結(jié)論：(1).CRF在鼻部粘膜表達(dá),可能參與了變應(yīng)性鼻炎與鼻息肉的鼻黏膜慢性炎癥反應(yīng)過(guò)程；(2).肥大細(xì)胞、嗜酸性粒細(xì)胞可能是鼻部CRF的主要來(lái)源；(3).CRF在上氣道炎癥性疾病中的表達(dá)及其與肥大細(xì)胞、嗜酸性粒細(xì)胞之間的聯(lián)系,為今后尋找新的治療方法提供了理論思路。
[Abstract]:Objective: airway inflammation is a chronic immune disease involving many inflammatory cells and inflammatory mediators. At present, the treatment of airway inflammation is not ideal. CRF plays an important role in peripheral inflammation. The purpose of this study was to investigate the expression of CRF in nasal tissues of patients with upper airway inflammation and its association with eosinophils and mast cells. To investigate the role of CRF in airway inflammatory diseases. Methods: the specimens were collected from 10 cases of allergic rhinitis complicated with nasal polyps, 9 cases of allergic rhinitis group and 7 cases of normal control group. Specimens of inferior turbinate were collected from allergic rhinitis group and normal control group. Immunohistochemical staining and image analysis were performed to detect the expression of CRF in tissues, HE staining and toluidine blue modified staining were performed to count eosinophils and mast cells. The expression of CRF in each group and its correlation with mast cells and eosinophils were analyzed. Results: the number of eosinophilic granulocytes and mast cells in allergic rhinitis group (13.90 鹵3.08 鹵10.22 鹵2.96) and nasal polyp group (12.59 鹵2.69 鹵6.23 鹵3.23) was significantly higher than that in control group (7.33 鹵4.184.48 鹵2.38). The difference was statistically significant (P0.05). The optical density of CRF in allergic rhinitis group (0.273 鹵0.028) and nasal polyp group (0.264 鹵0.019) was significantly higher than that in normal control group (0.229 鹵0.015). However, there was no significant difference in CRF between allergic rhinitis group and allergic rhinitis with nasal polyp group. The expression of CRF in the nose was positively correlated with the number of mast cells and eosinophils (r = 0.732 鹵0.582, respectively). Conclusion: (1) the expression of). CRF in nasal mucosa may be involved in the process of chronic inflammation of nasal mucosa in allergic rhinitis and nasal polyps; (2). Mast cells and eosinophilic granulocytes may be the main sources of nasal CRF. (3) the expression of). CRF in inflammatory diseases of upper airway and its relationship with mast cells and eosinophils, which provides a theoretical idea for finding new treatment methods in the future.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類(lèi)號(hào)】：R765

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