發(fā)布時(shí)間：2018-09-11 16:16

【摘要】：目的:探討腎活檢組織冰凍切片儲(chǔ)存溫度對(duì)免疫熒光結(jié)果的影響。方法:選擇2015年1月~2015年6月浙江省人民醫(yī)院腎臟病科腎臟活檢標(biāo)本100例,將同一腎穿組織同時(shí)切兩套切片分別儲(chǔ)存于-70℃低溫冰箱和-12℃冷凍切片機(jī)內(nèi),其中儲(chǔ)存于-70℃低溫冰箱的切片作為實(shí)驗(yàn)組,儲(chǔ)存于-12℃冷凍切片機(jī)內(nèi)的切片作為對(duì)照組,次日用直接免疫熒光法檢測免疫球蛋白(IgG、IgA和IgM)、補(bǔ)體(C3、C4和C1q)及纖維蛋白原,間接免疫熒光法檢測Ⅳ型膠原a3和a5鏈,熒光顯微鏡下觀察定性及半定量結(jié)果。結(jié)果:實(shí)驗(yàn)組3+~4+IgG 16例,對(duì)應(yīng)的對(duì)照組IgG均為1+~2+,陽性強(qiáng)度明顯弱于實(shí)驗(yàn)組,差異有統(tǒng)計(jì)學(xué)顯著性(P0.05);實(shí)驗(yàn)組3+~4+a3 99例,對(duì)照組1+~2+a3 92例,陰性8例;實(shí)驗(yàn)組3+~4+a5 99例,對(duì)照組1+~2+a5 92例,陰性8例,對(duì)照組陽性強(qiáng)度減弱,差異有統(tǒng)計(jì)學(xué)顯著性(P0.05)。實(shí)驗(yàn)組4+IgA 15例,3+IgA 19例,3+IgM 15例,2+IgM11例,4+C3 13例,3+C3 12例,相對(duì)應(yīng)的對(duì)照組陽性強(qiáng)度沒有明顯減弱,差異無統(tǒng)計(jì)學(xué)顯著性。結(jié)論:腎活檢冰凍切片免疫熒光結(jié)果受切片儲(chǔ)存溫度影響大,其中對(duì)IgG和Ⅳ型膠原免疫熒光陽性強(qiáng)度影響更大,建議腎臟活檢組織冰凍切片置于-70℃低溫冰箱儲(chǔ)存。
[Abstract]:Objective: to investigate the effect of storage temperature of frozen sections of renal biopsy tissue on immunofluorescence results. Methods: from January 2015 to June 2015, 100 renal biopsy specimens from the Department of Renal Disease, Zhejiang Provincial people's Hospital, were selected. Two sets of sections were cut into the same kidney tissue simultaneously and stored in -70 鈩，

本文編號(hào)：2237189