本文選題：羅丹明 + pH探針��； 參考：《西南醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:pH值在各種系統(tǒng)中起著舉足輕重的作用,因此對(duì)pH值的檢測(cè)是至關(guān)重要的。目前最常見(jiàn)的方法是采用玻璃電極來(lái)測(cè)定pH值,但是用玻璃電極測(cè)pH值時(shí)常常受到金屬離子(如:鈉、鉀、鋰)的干擾,導(dǎo)致測(cè)定結(jié)果不準(zhǔn)確。與電化學(xué)方法相比,熒光探針的光學(xué)測(cè)量并不存在這些缺點(diǎn)。此外,熒光探針在選擇性、靈敏性、納米級(jí)空間分辨有著非常明顯的優(yōu)勢(shì)。方法:我們以羅丹明B作為探針的基本骨架,連接不同結(jié)構(gòu)的五原或六原雜環(huán)化合物合成目標(biāo)產(chǎn)物,并用熒光分光光度計(jì)對(duì)其熒光性能進(jìn)行研究,同時(shí)也研究了其細(xì)胞毒性和細(xì)胞成像性。結(jié)果:成功合成了熒光分子探針A、B、C、D、E,并通過(guò)1HNMR、13CNMR對(duì)其結(jié)構(gòu)進(jìn)行確認(rèn)。探針D在Britton-Robinson(BR)緩沖溶液中隨著p H由4.26增加到7.26其熒光強(qiáng)度逐漸減弱,溶液顏色也逐漸變淡直至消失。在可逆性實(shí)驗(yàn)中,當(dāng)p H值在4.26和6.98來(lái)回變動(dòng)時(shí),熒光強(qiáng)度也可以恢復(fù)到原來(lái)的值,循環(huán)至少六次。在進(jìn)行抗金屬離子干擾能力實(shí)驗(yàn)時(shí),不管pH值是在7.26還是4.26時(shí)金屬離子對(duì)探針D的識(shí)別都幾乎沒(méi)有影響。而探針D在和PC12、MDA-MB-231細(xì)胞共培養(yǎng)時(shí),低濃度的探針D對(duì)兩種細(xì)胞的損傷都比較小,但令人遺憾的是探針D并沒(méi)有能對(duì)細(xì)胞的溶酶體進(jìn)行染色。結(jié)論:雖然在細(xì)胞成像上探針D沒(méi)有達(dá)到令人滿意的效果,但是其對(duì)質(zhì)子的高選擇性和高靈敏性可以應(yīng)用于弱酸性環(huán)境監(jiān)測(cè)。
[Abstract]:Objective: the pH value plays an important role in various systems, so the detection of pH value is very important.At present, the most common method is to use glass electrode to measure pH value. However, the measurement of pH value by glass electrode is often interfered by metal ions (such as sodium, potassium, lithium), which leads to inaccurate results.Compared with electrochemical method, the optical measurement of fluorescent probe does not have these disadvantages.In addition, the fluorescence probe has obvious advantages in selectivity, sensitivity and nanoscale spatial resolution.Methods: Rhodamine B was used as the basic skeleton of the probe, and the target product was synthesized by connecting the heterocyclic compounds with different structures. The fluorescence properties of the compounds were studied by fluorescence spectrophotometer.The cytotoxicity and imaging properties were also studied.Results: a fluorescent molecular probe, Amonobenzene, was successfully synthesized, and its structure was confirmed by 1HNMR-13CNMR.The fluorescence intensity of probe D decreases with increasing pH from 4.26 to 7.26 in the Britton-Robinson-BR) buffer solution, and the color of the solution gradually decreases and disappears.In the reversibility experiment, the fluorescence intensity can return to the original value when the pH value changes back and forth between 4.26 and 6.98, and the fluorescence intensity can be recycled at least six times.In the experiments of resistance to interference of metal ions, the metal ions have little effect on the recognition of probe D, regardless of whether the pH value is 7.26 or 4.26.However, when the probe D was co-cultured with PC12MDA-MB-231 cells, the low concentration of probe D caused less damage to both cells, but unfortunately, probe D did not stain the lysosomes of the cells.Conclusion: although probe D has not achieved satisfactory results in cellular imaging, its high selectivity and high sensitivity to protons can be applied to weak acid environment monitoring.
【學(xué)位授予單位】：西南醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：O657.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 Xiao-Hang Yang;Shu Li;Zhi-Shu Tang;Xi-Di Yu;Tin Huang;Yong Gao;;A simple,water-soluble,Fe~(3+)-selective fluorescent probe and its application in bioimaging[J];Chinese Chemical Letters;2015年01期

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本文編號(hào)：1763002