發(fā)布時(shí)間：2018-04-17 09:08

  本文選題：整流 + 微米管　； 參考：《湘潭大學(xué)》2017年碩士論文

【摘要】：離子整流(Ion-Current Rectification,ICR)為近年來新興的研究熱點(diǎn),其表現(xiàn)為一個(gè)電壓極性下所記錄到的電流信號(hào)高于另一電壓極性不同但電壓絕對(duì)值相同下所記錄到的電流信號(hào),其電壓-電流曲線類似于固體型整流二極管的電壓-電流曲線�；�于目前對(duì)離子整流的認(rèn)識(shí),管道形狀,表面電荷密度,電滲流,掃速,壓力等對(duì)孔道內(nèi)離子的遷移均起到一定的促進(jìn)與阻礙作用,都會(huì)影響整流的效率。而孔道內(nèi)的離子分布不均勻與整流息息相關(guān),即孔道內(nèi)離子的分布不均等是造成整流的直接原因�，F(xiàn)有的活體電分析化學(xué)方法主要是基于活體原位分析方法和活體在線分析方法,其研究對(duì)象多為具有電化學(xué)活性的物質(zhì)。本文基于聚電解質(zhì)功能化的微米管整流,設(shè)計(jì)和發(fā)展了新型的整流型傳感器,該類型傳感器靈敏度高,專一性強(qiáng),可應(yīng)用于非電化學(xué)活性的物質(zhì)檢測(cè),所以在活體檢測(cè)方面具有獨(dú)特優(yōu)勢(shì)�，F(xiàn)取得的主要研究結(jié)果如下:(1)基于聚咪唑修飾的玻璃管微米管整流研究。本工作將聚咪唑修飾在微米管內(nèi)表面,該種聚合物刷在不同的pH條件下帶有不同量的電荷,通過pH的調(diào)節(jié)可研究表面電荷密度對(duì)整流的影響。以此為基礎(chǔ),該體系詳細(xì)探究了電極的穩(wěn)定性,可逆性,以及在不同掃速下整流的變化情況。并嘗試?yán)�用三層理論模型�?duì)咪唑基修飾的微米管玻璃電極的整流現(xiàn)象做出合理的解釋。隨著微米管整流的理論的完善和發(fā)展,為微米管整流在活體應(yīng)用方面提供了更多的參考。(2)基于聚咪唑修飾的微米管的pH傳感器。在對(duì)聚咪唑修飾的微米管整流進(jìn)行細(xì)致的研究后,發(fā)現(xiàn)其在人工腦脊液的溶液中具有良好的pH響應(yīng)。所以可設(shè)計(jì)一種基于聚咪唑修飾的微米管pH電極用于鼠腦內(nèi)pH的檢測(cè)。但是傳統(tǒng)的循環(huán)伏安法作為整流的檢測(cè)方法來講,時(shí)間分辨率過于低�；�于此,本體系設(shè)計(jì)了一種結(jié)合電壓控制和微米管整流的高時(shí)間分辨率pH電化學(xué)傳感器應(yīng)用于鼠腦內(nèi)時(shí)pH的實(shí)時(shí)在線檢測(cè)。該電極具有良好的穩(wěn)定性,可重復(fù)性及專一性。在鼠腦的pH檢測(cè)中,利用二氧化碳缺氧模型,發(fā)現(xiàn)了該電極具有良好的響應(yīng)靈敏度和高的時(shí)間分辨率,可廣泛用于活體分析的pH檢測(cè)。(3)基于咪唑陽離子功能化微米管整流的ATP(腺嘌呤核苷三磷酸)傳感器。在對(duì)微米玻璃管內(nèi)表面進(jìn)行咪唑陽離子的修飾后,得到了具有整流現(xiàn)象的玻璃微米管電極。使其表面吸附ATP核酸適配體后,可利用咪唑陽離子和ATP核酸適配體的雙識(shí)別元件對(duì)ATP分子進(jìn)行識(shí)別。由于該電極擁有良好的專一性,可將其應(yīng)用于鼠腦內(nèi)ATP濃度的檢測(cè)。將比電極的檢測(cè)區(qū)間與鼠腦內(nèi)ATP濃度進(jìn)行比對(duì),發(fā)現(xiàn)此咪唑陽離子修飾的整流型電極具有合適的檢測(cè)區(qū)間。而且,雙識(shí)別元件可排除腦內(nèi)的生理活性分子和ATP類似物的干擾,對(duì)ATP分子具有極高的專一性。此種新型的整流型傳感器的設(shè)計(jì)很好的解決了復(fù)雜環(huán)境下鼠腦腦脊液中的ATP直接測(cè)定問題。
[Abstract]:Ion (Ion-Current Rectification, ICR rectifier) is a new focus in recent research work, its performance for the current signal of a voltage polarity recorded higher than another voltage of different polarity voltage but the absolute value of the current signal under the same record, the voltage current curve similar to the voltage - solid type rectifier diode the current curve. Current understanding of ion rectification pipeline shape based on the surface charge density, electroosmotic flow, sweep speed, transfer pressure on the pore ion are to promote and hinder the role, will affect the rectification efficiency. And the pore distribution of ions is uneven and rectification are closely related, i.e. the distribution of pore ion inequality is directly caused by the electric current in rectifier. Chemical analysis is the main method of in vivo and in vivo analysis method of online analysis method based on the research object with electricity The chemical activity of substances. The micro polyelectrolyte functionalized tube rectifier based on design and development of a rectifier type sensor model, this type of sensor with high sensitivity and specificity, the material