發(fā)布時(shí)間：2018-02-25 05:34

  本文關(guān)鍵詞： 腸道病毒 手足口病 VP基因 同源性分析　出處：《病毒學(xué)報(bào)》2014年06期 　論文類型：期刊論文

【摘要】：了解三門峽市2011年手足口病例及健康兒童腸道病毒型別及生物學(xué)特征。本研究采集55份手足口病臨床病例及60份健康兒童糞便接種敏感細(xì)胞進(jìn)行腸道病毒分離,提取RNA,用熒光RT-PCR檢測(cè)腸道病毒通用型(Pan-enterovirus,PE)、腸道病毒71型(Human enterovirus 71,EV71)、柯薩奇病毒A16型(Coxsackie virus A16,CA16)。EV71、CA16分別用各自的VP1引物全序測(cè)定鑒定,對(duì)其它腸道病毒使用引物對(duì)012/011、008/013進(jìn)行擴(kuò)增測(cè)序,并與已知序列對(duì)比分型鑒定,并且對(duì)EV71病毒VP1基因特征進(jìn)行了分析。臨床病例腸道病毒檢出率為52.73%(29/55)、健康兒童腸道病毒檢出率為18.33%(11/60);臨床病例檢出22株EV71、4株CA16和3株其它腸道病毒,健康兒童檢出腸道病毒11株,其中柯薩奇B組病毒占81.82%(9/11)。對(duì)19株EV71病毒VP1基因分析證實(shí),三門峽市2011年流行株為C4a亞群,其進(jìn)化聚類以靈寶市、盧氏縣聚類最為明顯。引起手足口病主要為EV71和CA16,健康兒童中存在大量的柯薩奇B組病毒,隱性感染廣泛存在,2011年本地EV71病毒流行株為C4a亞群,有明顯地域聚集特點(diǎn)。
[Abstract]:To understand the case of hand, foot and mouth disease (HFMD) in Sanmenxia city in 2011 and the types and biological characteristics of enterovirus in healthy children. In this study, 55 clinical cases of HFMD and 60 fecal inoculation sensitive cells of healthy children were collected for enterovirus isolation. RNAs were extracted and detected by fluorescent RT-PCR for the common type of enterovirus Pan-enterovirus.Enterovirus 71 human enterovirus 71 and Coxsackie virus A16CA16.EV71 for Coxsackie virus A16CA16.EV71mCA16, respectively, were amplified and sequenced using primers from other enteroviruses, 012r011n008013, respectively. The results showed that the detection rate of enterovirus in clinical cases was 52.7329 / 55, the positive rate of enterovirus in healthy children was 18.33 / 110.22 strains of CA16 and 3 other enterovirus strains were detected in clinical cases. Eleven strains of enterovirus were detected in healthy children, of which Coxsackie B virus accounted for 81.82% of the 11 strains. The analysis of VP1 gene of 19 strains of EV71 virus confirmed that the prevalent strain in Sanmenxia city in 2011 was C4a subgroup, and its evolutionary cluster was Lingbao city. The main causes of HFMD were EV71 and CA16.The coxsackie B virus (Coxsackievirus B) was found in healthy children, and recessive infection was widespread. In 2011, the endemic strain of EV71 virus was C4a subgroup, which had obvious regional aggregation characteristics.
【作者單位】： 河南省三門峽市疾病預(yù)防控制中心;河南省疾病預(yù)防控制中心傳染病預(yù)防控制所;
【基金】：河南省科技攻關(guān)計(jì)劃項(xiàng)目(122102310268) 河南省醫(yī)學(xué)科技重大攻關(guān)項(xiàng)目(201001015) 河南省衛(wèi)生科技創(chuàng)新型人才工程中青年科技創(chuàng)新人才
【分類號(hào)】：R373.2

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本文編號(hào)：1533229