本文選題：隱孢子蟲 + PCR　； 參考：《鄭州大學(xué)》2006年碩士論文

【摘要】：背景和目的 隱孢子蟲病(Cryptosporidiosis)是一種全球性的人獸共患寄生蟲病，有著重要公共衛(wèi)生意義。隱孢子蟲可寄生于禽類、哺乳類、爬行類和兩棲類等170種以上的人和動物。在人類患者主要引起腹瀉和呼吸道癥狀，艾滋病病人和兒童中的感染率分別高達(dá)48％和17.5％，可導(dǎo)致嚴(yán)重的水樣腹瀉甚至危及生命，免疫功能正常者也能感染出現(xiàn)急性自限性腹瀉。該病已被列為引起人類最常見的6種腹瀉疾病之一。 本文對河南地區(qū)3株人源隱孢子蟲分離株進(jìn)行了形態(tài)學(xué)測定、動物交叉感染試驗、致病性和組織病理研究，并進(jìn)行了PCR和基因進(jìn)化樹的構(gòu)建，來確定3個隱孢子蟲分離株的生物學(xué)特性。 對于隱孢子蟲病的深入研究，有賴于其動物模型的建立。3個隱孢子蟲分離株均能成功感染免疫力低下及免疫力正常的小白鼠。在3個分離株中，1號和2號分離株感染小白鼠后出現(xiàn)的臨床癥狀和病理變化基本一致，，且明顯嚴(yán)重于3號分離株對小鼠的感染。此外1號和2號分離株感染小白鼠后的排隱孢子蟲卵囊規(guī)律幾乎一致，但二者與3號分離株感染小白鼠后的排隱孢子蟲卵囊規(guī)律差別較大。在寄生部位上3者也有明顯的區(qū)別，1號、2號分離株主要寄生于小白鼠的回腸，3號分離株主要寄生于小白鼠的十二指腸。只有3號分離株能夠成功感染雛雞，并且3號分離株能夠使試驗雛雞出現(xiàn)嚴(yán)重的臨床癥狀和病理變化。在寄生部位上，3號分離株主要寄生于雛雞的回腸，這明顯區(qū)別于感染小白鼠時的十二指腸。結(jié)果表明1、2號分離株為同一個種屬，3號分離株為一個種屬。
[Abstract]:Background and objective Cryptosporidiosism (Cryptosporidiosism) is a global zoonotic parasitic disease, which has important public health significance. Cryptosporidium can parasitize more than 170 species of people and animals, such as birds, mammals, reptiles and amphibians. In human patients, the main causes of diarrhea and respiratory symptoms, AIDS patients and children with infection rates as high as 48% and 17.5%, can lead to serious watery diarrhea and even life-threatening. People with normal immune function can also be infected with acute self-limited diarrhea. The disease has been listed as one of the six most common diarrhoeal diseases. In this paper, three human Cryptosporidium isolates in Henan Province were studied by morphological analysis, animal cross infection test, pathogenicity and histopathology, and the construction of PCR and gene evolution tree. To determine the biological characteristics of three Cryptosporidium isolates. The further study of Cryptosporidiosis depends on the establishment of animal models. All three Cryptosporidium isolates can successfully infect mice with low immunity and normal immunity. The clinical symptoms and pathological changes of mice infected with 1 and 2 strains were basically consistent, and were significantly more serious than the infection of strain 3 in mice. In addition, the oocysts of Cryptosporidium oocysts infected by isolates 1 and 2 were almost the same, but the rules of oocysts of Cryptosporidium were different from those of strain 3. There were also significant differences in the parasitic sites of the 3 isolates. Isolates 1 and 2 were mainly parasitic on the ileum of the mice and isolates 3 were mainly parasitic on the duodenum of the mice. Only strain 3 could infect chicks successfully, and strain 3 could cause serious clinical symptoms and pathological changes in experimental chicks. In the parasitic site, isolate 3 was mainly parasitic on the ileum of the chicks, which was obviously different from the duodenum of the infected mice. The results showed that strain 1 and 2 belonged to the same genus, and strain 3 belonged to one genus.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R382

【參考文獻(xiàn)】

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1 賈世英,孫金閣;隱孢子蟲病的流行病學(xué)、診斷與防治[J];山東醫(yī)藥;2003年19期

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