發(fā)布時(shí)間：2018-02-13 15:34

  本文關(guān)鍵詞： 間充質(zhì)干細(xì)胞 T淋巴細(xì)胞 TGF-β_1 HGF GVHD　出處：《鄭州大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

【摘要】：背景與目的： 間充質(zhì)干細(xì)胞(Mesenchymal stem cells，MSCs)是骨髓非造血細(xì)胞中成體干細(xì)胞中的一種，具有很強(qiáng)的自我更新能力和多向分化潛能。在體外不同分化條件的誘導(dǎo)下，可以形成成骨細(xì)胞、軟骨細(xì)胞或脂肪細(xì)胞等，并且克隆后的細(xì)胞具有類似分化潛能，充分證明了間充質(zhì)干細(xì)胞是多能分化細(xì)胞。除在骨髓中發(fā)現(xiàn)間充質(zhì)干細(xì)胞外，在骨骼肌、心肌、真皮，以及腦和外周血中，也發(fā)現(xiàn)有類似骨髓間充質(zhì)干細(xì)胞(BM-MSCs)生物學(xué)特性和表面標(biāo)記的間充質(zhì)干細(xì)胞。間充質(zhì)干細(xì)胞表型不均一，，分別具有間充質(zhì)細(xì)胞、上皮細(xì)胞和內(nèi)皮細(xì)胞的特點(diǎn)。對(duì)人體外擴(kuò)增的BM—MSCs的研究結(jié)果顯示一致表達(dá)SH2、SH3、CD29、CD44、CD77、CD90、CD106、CD120a、CD124等抗原，不表達(dá)造血干細(xì)胞(HSC)的標(biāo)志性抗原CD14、CD34、CD45。但是目前還沒有特異的表面標(biāo)記來證實(shí)間充質(zhì)干細(xì)胞。動(dòng)物實(shí)驗(yàn)和臨床研究均發(fā)現(xiàn)，異基因造血干細(xì)胞移植時(shí)共同輸入供體間充質(zhì)干細(xì)胞可以降低移植失敗率并減輕移植物抗宿主病(GVHD)。具體機(jī)制尚不明確，大多數(shù)學(xué)者認(rèn)為間充質(zhì)干細(xì)胞可能通過抑制T淋巴細(xì)胞增殖來減輕移植后GVHD，而這種抑制作用是通過分泌可溶性細(xì)胞因子實(shí)現(xiàn)的，最受關(guān)注的細(xì)胞因子是轉(zhuǎn)化生長(zhǎng)因子-β_1(TGF-β_1)和肝細(xì)胞生長(zhǎng)因子(HGF)。 本實(shí)驗(yàn)通過體外培養(yǎng)間充質(zhì)干細(xì)胞(Mesenchymal stem cells，MSCs)探索其鑒定方法，觀察間充質(zhì)干細(xì)胞的生物學(xué)特性及TGF-β_1和HGF的表達(dá)，通過與T淋巴細(xì)胞的混合培養(yǎng)研究其抑制T淋巴細(xì)胞增殖，減輕GVHD的機(jī)制。 材料與方法：
[Abstract]:Background and purpose:. Mesenchymal stem cells (MSCs) is one of the adult stem cells in bone marrow non-hematopoietic cells. Chondrocytes, adipocytes, etc., and the cloned cells have similar differentiation potential, which fully proves that mesenchymal stem cells are pluripotent cells, except mesenchymal stem cells found in bone marrow, skeletal muscle, myocardium, dermis, And in the brain and peripheral blood, we also found BM-MSCslike mesenchymal stem cells with different phenotypes, and mesenchymal stem cells with mesenchymal cells. The characteristics of epithelial cells and endothelial cells. The results of BM-MSCs amplification in human body showed that the antigens SH2OSH3ONCD29, CD44, CD770, CD90, CD106, CD120a, CD124, and so on, were expressed in a consistent manner. But there is no specific surface marker to confirm mesenchymal stem cells. Animal experiments and clinical studies have found that, Allogeneic hematopoietic stem cell transplantation combined with donor mesenchymal stem cells can reduce graft failure rate and alleviate graft versus host disease (GV HDD). The mechanism is not clear. Most researchers believe that mesenchymal stem cells may reduce GVHD after transplantation by inhibiting T lymphocyte proliferation, which is achieved by secreting soluble cytokines. The most concerned cytokines were transforming growth factor- 尾 (TGF- 尾) and hepatocyte growth factor (HGF- 尾). In this experiment, mesenchymal stem cells were cultured in vitro to explore the identification of mesenchymal stem cells. The biological characteristics of mesenchymal stem cells and the expression of TGF- 尾 1 and HGF were observed. The proliferation of T lymphocytes was inhibited by co-culture with T lymphocytes. The mechanism of alleviating GVHD. Materials and methods:
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R392

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