發(fā)布時間：2018-04-16 10:04

  本文選題：熒光碳點 + 熒光探針��； 參考：《蘭州大學(xué)》2017年碩士論文

【摘要】：相較于傳統(tǒng)的熒光染料,熒光納米材料既具備優(yōu)良的光學(xué)穩(wěn)定性和良好的生物相容性,又具有納米材料特有的小尺寸效應(yīng)、介電限域效應(yīng)及宏觀量子隧道效應(yīng)等性質(zhì),在物理、化學(xué)、生物、醫(yī)學(xué)和環(huán)境等領(lǐng)域具有巨大的發(fā)展?jié)摿�。近年�?熒光納米材料的研究受到了人們的高度關(guān)注,已在化學(xué)傳感、生物標(biāo)記、藥物運載、光電設(shè)備和光催化等領(lǐng)域廣泛地應(yīng)用。作為熒光納米材料中重要的新成員,熒光碳點因具有良好的生物相容性、較低的細(xì)胞毒性、突出的光學(xué)性質(zhì)以及良好的化學(xué)穩(wěn)定性而成為研究的熱點。眾多研究者圍繞熒光碳點合成方法的改良、發(fā)光性能的調(diào)控以及發(fā)光機理的探究等方面開展了大量工作。在前人的工作基礎(chǔ)上,本學(xué)位論文研究工作從功能化熒光碳點的設(shè)計合成入手,通過優(yōu)選反應(yīng)原料和制備方法,合成了功能化的熒光碳點,并將其用于檢測環(huán)境中重金屬離子和生物成像等方面,并且對熒光碳點發(fā)射波長的調(diào)控進(jìn)行了探索和研究,提出了一種調(diào)控碳點發(fā)射波長的新方法,將所合成的碳點用于多色細(xì)胞成像研究。本學(xué)位論文共分為4章:第一章:在大量文獻(xiàn)的基礎(chǔ)上,介紹了熒光納米材料的發(fā)展背景,系統(tǒng)闡述了熒光碳點的性質(zhì)、發(fā)光機理、制備方法及其在多個領(lǐng)域的應(yīng)用。第二章:本章利用微波熱解法,以檸檬酸和二乙烯三胺為反應(yīng)物,僅用2分鐘制得一種具有藍(lán)色熒光的水溶性氮摻雜熒光碳點。該氮摻雜碳點在450 nm處發(fā)射強熒光(λex=360 nm),并且測得碳點的相對量子產(chǎn)率為27.7%。所合成的碳點具有優(yōu)異的光學(xué)穩(wěn)定性,當(dāng)Hg2+離子存在時,碳點水溶液能發(fā)生明顯的熒光淬滅;加入谷胱甘肽后,溶液的熒光強度明顯恢復(fù)�；�于此我們設(shè)計了一種“on-off-on”熒光探針用于Hg2+離子和谷胱甘肽的檢測,檢出限分別能達(dá)到23 nM和59 nM。此外,該氮摻雜碳點具有優(yōu)異的抗疲勞性,已成功用作熒光探針來分析檢測水樣中的Hg2+離子,而且還實現(xiàn)了活細(xì)胞中Hg2+離子和谷胱甘肽的可視化檢測。第三章:本章以枸杞子為原料,通過一步水熱法合成了具有激發(fā)依賴性的水溶性熒光碳點。該熒光碳點在未經(jīng)進(jìn)一步表面修飾的情況下的相對熒光量子產(chǎn)率為17.2%,略高于其它以天然產(chǎn)物為原料制備的碳點。此外,碳點的熒光信號明顯受Fe3+的影響,基于此該碳點可以作為Fe3+熒光探針,檢出限可達(dá)21nM,并且實現(xiàn)了在實際樣品和活細(xì)胞中成功檢測Fe3+離子。該熒光碳點還具有激發(fā)依賴性,已成功用于活細(xì)胞的多色細(xì)胞成像。第四章:本章通過二乙烯三胺五乙酸分別與鄰苯二胺、間苯二胺和對苯二胺進(jìn)行水熱反應(yīng)合成了三種水溶性熒光碳點(o-CDs,m-CDs,p-CDs),結(jié)果發(fā)現(xiàn)所得碳點的熒光波長與苯二胺的結(jié)構(gòu)直接相關(guān),鄰、間、對苯二胺所得碳點的熒光顏色依次為藍(lán)、綠和紅色。這說明有可能通過反應(yīng)物的結(jié)構(gòu)來實現(xiàn)碳點熒光顏色的調(diào)控。o-CDs,m-CDs和p-CDs的最佳激發(fā)波長分別是360 nm,445 nm和570 nm,最佳發(fā)射波長分別位于430 nm,510 nm和600 nm。這三種熒光碳點均已成功用于HeLa細(xì)胞的多色細(xì)胞成像,并且m-CDs能夠?qū)崿F(xiàn)細(xì)胞核的熒光標(biāo)記。o-CDs,m-CDs和p-CDs在水溶液中的熒光信號對pH響應(yīng)靈敏,有可能作為pH傳感器用于細(xì)胞內(nèi)pH水平的監(jiān)測。本工作采用簡單原料成功制備了不同發(fā)射顏色的熒光碳點,為實現(xiàn)碳點熒光顏色的調(diào)控提供了一種新思路。
[Abstract]:Compared with traditional fluorescent dyes, fluorescent nano materials possess excellent optical stability and good biocompatibility, but also has the small size effect of nanometer material characteristic, dielectric confinement effect and macroscopic quantum tunneling effect, in physics, chemistry, biology, has great potential for development of medical and environmental fields. In recent years, the research of fluorescent nanomaterials has attracted attention of the people, has been in chemical sensing, biological markers, drug delivery, application of optoelectronic devices and photocatalytic fields widely. As a new member of fluorescent nano materials, fluorescent carbon dots with good biocompatibility and low cytotoxicity. Outstanding optical properties and good chemical stability and has become a research hotspot. Many researchers focus on improvement of fluorescent carbon dots synthesis, luminescent properties and light regulation mechanism research. Has done a lot of work. Based on the previous work, the design and synthesis of research work in this dissertation from the functional fluorescent carbon dots with preparation method by optimizing the reaction material and preparation, functionalization of fluorescent carbon dots were synthesized and used for the detection of heavy metal ions in environmental and biological imaging, and regulation the emission wavelength of fluorescent carbon dots was explored and put forward a new method of controlling carbon emission wavelength, study on