發(fā)布時(shí)間：2018-12-30 11:35

【摘要】：目的:研究紅芪多糖對(duì)內(nèi)毒素誘導(dǎo)的大鼠葡萄膜炎TLR-4(Toll樣受體-4)信號(hào)轉(zhuǎn)導(dǎo)通路中下游信號(hào)轉(zhuǎn)導(dǎo)通路元件TRAF6(腫瘤壞死因子受體相關(guān)因子-6)及TRIF(Toll樣受體活化相關(guān)因子)表達(dá)的影響,探討紅芪多糖抑制內(nèi)毒素誘導(dǎo)的大鼠葡萄膜炎可能的作用機(jī)制。方法:實(shí)驗(yàn)選用Wistar大鼠作為研究對(duì)象,采用霍亂弧菌內(nèi)毒素1mg/kg皮下注射的方法,建立大鼠急性前葡萄膜炎動(dòng)物模型。Wistar大鼠160只被隨機(jī)分為4組,每組40只:空白對(duì)照組,EIU模型組,LPS+HPS組(內(nèi)毒素+紅芪多糖組)和HPS組(紅芪多糖組)。LPS+HPS組及空白對(duì)照組大鼠分別在內(nèi)毒素注射前1小時(shí)給予腹腔注射紅芪多糖400 mg/kg及等量磷酸鹽緩沖液。HPS組大鼠只注射紅芪多糖400 mg/kg。每2小時(shí)用裂隙燈觀察大鼠眼前節(jié)炎癥反應(yīng)并評(píng)分。組織病理學(xué)檢查觀察炎癥反應(yīng)水平。實(shí)時(shí)定量PCR檢測(cè)TRAF6及TRIF m RNA的表達(dá)。用蛋白免疫印跡法從蛋白水平檢測(cè)TRAF6及TRIF的表達(dá)。結(jié)果:成功建立急性前葡萄膜炎動(dòng)物模型。LPS+HPS組大鼠眼前節(jié)炎癥反應(yīng)及組織病理學(xué)炎癥表現(xiàn)明顯減輕。實(shí)時(shí)定量PCR結(jié)果顯示紅芪多糖可抑制TRAF6m RNA的表達(dá),抑制其蛋白的降解,紅芪多糖對(duì)TRIF的作用不明顯。結(jié)論:紅芪多糖可以通過(guò)抑制TRAF6核酸的表達(dá)和蛋白的降解緩解內(nèi)毒素誘導(dǎo)的大鼠急性前葡萄膜炎眼內(nèi)炎癥反應(yīng)。紅芪多糖對(duì)EIU模型的干預(yù)途徑主要是通過(guò)阻斷TLR4-My D88依賴途徑而發(fā)揮的作用。
[Abstract]:Objective: to study the effects of hongqi polysaccharide on endotoxin-induced uveitis TLR-4 (Toll like receptor 4) signal transduction pathway element TRAF6 (tumor necrosis factor receptor related factor 6) and TRIF ( The expression of Toll like receptor activation-related factors, To explore the possible mechanism of hongqi polysaccharide inhibiting endotoxin induced uveitis in rats. Methods: Wistar rats were selected as the research object. The animal model of acute anterior uveitis was established by subcutaneous injection of vibrio cholerae endotoxin 1mg/kg. 160 Wistar rats were randomly divided into 4 groups, 40 rats in each group: blank control group. EIU model group, Rats in LPS HPS group (lipopolysaccharide group) and HPS group (Hongqi polysaccharide group). LPS HPS group and blank control group) were given intraperitoneal injection of Hongqi polysaccharide 400 mg/kg and the same amount of phosphate buffer 1 hour before endotoxin injection, respectively. Liquid. Rats in HPS group were injected with polysaccharides of Rhizoma membranaceus for 400 mg/kg.. The inflammatory reaction of rat anterior segment was observed by slit lamp every 2 hours. The level of inflammatory reaction was observed by histopathological examination. The expression of TRAF6 and TRIF m RNA was detected by real-time quantitative PCR. The expression of TRAF6 and TRIF was detected by Western blot at protein level. Results: the inflammatory reaction and histopathological inflammation of anterior uveitis in. LPS HPS group were alleviated obviously. The results of real-time quantitative PCR showed that the polysaccharides of Hongqi could inhibit the expression of TRAF6m RNA and the degradation of its protein, but the effect of polysaccharides on TRIF was not obvious. Conclusion: hongqi polysaccharide can alleviate the intraocular inflammation induced by endotoxin in rats with acute anterior uveitis by inhibiting the expression of TRAF6 nucleic acid and protein degradation. The intervention pathway of APS on EIU model was mainly by blocking the TLR4-My D88 dependent pathway.
【學(xué)位授予單位】：首都醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R773

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