云南某高校南亞留學(xué)生、國內(nèi)學(xué)生心理因素與消化道優(yōu)勢菌群相關(guān)性研究
發(fā)布時間：2019-01-11 08:34
【摘要】：目的本研究以在校醫(yī)學(xué)本科生為研究對象,應(yīng)用實時熒光定量PCR法檢測其消化道菌群(口腔菌群和腸道菌群)中優(yōu)勢菌群的數(shù)量,重點了解南亞留學(xué)生與國內(nèi)學(xué)生消化道優(yōu)勢菌群數(shù)量的差異,并研究口腔菌群與腸道菌群數(shù)量間的差異。探討影響消化道優(yōu)勢菌群數(shù)量的因素,分析消化道優(yōu)勢菌群數(shù)量與心理因素的相關(guān)關(guān)系,為提高南亞留學(xué)生在華學(xué)習生活質(zhì)量提供參考,并為全面深入了解消化道優(yōu)勢菌群與其影響因素間的關(guān)系將如何維持消化道微生態(tài)系統(tǒng)平衡提供科學(xué)依據(jù)。方法1、篩選研究對象及調(diào)查取樣:按照嚴格的納入及排除標準,選取2014級醫(yī)學(xué)專業(yè)的50名南亞留學(xué)生及50名國內(nèi)學(xué)生為研究對象。采用抑郁-焦慮-壓力量表、艾森克人格問卷簡式量表、匹茲堡睡眠質(zhì)量指數(shù)量表對研究對象的心理健康狀況進行評價。并采集所有入選者的糞便樣本和口腔拭子樣本,提取基因組DNA,均一化核酸濃度至20μg/m L。2、SYBR GreenⅠ實時熒光定量PC R質(zhì)粒標準品的制備:利用具有各菌群種屬特異性基因的16Sr DNA作為擴增區(qū)設(shè)計引物進行PCR擴增,將擴增的目標片段與質(zhì)粒載體連接轉(zhuǎn)化到大腸桿菌中保存,經(jīng)過測序鑒定后,將質(zhì)粒定量并梯度稀釋作為絕對定量的標準品,建立標準曲線。3、消化道菌群定量檢測:應(yīng)用實時熒光定量PCR法檢測兩組學(xué)生的糞便樣本及口腔拭子樣本中總菌群、擬桿菌屬、雙歧桿菌屬、乳酸桿菌屬、大腸埃希菌、腸球菌屬數(shù)量。4、資料整理與分析:采用SPSS17.0統(tǒng)計學(xué)軟件分析南亞留學(xué)生和國內(nèi)學(xué)生消化道優(yōu)勢菌群數(shù)量間的差異,以及消化道優(yōu)勢菌群數(shù)量與心理因素之間的相關(guān)關(guān)系。結(jié)果1、兩組人群的問卷調(diào)查結(jié)果分析:兩組人群在年齡、性別、體質(zhì)指數(shù)(BMI)、分娩方式、運動習慣、排便習慣等方面差異均無統(tǒng)計學(xué)意義(P0.05),但南亞留學(xué)生組在抑郁、焦慮、壓力3個分量表的得分明顯高于國內(nèi)學(xué)生組(P0.05),差異均具有統(tǒng)計學(xué)意義。2、兩組人群腸道菌群定量分析:與國內(nèi)學(xué)生組比,南亞留學(xué)生組糞便樣本中總菌群、擬桿菌屬、雙歧桿菌屬、乳酸桿菌屬比國內(nèi)學(xué)生組的數(shù)量少(P0.05),大腸埃希菌、腸球菌屬比國內(nèi)學(xué)生組的數(shù)量多(P0.05),差異均具有統(tǒng)計學(xué)意義。3、兩組人群口腔菌群定量分析:與國內(nèi)學(xué)生組相比,南亞留學(xué)生組口腔拭子樣本中擬桿菌屬、雙歧桿菌屬、乳酸桿菌屬比國內(nèi)學(xué)生組的數(shù)量少(P0.05),大腸埃希菌、腸球菌屬比國內(nèi)學(xué)生組的數(shù)量多(P0.05),差異均具有統(tǒng)計學(xué)意義。4、口腔菌群與腸道菌群定量比較分析:口腔菌群(口腔拭子樣本)比腸道菌群(糞便樣本)的總菌群、擬桿菌屬、雙歧桿菌屬、乳酸桿菌屬、大腸埃希菌的數(shù)量均少(P0.05),而腸球菌屬為口腔菌群比腸道菌群數(shù)量多(P0.05),差異均具有統(tǒng)計學(xué)意義。5、兩組人群腸道定植抗力分析:兩組人群腸道定植抗力B/E值除個別外均大于1,南亞留學(xué)生組與國內(nèi)學(xué)生組相比B/E值較低(P0.05),差異具有統(tǒng)計學(xué)意義。6、消化道菌群與心理因素的相關(guān)性分析:糞便樣本中大腸埃希菌數(shù)量與抑郁量表、壓力量表的得分均成正相關(guān)(r=0.243,P0.05;r=0.217,P0.05);口腔拭子樣本中大腸埃希菌數(shù)量與抑郁量表的得分成正相關(guān)(r=0.250,P0.05),差異均具有統(tǒng)計學(xué)意義。結(jié)論1、該校南亞留學(xué)生與國內(nèi)學(xué)生均具有良好的腸道微生態(tài),但國內(nèi)學(xué)生的腸道定植抗力強于南亞留學(xué)生。2、該校南亞留學(xué)生與國內(nèi)學(xué)生在消化道優(yōu)勢菌群數(shù)量上存在一定的差異,表現(xiàn)為南亞留學(xué)生消化道的擬桿菌屬、雙歧桿菌屬、乳酸桿菌屬數(shù)量比國內(nèi)學(xué)生少,而大腸埃希菌、腸球菌屬數(shù)量比國內(nèi)學(xué)生多。3、該校南亞留學(xué)生整體心理狀況良好,但仍有個別學(xué)生的抑郁、焦慮、壓力癥狀嚴重,需要相關(guān)管理人員和心理學(xué)者予以密切關(guān)注,預(yù)防嚴重心理問題的出現(xiàn)。4、消化道菌群中大腸埃希菌的數(shù)量可能與心理因素之間存在一定的相關(guān)性,但具體作用機制有待進一步的研究。
[Abstract]:Objective To study the number of dominant bacteria in the digestive tract bacteria group (oral cavity group and intestinal flora) by real-time fluorescence quantitative PCR method, and to study the difference of the number of dominant bacteria in the digestive tract of the students in South Asia and the domestic students. The differences between the number of intestinal flora and the number of intestinal flora were also studied. The factors influencing the number of the dominant bacteria in the digestive tract were discussed, the relationship between the number of dominant bacteria in the digestive tract and the psychological factors was analyzed, and the reference for the improvement of the quality of life of the students in South Asia was provided. In order to provide a scientific basis for how to maintain the balance of microecosystem in the digestive tract in order to get a thorough understanding of the relationship between the flora of the digestive tract and its influencing factors. Method 1. Filter the study object and investigate the sampling: according to the strict inclusion and exclusion criteria, select 50 South Asian students and 50 national students in the medical profession in 2014 as the study object. The mental health status of the study object was evaluated by the depression-anxiety-pressure scale, the Eysenck Personality Questionnaire (EPQ) and the Pittsburgh Sleep Quality (Pittsburgh) sleep quality. and collecting the stool sample and the oral swab