本文選題：鴨源雞桿菌　切入點(diǎn)：OmpA基因　出處：《河南農(nóng)業(yè)科學(xué)》2017年08期 　論文類型：期刊論文

【摘要】：為篩選鴨源雞桿菌(G.anatis)潛在的保護(hù)性抗原基因,參考Gen Bank中G.anatis UMN179的外膜蛋白A(OmpA)基因序列設(shè)計(jì)1對(duì)引物,對(duì)鴨源雞桿菌PDS-RZ-1-SLG(RZ)株的OmpA基因進(jìn)行克隆,并應(yīng)用相關(guān)軟件對(duì)其核苷酸及氨基酸序列進(jìn)行生物信息學(xué)分析。結(jié)果顯示,OmpA基因大小為1 083 bp,編碼360個(gè)氨基酸;鴨源雞桿菌RZ株的OmpA與UMN179、F149及12656/12的OmpA氨基酸相似性分別為93.2%、95.2%、92.7%;OmpA蛋白分子質(zhì)量為38.4 ku,等電點(diǎn)為6.77,具有穩(wěn)定性,N端有1個(gè)疏水性的α螺旋信號(hào)肽。
[Abstract]:In order to screen the potential protective antigen gene of G. anatisa, a pair of primers were designed to clone the OmpA gene of PDS-RZ-1-SLGG RZ strain from Gen Bank, referring to the gene sequence of G. anatis UMN179 outer membrane protein. The sequence of nucleotide and amino acid was analyzed by bioinformatics, and the result showed that the size of OmpA gene was 1 083 BP, encoding 360 amino acids. The similarity of OmpA amino acids between OmpA and UMN179 F149 and 12656/12 in RZ strain was 93.22.2 and 95.2%, respectively. The molecular weight of OmpA protein was 38.4 ku. the isoelectric point was 6.77. There was a hydrophobic 偽 helix signal peptide at the N terminal of RZ strain.
【作者單位】： 河南農(nóng)業(yè)大學(xué)牧醫(yī)工程學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(3177130375) 河南省高校科技創(chuàng)新團(tuán)隊(duì)與支持計(jì)劃資助項(xiàng)目(14IRTSHN015)
【分類號(hào)】：S852.61
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本文編號(hào)：1607469