本文關(guān)鍵詞：烏司他丁和胸腺肽α1聯(lián)合治療嚴重膿毒癥的組織病理學觀察，由筆耕文化傳播整理發(fā)布。

網(wǎng)友1557281760近日為您收集整理了關(guān)于烏司他丁和胸腺肽α1聯(lián)合治療嚴重膿毒癥的組織病理學觀察的文檔，希望對您的工作和學習有所幫助。以下是文檔介紹：·830 · 南方醫(yī)科大學學報(J South Med Univ) 2012;32(6) 烏司他丁和胸腺肽0c 1聯(lián)合治療嚴重膿毒癥的組織病理學觀察郭劍穎,鄧群,郭旭升,劉雙慶,張玉紅,何忠杰,林洪遠解放軍總醫(yī)院第一附屬醫(yī)院ICU,北京 100048 摘要:目的通過組織病理學觀察聯(lián)合應(yīng)用烏司他丁和胸腺肽c【1治療嚴重膿毒癥的效果。方法采用經(jīng)典大鼠CLP膿毒癥模型, 全部動物隨機分為對照組、聯(lián)合治療組(烏司他丁+胸腺肽Ⅱ1)、烏司他丁治療組和胸腺肽a1治療組。制模成功后分別于6、24、48 和72h經(jīng)尾靜脈或皮下注射給藥,記錄每天死亡數(shù)。制模后24、48、72和96h處死動物,留取心、肝、脾、肺、腎、小腸標本,以光鏡進行病理學檢查,脾臟標本行凋亡檢測。結(jié)果聯(lián)合治療組死亡率顯著降低(P=O.0325);組織病理學顯示,對照組各臟器損害最嚴重,單藥組其次,聯(lián)合用藥組最輕;此外,對照組脾臟細胞大量凋亡,聯(lián)合用藥組凋亡程度顯著下降[(47.4￣10.9)% (39.3士 11.4)%,P=O.0000]。結(jié)論烏司他丁和胸腺肽Ⅱ1聯(lián)合用藥可以顯著減輕膿毒癥大鼠臟器損害,抑制脾臟細胞凋亡,提高膿毒癥大鼠存活率。關(guān)鍵詞:膿毒癥/病理學;烏司他丁;胸腺肽 1;聯(lián)合用藥中圖分類號:R631.2 文獻標志碼:A 文章編號:1673.4254(2012)06.0830.05 doi:10.3969/j.issn.1673-4254.2012.06.016 s/detail/44.1627.R.20120601.1712.006.html Ulinastatin com bined with thym osin al for treatment of severe sepsis: a hist0path0l0gical observation in rats GUO[ianying,DENG Qun,GUO Xusheng,LIU Shuangqing,ZHANG Yuhong,HE Zhongjie,LIN Hongyuan Intensive Care Unit,First Affiliated Hospital ol General Hospital oiPLA Beijing 100048|China Abstract:Objective To evaluate the therapeutic effect of bined with thymosin al in the treatment of severe sepsis in rats.Methods Normal Wistar rats were subject to cecal ligation and puncture(CLP)to establish models of severe sepsis.The rats were then randomized into 4 groups for treatment with saline(contro1),ulinastatin,thymosin al,or bination of the latter two injected throu gh the caudal vein or subcutaqneously at 6,24,48 and 72 h after modeling.The mortality rate was recorded daily and the rats were executed at 24,48,72 and 96 h after CLP to harvest the heart,liver,spleen, lung,kidney and small in testines for pathological examination.The spleen of the rats were taken for detection of apoptosis of the spleen cells.Results Th e mortality rate of the septic rats in bined treatment group was decreased significantly fP= O.0325).The control group showed the most an damage,w_hich was moderate in single drug treatment group and the m ildest bined treatment group.Obvious spleen cell apoptosis was found in the control group,and was significantly ameliorated in bined treatment group【(47.4_+10.9)% VS(39.3-+11.4)%,P=O.0000].bined treatment with ulinastatin and thym osin al can sign ificantly improve the prognosis and an damage and spleen cell apoptosis in rats w ith sever sepsis. Key words:sepsis/pathology;ulinastatin;thymosin bination therapy 嚴重膿毒癥是危重患者面對的最嚴重威脅之一,其死亡率高、療效差,也是目前醫(yī)學領(lǐng)域的重大難題n]。隨著研究的不斷深人,人們逐漸認識到免疫紊亂是導致膿毒癥患者死亡的重要因素。2007年“膿毒癥免疫調(diào)理治療協(xié)作組”報告,聯(lián)合采用烏司他丁和胸腺肽a l的免疫調(diào)理治療策略能夠明顯改善嚴重膿毒癥病人預(yù)后,取得該領(lǐng)域治療研究的重大突破嘲。但由于臨床的局限I生, 使該研究缺乏病理學資料,本研究試圖通過實驗病理學收稿日期:2012—04 18 基金項目:北京市科技計劃重大項目作者簡介:郭劍穎,主治醫(yī)師,在讀博士研究生,E-maik guojy2003@ yahoo.corn .Cll 通訊作者:鄧群,博士,副主任醫(yī)師,E.mail:vincentdeng036@hotmail. CO m 研究進一步驗證抗炎與免疫刺激聯(lián)合治療的合理}生。 1材料和方法 1.1實驗動物清潔級健康成年雄性 Wistar大鼠 144只,體質(zhì)量 230~270 g,由軍事醫(yī)學科學院動物中心提供。 1.2主要試劑與儀器大鼠凋亡檢測試劑盒 In situ cell death detection Kit(Roche,Germany)。Olympus BX.50光學顯微鏡(日本Olympus公司),CMIAS系列多功能真彩色(JL京航空航天大學)病理圖像分析系統(tǒng)。 1.3 at4-癥動物模型制備動物購人后適應(yīng)性飼養(yǎng) 1周。實驗

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  本文關(guān)鍵詞：烏司他丁和胸腺肽α1聯(lián)合治療嚴重膿毒癥的組織病理學觀察，由筆耕文化傳播整理發(fā)布。