can be used in the detection of non electrochemical activity, so it has unique advantages in vivo detection. The main research results are as follows: (1) poly imidazole modified glass tube rectifier of micron tube. In this work, based on poly imidazole modified in micron tube surface, charge the polymer brushes with different weight in different pH conditions, by adjusting the pH effect of charge density on the surface of the rectifier. On this basis, the detailed inquiry system the electrode stability, reversibility, and the change of rectifier at different scan rates. The rectification phenomenon and try to use three layers of imidazolyl modified theoretical model of micron tube glass electrode to make reasonable Explain. With the development and perfection of the theory of the micron tube rectifier, rectifier tube for micron in vivo applications provide more reference. (2) pH micron poly imidazole modified sensor tube. Based on micron poly imidazole modified tube rectifier is studied in detail and found its good response in pH the solution of artificial cerebrospinal fluid. So the design of a micro poly imidazole modified tube pH electrode for the detection of brain based on pH. But the traditional cyclic voltammetry as the detection method of rectification in terms of time resolution is too low. Based on this, the system set up the real-time online detection meter a combination of voltage control and micrometer the rectification of the high time resolution of pH electrochemical sensor used in the brain of rat pH. The electrode has good stability, reproducibility and specificity. The detection of pH in rat brain, using carbon dioxide hypoxia model, hair Now the electrode has good sensitivity and high temporal resolution, can be widely used for the detection of pH in vivo analysis. (3) imidazole cationic functional micro tube rectifier based on ATP (adenosine three phosphate) sensor. In modification of imidazole cation on micron glass tube surface, the micro glass tube electrode with rectification phenomenon. The adsorption of ATP aptamer, can identify the molecular recognition element ATP with double imidazole cation and ATP aptamer. Because the electrode has good specificity and can be applied to detect the concentration of ATP in rat brain. The electrode detection interval with the concentration of ATP in rat brain were compared, found the rectifier type electrode modified with imidazole cation detection interval appropriate. Moreover, double recognition element can eliminate the brain physiological activity and molecular analogs of ATP interference, The design of the new rectifying sensor is very good for solving the problem of direct determination of ATP in cerebrospinal fluid of rat brain in complex environment. It has high specificity for ATP molecules.

【學(xué)位授予單位】：湘潭大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：O657.1

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本文編號(hào)：1762959