石油工人職業(yè)緊張致高血壓隊列及相關(guān)分子生物學(xué)研究
發(fā)布時間：2018-07-21 15:21
【摘要】：目的:從隊列研究、分子生物學(xué)方面探索石油工人職業(yè)緊張與高血壓的關(guān)系,為防治石油工人高血壓提供科學(xué)依據(jù)。方法:本研究采用隊列研究的方法,研究基線隊列為本課題組于2013年建立的新疆克拉瑪依石油工人隊列,前瞻性隨訪3年,最終1,233人進(jìn)入隊列。采用職業(yè)緊張量表(OSI-R)和付出-回報失衡問卷(ERI)進(jìn)行流行病學(xué)調(diào)查。采用病例對照研究,選擇DNA合格的樣本進(jìn)行聚合酶鏈?zhǔn)椒磻?yīng)限制性片段長度多態(tài)性(PCR-RFLP)檢測ADD1基因、β2-AR基因,高血壓病例組、對照組各176人。從病例組中篩選出低、高度職業(yè)緊張各3例進(jìn)行450K甲基化芯片測定。結(jié)果:1)本研究的基線隊列共計1,535人,剔除基線時已患高血壓者136人,問卷數(shù)據(jù)無效者22人。2016年隨訪終止時,共失訪144人,最終1,233人進(jìn)入隊列;2)基線隊列石油工人得分與國內(nèi)職業(yè)緊張常模得分相比,除任務(wù)不適外,職業(yè)任務(wù)問卷、個體緊張反應(yīng)問卷和個體應(yīng)對資源問卷及其下屬的子項得分差異均有統(tǒng)計學(xué)意義(P0.05)�；�隊列與隨訪隊列OSI-R得分相比,職業(yè)任務(wù)問卷、個體緊張反應(yīng)問卷及個體應(yīng)對資源問卷得分差異均有統(tǒng)計學(xué)意義(P0.05)。不同性別、族別、工齡、倒班情況、文化程度、工種、婚姻狀況及收入的低、中、高三種職業(yè)緊張程度不同(P0.05),男性、漢族、工齡15年、鉆井工職業(yè)緊張程度較高。隨訪至2016年,共315人在隨訪期間患高血壓,累積發(fā)病率為22.76%。付出回報失衡的工人職業(yè)緊張程度較高(P0.05)。病例組與對照組ERI得分的差異有統(tǒng)計學(xué)意義(P0.05)�？偰懝檀肌⒖偰懠t素和總蛋白在病例組與對照組間差異無統(tǒng)計學(xué)意義(P0.05);3)ADD1基因rs17833172位點病例組與對照組差異有統(tǒng)計學(xué)意義(P0.05)。ADD1基因rs17833171和rs4961兩位點間A-T單體型為高血壓危險因素單體型,G-G單體型為高血壓保護(hù)因素單體型(P0.05)。年齡、工種為鉆井工或采油工、吸煙、rs17833172位點AG+GG基因型、高BMI、高空腹血糖、高乳酸脫氫酶和高尿酸是高血壓的危險因素。rs4961位點與rs1042717位點之間的交互作用是高血壓的保護(hù)因素。職業(yè)緊張與rs17833172位點、付出-回報與rs4961位點之間的交互作用是高血壓產(chǎn)生的保護(hù)因素,付出-回報與rs17833172位點之間的交互作用是導(dǎo)致高血壓產(chǎn)生的危險因素。450K甲基化芯片篩選出高度職業(yè)緊張組共有11,977個差異甲基化位點,低度職業(yè)緊張組共有8,312個差異甲基化位點,不同的染色體所含差異甲基化基因位點不同,ISLANDInd區(qū)域差異甲基化基因最多。結(jié)論:1)克拉瑪依石油工人隊列存在長期的職業(yè)緊張情況,暴露因素相對穩(wěn)定;2)石油工人職業(yè)緊張程度較高,鉆井工緊張程度最高。付出大于回報者職業(yè)緊張程度較高。BMI、甘油三脂、低密度脂蛋白、乳酸脫氫酶等指標(biāo)影響著高血壓的產(chǎn)生;3)ADD1基因rs17833172位點GG基因型是高血壓的易感基因型。ADD1基因rs17833171和rs4961兩位點間A-T單體型為高血壓危險因素單體型,G-G單體型為高血壓保護(hù)因素單體型。年齡大、工種為鉆井工或采油工、吸煙、rs17833172位點AG+GG基因型、高BMI、高空腹血糖、高乳酸脫氫酶和高尿酸能夠?qū)е赂哐獕旱漠a(chǎn)生。個體應(yīng)對資源得分高、攜帶rs4961位點GT+TT基因型是高血壓的保護(hù)因素。各基因位點之間、基因與環(huán)境之間交互作用能夠?qū)е赂哐獕旱漠a(chǎn)生。450K甲基化芯片篩選出高度職業(yè)緊張組存在較多的低甲基化位點,低度職業(yè)緊張組高甲基化位點較多,不同的染色體所含差異甲基化位點不同,ISLANDInd區(qū)域差異甲基化基因最多,高血壓與職業(yè)緊張在全基因組層面上存在甲基化表達(dá)譜的差異。
[Abstract]:Objective: To explore the relationship between occupational stress and hypertension in oil workers from cohort study and molecular biology, and to provide a scientific basis for the prevention and treatment of hypertension in oil workers. Methods: This study was conducted by cohort study, and the baseline cohort was established in 2013 in Karamay, Xinjiang, with a prospective follow-up of 3 years. The final 1233 people entered the cohort. The epidemiological survey was conducted using the Occupational Stress Scale (OSI-R) and the pay return imbalance questionnaire (ERI). A case-control study was used to select the DNA qualified samples for the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) for the detection of the ADD1 gene, the beta 2-AR gene, the hypertension case group and the control group of 176 persons each. From the case group, 3 cases of low and high occupational stress were screened by 450K methylation chip. Results: 1) the baseline cohort of this study was 1535 people, 136 people had been suffering from hypertension at baseline and 22 were followed up at the end of the follow-up of 22.2016, the final 1233 people entered the queue; 2) the baseline queue of oil workers was scored. Compared with the domestic occupational stress norm score, the occupational task questionnaire, the individual stressful response questionnaire, the individual coping resource questionnaire and the sub item scores of the subordinates were statistically significant (P0.05). The baseline cohort was compared with the OSI-R scores in the follow-up cohort, and the occupational task questionnaire, individual stressful response questionnaire and individual coping funds were compared. The difference in the score of the source questionnaire was statistically significant (P0.05). There was a high degree of tension between the three types of occupational stress (P0.05), male, Han, 15 years, and a high degree of tension in the drilling job. 315 people were followed up to 2016, a total of 315 people suffered high blood pressure during the follow-up period. The occupational stress of the workers with the cumulative incidence of 22.76%. was high (P0.05). The