超高效液相色譜-串聯(lián)質(zhì)譜法檢測(cè)魚肉和暫養(yǎng)水中丁香酚殘留量
發(fā)布時(shí)間：2018-09-10 17:55
【摘要】：建立超高效液相色譜-串聯(lián)質(zhì)譜測(cè)定魚肉和暫養(yǎng)水中丁香酚殘留量的分析方法。魚肉樣品經(jīng)正己烷提取,水樣則直接用HLB小柱濃縮、富集與凈化,以電噴霧離子源負(fù)離子模式,在多反應(yīng)監(jiān)測(cè)模式下測(cè)定,內(nèi)標(biāo)法定量。結(jié)果表明,樣品在4 min內(nèi)完成測(cè)定,在1~200μg/L質(zhì)量濃度范圍內(nèi),相關(guān)系數(shù)(R~2)大于0.996,魚肉樣品的加標(biāo)回收率在88.6%~106%之間,相對(duì)標(biāo)準(zhǔn)偏差為7.82%~11.90%;水樣的加標(biāo)回收率在89.2%~96.5%之間,相對(duì)標(biāo)準(zhǔn)偏差為5.18%~7.83%。魚肉和水樣中丁香酚的定量限分別為2.5μg/kg和0.05μg/L。
[Abstract]:A method for the determination of eugenol residues in fish meat and temporary aquaculture water by ultra-high performance liquid chromatography-tandem mass spectrometry was developed.The fish meat samples were extracted by n-hexane and the water samples were concentrated and purified by HLB column.The samples were determined by electrospray ionization under multi-reaction monitoring mode and quantified by internal standard method. The relative standard deviation was 7.82%~11.90%. The relative standard deviation was between 89.2%~96.5%. The relative standard deviation was 5.18%~7.83%. The quantitative limits of eugenol in fish and water samples were 2.5 ug/kg and 2.5 ug/kg, respectively. 0.05 mu g/L.
【作者單位】： 中國(guó)水產(chǎn)科學(xué)研究院南海水產(chǎn)研究所農(nóng)業(yè)部水產(chǎn)品加工重點(diǎn)實(shí)驗(yàn)室農(nóng)業(yè)部水產(chǎn)品貯藏保鮮質(zhì)量安全風(fēng)險(xiǎn)評(píng)估實(shí)驗(yàn)室(廣州)農(nóng)業(yè)部漁業(yè)環(huán)境及水產(chǎn)品質(zhì)量監(jiān)督檢驗(yàn)測(cè)試中心(廣州);
【基金】：中國(guó)水產(chǎn)科學(xué)研究院南海水產(chǎn)研究所基本科研業(yè)務(wù)費(fèi)項(xiàng)目(2014TS28) 廣東省水產(chǎn)品質(zhì)量安全專項(xiàng)(GDSCZA2015008) 國(guó)家農(nóng)產(chǎn)品質(zhì)量安全風(fēng)險(xiǎn)評(píng)估重大專項(xiàng)(GJFP201501003)
【分類號(hào)】：TS254.7;O657.63
本文編號(hào)：2235183
[Abstract]:A method for the determination of eugenol residues in fish meat and temporary aquaculture water by ultra-high performance liquid chromatography-tandem mass spectrometry was developed.The fish meat samples were extracted by n-hexane and the water samples were concentrated and purified by HLB column.The samples were determined by electrospray ionization under multi-reaction monitoring mode and quantified by internal standard method. The relative standard deviation was 7.82%~11.90%. The relative standard deviation was between 89.2%~96.5%. The relative standard deviation was 5.18%~7.83%. The quantitative limits of eugenol in fish and water samples were 2.5 ug/kg and 2.5 ug/kg, respectively. 0.05 mu g/L.
【作者單位】： 中國(guó)水產(chǎn)科學(xué)研究院南海水產(chǎn)研究所農(nóng)業(yè)部水產(chǎn)品加工重點(diǎn)實(shí)驗(yàn)室農(nóng)業(yè)部水產(chǎn)品貯藏保鮮質(zhì)量安全風(fēng)險(xiǎn)評(píng)估實(shí)驗(yàn)室(廣州)農(nóng)業(yè)部漁業(yè)環(huán)境及水產(chǎn)品質(zhì)量監(jiān)督檢驗(yàn)測(cè)試中心(廣州);
【基金】：中國(guó)水產(chǎn)科學(xué)研究院南海水產(chǎn)研究所基本科研業(yè)務(wù)費(fèi)項(xiàng)目(2014TS28) 廣東省水產(chǎn)品質(zhì)量安全專項(xiàng)(GDSCZA2015008) 國(guó)家農(nóng)產(chǎn)品質(zhì)量安全風(fēng)險(xiǎn)評(píng)估重大專項(xiàng)(GJFP201501003)
【分類號(hào)】：TS254.7;O657.63
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