發(fā)布時(shí)間：2018-04-15 08:17

  本文選題：連翹脂素 + 人肝微粒體　； 參考：《中國(guó)藥學(xué)雜志》2017年03期

【摘要】：目的建立體外人源肝微粒體孵育體系,應(yīng)用UPLC-Q-TOF-MS法,首次對(duì)連翹脂素在人肝微粒體中的代謝產(chǎn)物進(jìn)行鑒定。方法采用Phenomenex Kinetex C_(18)色譜柱(2.1 mm×100 mm,2.6μm),以0.1%甲酸水-乙腈為流動(dòng)相進(jìn)行梯度洗脫,流速為400μL·min~(-1);進(jìn)樣量5μL。采用正離子模式進(jìn)行檢測(cè),電噴霧離子源,源溫度為550℃,質(zhì)量數(shù)掃描范圍m/z 100~1 000。結(jié)果對(duì)ESI-MS正離子模式下連翹脂素可能的裂解途徑進(jìn)行了推測(cè),同時(shí)鑒定了連翹脂素在肝微粒體中的8個(gè)代謝產(chǎn)物。結(jié)論所建立的連翹脂素在人肝微粒體中的UPLC-Q-TOF-MS測(cè)定法方便快捷,可應(yīng)用于連翹脂素在人肝微粒中的代謝研究,同時(shí)為連翹脂素的藥物代謝動(dòng)力學(xué)提供了依據(jù)。
[Abstract]:Objective to establish a human liver microsomal incubation system in vitro and to identify the metabolites of Forsythia suspensa in human liver microsomes by UPLC-Q-TOF-MS method for the first time.Methods the Phenomenex Kinetex Che _ (18) column was used for the gradient elution with 0.1% formic acid water and acetonitrile as the mobile phase, and the flow rate was 400 渭 L 路min ~ (-1), and the amount of sample was 5 渭 L 路min ~ (-1) 路min ~ (-1) 路mol ~ (-1) 路min ~ (-1) 路L ~ (-1) 路L ~ (-1).The positive ion mode was used to detect the electrospray ion source. The temperature of the source was 550 鈩，

本文編號(hào)：1753286