difference in ERI scores between the case group and the control group was statistically significant (P0.05). There was no significant difference between the total cholesterol, total bilirubin and total protein in the case group and the control group (P0.05); 3) the ADD1 gene rs17833172 loci group was poor with the control group. The A-T haplotype between rs17833171 and rs4961 two loci of.ADD1 gene was statistically significant (A-T), and G-G haplotype was a type of hypertension protective factor haplotype (P0.05). Age, age, drillers or oil workers, smoking, AG+GG based rs17833172 loci, high BMI, high altitude abdominal blood glucose, high lactate dehydrogenase and high uric acid. The interaction between the.Rs4961 locus and the rs1042717 locus, the risk factor for hypertension, is a protective factor for hypertension. The interaction between occupational stress and rs17833172, the interaction between pay return and rs4961 loci is a protective factor for hypertension, and the interaction between pay and return and rs17833172 sites leads to high blood pressure The risk factors of.450K methylation chips were screened for 11977 different methylation sites in the high occupational stress group. There were 8312 difference methylation sites in the low occupational stress group, different methylation locus in different chromosomes, and the most methylation in the ISLANDInd region. Conclusion: 1) Karamay oil workers' queue There were long-term occupational stress conditions and relatively stable exposure factors; 2) oil workers' occupational stress was higher and the drillers had the highest tension. The higher occupational tension of.BMI, glycerol three fat, low density lipoprotein, lactate dehydrogenase and other indexes affecting the production of hypertension; 3) the rs17833172 locus GG genotype of ADD1 gene. The susceptibility gene type.ADD1 gene rs17833171 and rs4961 two loci between the hypertension and the A-T haplotype are the haplotype of hypertension risk factor, and the G-G haplotype is the haplotype of the hypertension protection factor. The age is large, the type of work is the drilling worker or the oil worker, the smoking, the rs17833172 locus AG+GG genotype, the high BMI, the high altitude abdominal blood sugar, the high lactate dehydrogenase and the high uric acid energy. High blood pressure can lead to high blood pressure. Individual response to resources is high, and rs4961 locus GT+TT genotype is a protective factor for hypertension. The interaction between gene loci, gene and environment can lead to the production of hypertension by.450K methylation chips to screen high occupational stress groups with more hypomethylation sites and low degree occupations. There are more methylation sites in the tension group, different methylation sites in different chromosomes, the most methylation genes in the ISLANDInd region, and the difference in the methylation expression profiles at the whole genome level between hypertension and occupational stress.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R135
本文編號：2135950
[Abstract]:Objective: To explore the relationship between occupational stress and hypertension in oil workers from cohort study and molecular biology, and to provide a scientific basis for the prevention and treatment of hypertension in oil workers. Methods: This study was conducted by cohort study, and the baseline cohort was established in 2013 in Karamay, Xinjiang, with a prospective follow-up of 3 years. The final 1233 people entered the cohort. The epidemiological survey was conducted using the Occupational Stress Scale (OSI-R) and the pay return imbalance questionnaire (ERI). A case-control study was used to select the DNA qualified samples for the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) for the detection of the ADD1 gene, the beta 2-AR gene, the hypertension case group and the control group of 176 persons each. From the case group, 3 cases of low and high occupational stress were screened by 450K methylation chip. Results: 1) the baseline cohort of this study was 1535 people, 136 people had been suffering from hypertension at baseline and 22 were followed up at the end of