發(fā)布時間：2018-09-08 08:03

【摘要】：制備了銅綠假單胞菌SJTD-1菌株的蛋白質(zhì)組酶解產(chǎn)物,并利用二氧化鈦磁珠從酶解產(chǎn)物中富集磷酸化肽.首次利用納升液相色譜-質(zhì)譜技術(shù)鑒定磷酸化肽的磷酸化位點(diǎn),最終共鑒定獲得13條磷酸化肽,分別來自12個磷酸化蛋白,其中有7個磷酸化蛋白在假單胞菌中是首次發(fā)現(xiàn).分析鑒定到的磷酸化蛋白,發(fā)現(xiàn)大多與DNA復(fù)制、物質(zhì)轉(zhuǎn)運(yùn)、能量代謝等密切相關(guān).這是首次對銅綠假單胞菌SJTD-1菌株進(jìn)行磷酸化蛋白質(zhì)組學(xué)分析,為SJTD-1在DNA復(fù)制、物質(zhì)和能量代謝以及細(xì)胞膜轉(zhuǎn)運(yùn)等方面的研究奠定基礎(chǔ).
[Abstract]:The proteomic hydrolysis products of Pseudomonas aeruginosa (SJTD-1) strain were prepared and the phosphorylated peptides were enriched by titanium dioxide magnetic beads. The phosphorylation sites of phosphorylated peptides were identified by nanolith liquid chromatography-mass spectrometry for the first time. A total of 13 phosphorylated peptides were identified, each from 12 phosphorylated proteins, of which 7 phosphorylated proteins were first found in Pseudomonas. Most of the phosphorylated proteins identified were closely related to DNA replication, substance transport and energy metabolism. This is the first time that the phosphorylation proteomics analysis of Pseudomonas aeruginosa SJTD-1 strain is carried out, which lays a foundation for the study of SJTD-1 in DNA replication, substance and energy metabolism and cell membrane transport.
【作者單位】： 上海交通大學(xué)藥學(xué)院;上海交通大學(xué)生命科學(xué)與技術(shù)學(xué)院;上海交通大學(xué)分析測試中心;
【基金】：國家自然科學(xué)基金項(xiàng)目(31200062)資助
【分類號】：O629.73;O657.63

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