本文關(guān)鍵詞： 骨髓間充質(zhì)細(xì)胞 海綿體平滑肌細(xì)胞 細(xì)胞培養(yǎng) 誘導(dǎo)　出處：《華中科技大學(xué)》2007年碩士論文　論文類型：學(xué)位論文

【摘要】： 目的: 觀察在海綿體平滑肌細(xì)胞提取物誘導(dǎo)下,大鼠骨髓間充質(zhì)干細(xì)胞(MSCs)向大鼠海綿體平滑肌細(xì)胞(CSMCs)轉(zhuǎn)化的可能。 方法: 取4～5周左右SD大鼠,斷頸處死后無菌條件下分離出股骨、脛骨。剪斷干骺端后用5ml注射器吸取培養(yǎng)基沖洗骨髓腔,將液體直接沖入離心管中,用密度梯度離心法從沖洗出的細(xì)胞中分離出MSCs,進(jìn)行原代培養(yǎng)。兩代后,用不同數(shù)量CSMCs裂解獲取的細(xì)胞提取物(cell extract, CE)進(jìn)行誘導(dǎo),24h后分別進(jìn)行免疫組化分析并觀察其形態(tài)學(xué)特性。 結(jié)果: 海綿體平滑肌細(xì)胞CE誘導(dǎo)的細(xì)胞均可表達(dá)α-actin,且高倍鏡下觀察可見部分海綿體平滑肌細(xì)胞特征,對照組中未見α-actin的表達(dá),高倍鏡下觀察也無海綿體平滑肌細(xì)胞特征。CE組和對照組間有顯著性差異,各CE組之間也有差異。即在海綿體平滑肌細(xì)胞CE誘導(dǎo)下,MSCs可以轉(zhuǎn)化為CSMCs。 結(jié)論: MSCs在海綿體平滑肌細(xì)胞CE誘導(dǎo)下可以分化為CSMCs。
[Abstract]:Objective:. To observe the possible transformation of rat bone marrow mesenchymal stem cells (MSCs) into rat cavernous smooth muscle cells (CSMCs) induced by extractant from cavernous smooth muscle cells (SMC). Methods:. The femur and tibia were isolated from SD rats about 5 weeks after cervical dissection. After cutting off the metaphysis, the medullary cavity was washed with a 5 ml syringe and the liquid was washed directly into the centrifuge tube. MSCs were isolated from the washed cells by density gradient centrifugation and cultured in primary culture. After two generations, the cells were induced by different numbers of CSMCs cleavage cells (CES) for 24 hours and their morphological characteristics were analyzed by immunohistochemistry. Results:. Cavernous smooth muscle cells induced by CE could express 偽 -actinin. Some features of smooth muscle cells of cavernous body were observed under high power microscope, but no expression of 偽 -actin was found in control group. There was also no significant difference between CE group and control group under high power microscope, that is, MSCs could be transformed into CSMCsunder CE induced by cavernous smooth muscle cells. Conclusion:. MSCs can differentiate into CSMCs under CE induction of cavernous smooth muscle cells.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R329

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 劉波;劉繼紅;;海綿體平滑肌細(xì)胞的體外培養(yǎng)的研究[J];中國男科學(xué)雜志;2006年05期

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