【摘要】：本論文旨在研發(fā)一款針對體力疲勞有輔助保護(hù)作用的保健食品,研究以人參、鹿胎、玫瑰花、黃精為原料,以白砂糖為輔料制成的保健葡萄酒。對提取工藝進(jìn)行考察,分別采用滲濾法、熱回流法、冷浸法制備人參鹿胎抗疲勞葡萄酒,以總皂苷的含量以及葡萄酒的感官評價為指標(biāo)綜合評價,篩選出最佳制備方法。以料液質(zhì)量比、提取時間、提取次數(shù)為因素設(shè)計正交試驗,以上述指標(biāo)為依據(jù),優(yōu)選出最佳工藝條件為采用冷浸法,正交實驗最佳條件為料液質(zhì)量比1:10、每次冷浸1天、冷浸2次。對制劑成型工藝的考察,分別考察離心轉(zhuǎn)速、離心時間對樣品產(chǎn)生沉淀的影響,確定最佳的離心條件確定最佳的離心條件為4000 r/min時間為6 min。多個樣品中分別加入一定量的白砂糖或蜂蜜,根據(jù)感官評價得到最宜加入白砂糖,加入量為3g/100mL。對產(chǎn)品進(jìn)行質(zhì)量標(biāo)準(zhǔn)研究,考察紫外分光光度法測定人參鹿胎抗疲勞葡萄酒中總皂苷的含量的精密性、可重復(fù)性和加標(biāo)回收率確定其線性范圍,驗證了該方法穩(wěn)定、可行。對人參鹿胎抗疲勞葡萄酒進(jìn)行功能學(xué)評價,各劑量組血清尿素氮含量與對照組比較均有降低,高劑量組與空白組的差別統(tǒng)計學(xué)意義,能夠提升小鼠肝糖原儲備能力,增加小鼠游泳時長,高劑量組與空白組差別有統(tǒng)計學(xué)意義;游泳后曲線下面積雖與對照組比較無統(tǒng)計學(xué)意義,但有下降的趨勢,可見人參鹿胎抗疲勞葡萄酒具有緩解體力疲勞的功效。對人參鹿胎抗疲勞葡萄酒進(jìn)行毒理學(xué)評價,急性毒性試驗中MTD高于21 g/kg BW,屬于無毒級;微核試驗、Ames試驗、精子畸形試驗結(jié)果均為陰性;三十天喂養(yǎng)試驗每項指標(biāo)都沒有發(fā)現(xiàn)不良影響。可見人參鹿胎抗疲勞葡萄酒安全可靠。
[Abstract]:The purpose of this paper is to develop a kind of health food which can protect physical fatigue, and study the health care wine made from ginseng, deer fetus, rose and yellow essence, and white sugar as auxiliary material. The extraction process was investigated and the best preparation method was obtained by synthetically evaluating the content of total saponins and the sensory evaluation of the wine by using percolation method heat reflux method and cold soaking method to prepare Panax ginseng deer fetal anti-fatigue wine with the content of total saponins as well as the sensory evaluation of the wine. The orthogonal experiment was designed with the mass ratio of material to liquid, extraction time and times of extraction as the factors. According to the above indexes, the optimum technological conditions were as follows: using cold leaching method. The optimum condition of orthogonal experiment was that the mass ratio of material to liquid was 1: 10, and the cold soaking was 1 day each time. Cold dip twice. The effects of centrifugal speed and time on the precipitation of the sample were investigated. The optimum centrifugation conditions were determined as 4000 r/min and 6 min.. A certain amount of white sugar or honey was added to several samples. According to the sensory evaluation, the most suitable addition of white sugar was 3 g / 100 mL. The quality standard of the product was studied. The precision of the determination of total saponins in ginseng deer fetal antifatigue wine by ultraviolet spectrophotometry was investigated. The repeatability and the recovery rate were determined. The method was proved to be stable and feasible. The functional evaluation of ginseng deer fetal antifatigue wine showed that the serum urea nitrogen content in each dose group was lower than that in the control group, and the difference between the high dose group and the blank group was statistically significant, which could improve the liver glycogen reserve ability of mice. Increase the swimming time of mice, the difference between the high dose group and the blank group was statistically significant; The area under the curve after swimming had no statistical significance compared with the control group, but it had a downward trend. It can be seen that ginseng deer fetal antifatigue wine has the effect of relieving physical fatigue. The toxicological evaluation of ginseng deer fetal antifatigue wine showed that MTD was higher than 21 g/kg BW, in acute toxicity test, but the results of micronucleus test, Ames test and sperm malformation test were all negative. No adverse effects were found in each index of the 30-day feeding trial. It can be seen that ginseng deer fetal anti-fatigue wine is safe and reliable.
【學(xué)位授予單位】：長春工業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TS262.6

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