color cell imaging are used for the synthesis of carbon. This thesis is divided into 4 chapters: the first chapter: on the basis of a large number of literature, this paper introduces the development background of fluorescent nanomaterials the system describes the properties of fluorescent carbon dots luminescence mechanism, preparation method and its application in many fields. The second chapter: this chapter uses the microwave pyrolysis method, using citric acid and ethylene diamine two three as reactants, only 2 minutes was prepared. A blue fluorescent water-soluble nitrogen doped fluorescent carbon dots. The nitrogen doped carbon emitting strong fluorescence at 450 nm (2 ex=360 nm), and measured the relative quantum yield of carbon point synthesized 27.7%. carbon has excellent optical stability, when the presence of Hg2+ ions, carbon water solution have obvious fluorescence quenching; addition of glutathione, the fluorescence intensity was restored. Based on this we designed a on-off-on fluorescence probe for the detection of Hg2+ ion and glutathione, the detection limit can reach 23 nM and 59 nM. in addition, the nitrogen doped carbon has excellent fatigue resistance, has been successfully used as fluorescent probe to Hg2+ ion analysis of water samples, but also realize the visual detection of Hg2+ ion and glutathione in living cells. The third chapter: in this chapter, Chinese wolfberry as raw material by one-step hydrothermal synthesis has inspired by Lai of water soluble fluorescent carbon. The fluorescent carbon dots without the relative fluorescence quantum yield further surface modification for the case of 17.2%, slightly higher than the other natural products as raw materials for the preparation of carbon. In addition, effects of the fluorescence signal point carbon was significantly affected by Fe3+, this point can be used as carbon Fe3+ fluorescent probe based on the detection limit can reach 21nM, and realize the successful detection of Fe3+ ions in real samples and in living cells. The fluorescent carbon dots has excited dependence, has been successfully used in multi cell imaging of living cells. The fourth chapter two through three ethylene amine five acetic acid and benzene amine two respectively. Two, benzene amine and three kinds of water soluble fluorescent carbon dots synthesized by hydrothermal reaction of benzene amine two (o-CDs, m-CDs, p-CDs), found that directly related to the structure of the carbon dots fluorescence wavelength and two adjacent benzene amine, between the fluorescent color of two benzene amine of the carbon point in order to Blue, green and red. This shows that the structure is possible by the reactants to control.O-CDs carbon fluorescent color, optimum excitation wavelength of m-CDs and p-CDs were 360 nm, 445 nm and 570 nm, the best emission wavelengths were 430 nm, 510 nm and 600 nm. of the three kinds of fluorescent carbon dots have been success for multicolor cell imaging of HeLa cells, and m-CDs can realize the fluorescence labeled.O-CDs nuclei, the fluorescence signal of m-CDs and p-CDs in aqueous solution of pH sensitive response, can be used as a pH sensor for monitoring the intracellular level of pH. The simple material was successfully prepared different emission color fluorescent carbon dots used in this work. Provide a new way for the realization of control point carbon fluorescent color.

【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O657.3

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