【摘要】：目的:探討腎活檢組織冰凍切片儲存溫度對免疫熒光結(jié)果的影響。方法:選擇2015年1月~2015年6月浙江省人民醫(yī)院腎臟病科腎臟活檢標(biāo)本100例,將同一腎穿組織同時切兩套切片分別儲存于-70℃低溫冰箱和-12℃冷凍切片機內(nèi),其中儲存于-70℃低溫冰箱的切片作為實驗組,儲存于-12℃冷凍切片機內(nèi)的切片作為對照組,次日用直接免疫熒光法檢測免疫球蛋白(IgG、IgA和IgM)、補體(C3、C4和C1q)及纖維蛋白原,間接免疫熒光法檢測Ⅳ型膠原a3和a5鏈,熒光顯微鏡下觀察定性及半定量結(jié)果。結(jié)果:實驗組3+~4+IgG 16例,對應(yīng)的對照組IgG均為1+~2+,陽性強度明顯弱于實驗組,差異有統(tǒng)計學(xué)顯著性(P0.05);實驗組3+~4+a3 99例,對照組1+~2+a3 92例,陰性8例;實驗組3+~4+a5 99例,對照組1+~2+a5 92例,陰性8例,對照組陽性強度減弱,差異有統(tǒng)計學(xué)顯著性(P0.05)。實驗組4+IgA 15例,3+IgA 19例,3+IgM 15例,2+IgM11例,4+C3 13例,3+C3 12例,相對應(yīng)的對照組陽性強度沒有明顯減弱,差異無統(tǒng)計學(xué)顯著性。結(jié)論:腎活檢冰凍切片免疫熒光結(jié)果受切片儲存溫度影響大,其中對IgG和Ⅳ型膠原免疫熒光陽性強度影響更大,建議腎臟活檢組織冰凍切片置于-70℃低溫冰箱儲存。
[Abstract]:Objective: to investigate the effect of storage temperature of frozen sections of renal biopsy tissue on immunofluorescence results. Methods: from January 2015 to June 2015, 100 renal biopsy specimens from the Department of Renal Disease, Zhejiang Provincial people's Hospital, were selected. Two sets of sections were cut into the same kidney tissue simultaneously and stored in -70 鈩，

本文編號：2237189