the follow-up of 22.2016, the final 1233 people entered the queue; 2) the baseline queue of oil workers was scored. Compared with the domestic occupational stress norm score, the occupational task questionnaire, the individual stressful response questionnaire, the individual coping resource questionnaire and the sub item scores of the subordinates were statistically significant (P0.05). The baseline cohort was compared with the OSI-R scores in the follow-up cohort, and the occupational task questionnaire, individual stressful response questionnaire and individual coping funds were compared. The difference in the score of the source questionnaire was statistically significant (P0.05). There was a high degree of tension between the three types of occupational stress (P0.05), male, Han, 15 years, and a high degree of tension in the drilling job. 315 people were followed up to 2016, a total of 315 people suffered high blood pressure during the follow-up period. The occupational stress of the workers with the cumulative incidence of 22.76%. was high (P0.05). The difference in ERI scores between the case group and the control group was statistically significant (P0.05). There was no significant difference between the total cholesterol, total bilirubin and total protein in the case group and the control group (P0.05); 3) the ADD1 gene rs17833172 loci group was poor with the control group. The A-T haplotype between rs17833171 and rs4961 two loci of.ADD1 gene was statistically significant (A-T), and G-G haplotype was a type of hypertension protective factor haplotype (P0.05). Age, age, drillers or oil workers, smoking, AG+GG based rs17833172 loci, high BMI, high altitude abdominal blood glucose, high lactate dehydrogenase and high uric acid. The interaction between the.Rs4961 locus and the rs1042717 locus, the risk factor for hypertension, is a protective factor for hypertension. The interaction between occupational stress and rs17833172, the interaction between pay return and rs4961 loci is a protective factor for hypertension, and the interaction between pay and return and rs17833172 sites leads to high blood pressure The risk factors of.450K methylation chips were screened for 11977 different methylation sites in the high occupational stress group. There were 8312 difference methylation sites in the low occupational stress group, different methylation locus in different chromosomes, and the most methylation in the ISLANDInd region. Conclusion: 1) Karamay oil workers' queue There were long-term occupational stress conditions and relatively stable exposure factors; 2) oil workers' occupational stress was higher and the drillers had the highest tension. The higher occupational tension of.BMI, glycerol three fat, low density lipoprotein, lactate dehydrogenase and other indexes affecting the production of hypertension; 3) the rs17833172 locus GG genotype of ADD1 gene. The susceptibility gene type.ADD1 gene rs17833171 and rs4961 two loci between the hypertension and the A-T haplotype are the haplotype of hypertension risk factor, and the G-G haplotype is the haplotype of the hypertension protection factor. The age is large, the type of work is the drilling worker or the oil worker, the smoking, the rs17833172 locus AG+GG genotype, the high BMI, the high altitude abdominal blood sugar, the high lactate dehydrogenase and the high uric acid energy. High blood pressure can lead to high blood pressure. Individual response to resources is high, and rs4961 locus GT+TT genotype is a protective factor for hypertension. The interaction between gene loci, gene and environment can lead to the production of hypertension by.450K methylation chips to screen high occupational stress groups with more hypomethylation sites and low degree occupations. There are more methylation sites in the tension group, different methylation sites in different chromosomes, the most methylation genes in the ISLANDInd region, and the difference in the methylation expression profiles at the whole genome level between hypertension and occupational stress.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R135
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