sample of all the selected persons, extracting the genomic DNA, homogenizing the nucleic acid concentration to 20 & mu; g/ mL. 2, and preparing the SYBR Green I real-time fluorescent quantitative PC R plasmid standard product. PCR amplification is carried out by using 16Sr DNA with specific genes of each bacterium group as an amplification region, and the amplified target fragment and the plasmid vector are connected and transformed into E. coli for preservation; after sequencing and identification, the plasmid is quantitatively and gradient diluted to be used as an absolute quantitative standard, Establishment of the standard curve. 3. Quantitative detection of the intestinal flora: The real-time fluorescence quantitative PCR was applied to detect the total bacterial group, the genus Bacteroides, the genus Bifidobacterium, the genus Lactobacillus, the Escherichia coli and the genus Enterococcus in both groups. The statistical software of SPSS17. 0 is used to analyze the difference between the number of the dominant bacteria in the digestive tract of the students in South Asia and the domestic students, as well as the relationship between the number of dominant bacteria in the digestive tract and the psychological factors. Results 1. The results of the questionnaire of the two groups showed no significant difference in age, sex, body mass index (BMI), delivery mode, exercise habit, and defecation habit (P0.05). The scores of three components in pressure were significantly higher than those in the domestic group (P0.05), and the difference was statistically significant. 2. The quantitative analysis of the intestinal flora in the two groups: the total bacteria group, the genus Bacteroides, the genus Bifidobacterium, The number of lactobacillus in the group was less than that in the domestic group (P0.05), and the number of E. coli and Enterococcus was much higher than that in the domestic group (P0.05). The number of Bacteroides, Bifidobacterium, and Lactobacillus in the oral swab samples of the foreign students in South Asia was less than that in the domestic group (P0.05), and the number of E. coli and Enterococcus was much higher than that in the domestic group (P0.05). The difference was statistically significant. The quantitative comparison between the population of the oral cavity and the intestinal flora: the number of the total bacteria group (the oral swab sample) of the oral cavity group (the oral swab sample) is lower than that of the intestinal flora (stool sample), and the number of the Bacteroides, the genus Bifidobacterium, the genus Lactobacillus and the Escherichia coli is less (P0.05). The intestinal colonization resistance of the two groups was higher than that of the two groups (P0.05). The correlation between the number of Escherichia coli and the depression scale and the pressure scale in the stool sample was positively correlated (r = 0.243, P0.05; r = 0.217, P0.05); There was a positive correlation between the number of Escherichia coli and the depression scale in the oral swab samples (r = 0.250, P0.05). Conclusion 1. The foreign students in South Asia and the domestic students have good intestinal microecology, but the intestinal colonization resistance of the domestic students is stronger than that of the students in South Asia. The number of Bacteroides, Bifidobacterium, and Lactobacillus in the digestive tract of the international students in South Asia is less than that of the domestic students, while the number of Escherichia coli and Enterococcus is more than that of the domestic students. 3. The students in South Asia are in good health, but still have the depression and anxiety of individual students. There is a certain correlation between the number of Escherichia coli in the digestive tract and the psychological factors, but the specific mechanism of action is to be further studied.
【學(xué)位授予單位】：大理大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R57
[Abstract]:Objective To study the number of dominant bacteria in the digestive tract bacteria group (oral cavity group and intestinal flora) by real-time fluorescence quantitative PCR method, and to study the difference of the number of dominant bacteria in the digestive tract of the students in South Asia and the domestic students. The differences between the number of intestinal flora and the number of intestinal flora were also studied. The factors influencing the number of the dominant bacteria in the digestive tract were discussed, the relationship between the number of dominant bacteria in the digestive tract and the psychological factors was analyzed, and the reference for the improvement of the quality of life of the students in South Asia was provided. In order to provide a scientific basis for how to maintain the balance of microecosystem in the digestive tract in order to get a thorough understanding of the relationship between the flora of the digestive tract and its influencing factors. Method 1. Filter the study object and investigate the sampling: according to the strict inclusion and exclusion criteria, select 50 South Asian students and 50 national students in the medical profession in 2014 as the study object. The mental health status of the study object was evaluated by the depression-anxiety-pressure scale, the Eysenck Personality Questionnaire (EPQ) and the Pittsburgh Sleep Quality (Pittsburgh) sleep quality. and collecting the stool sample and the oral swab sample of all the selected persons, extracting the genomic DNA, homogenizing the nucleic acid concentration to 20 & mu; g/ mL. 2, and preparing the SYBR Green I real-time fluorescent quantitative PC R plasmid standard product. PCR amplification is carried out by using 16Sr DNA with specific genes of each bacterium group as an amplification region, and the amplified target fragment and the plasmid vector are connected and transformed into E. coli for preservation; after sequencing and identification, the plasmid is quantitatively and gradient diluted to be used as an absolute quantitative standard, Establishment of the standard curve. 3. Quantitative detection of the intestinal flora: The real-time fluorescence quantitative PCR was applied to detect the total bacterial group, the genus Bacteroides, the genus Bifidobacterium, the genus Lactobacillus, the Escherichia coli and the genus Enterococcus in both groups. The statistical software of SPSS17. 0 is used to analyze the difference between the number of the dominant bacteria in the digestive tract of the students in South Asia and the domestic students, as well as the relationship between the number of dominant bacteria in the digestive tract and the psychological factors. Results 1. The results of the questionnaire of the two groups showed no significant difference in age, sex, body mass index (BMI), delivery mode, exercise habit, and defecation habit (P0.05). The scores of three components in pressure were significantly higher than those in the domestic group (P0.05), and the difference was statistically significant. 2. The quantitative analysis of the intestinal flora in the two groups: the total bacteria group, the genus Bacteroides, the genus Bifidobacterium, The number of lactobacillus in the group was less than that in the domestic group (P0.05), and the number of E. coli and Enterococcus was much higher than that in the domestic group (P0.05). The number of Bacteroides, Bifidobacterium, and Lactobacillus in the oral swab samples of the foreign students in South Asia was less than that in the domestic group (P0.05), and the number of E. coli and Enterococcus was much higher than that in the domestic group (P0.05). The difference was statistically significant. The quantitative comparison between the population of the oral cavity and the intestinal flora: the number of the total bacteria group (the oral swab sample) of the oral cavity group (the oral swab sample) is lower than that of the intestinal flora (stool sample), and the number of the Bacteroides, the genus Bifidobacterium, the genus Lactobacillus and the Escherichia coli is less (P0.05). The intestinal colonization resistance of the two groups was higher than that of the two groups (P0.05). The correlation between the number of Escherichia coli and the depression scale and the pressure scale in the stool sample was positively correlated (r = 0.243, P0.05; r = 0.217, P0.05); There was a positive correlation between the number of Escherichia coli and the depression scale in the oral swab samples (r = 0.250, P0.05). Conclusion 1. The foreign students in South Asia and the domestic students have good intestinal microecology, but the intestinal colonization resistance of the domestic students is stronger than that of the students in South Asia. The number of Bacteroides, Bifidobacterium, and Lactobacillus in the digestive tract of the international students in South Asia is less than that of the domestic students, while the number of Escherichia coli and Enterococcus is more than that of the domestic students. 3. The students in South Asia are in good health, but still have the depression and anxiety of individual students. There is a certain correlation between the number of Escherichia coli in the digestive tract and the psychological factors, but the specific mechanism of action is to be further studied.
【學(xué)位授予單位】：大理大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R57
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