葉黃素對(duì)人眼球筋膜囊成纖維細(xì)胞增殖及凋亡的影響
[Abstract]:Objective to investigate the effect of lutein (Lutein) on proliferation and apoptosis of cultured human ocular fascia fibroblasts. Methods Human Tenon's capsule fibroblasts cultured in vitro were treated with 5~40ug/mlLutein for 48 h. The effect of Lutein on proliferation of fibroblasts was detected by CCK-8 assay. The effect of 5~40ug/mlLutein on the fibroblast of Tenon's capsule was detected by FCM after 24 h, and the effect of Lutein on the apoptosis of fibroblast was detected by Hoechst33258 staining. Results 1.CCK-8 assay showed that different concentrations of Lutein (510 ~ 20g / ml) could significantly inhibit the proliferation of human Tenon capsule fibroblasts cultured in vitro for 48 h (P0. 05), and with the increase of time and concentration, the proliferation of the cells was inhibited significantly compared with the control group (P0. 05). The inhibitory effect of Lutein on cells was enhanced. 2. Flow cytometry showed that 5~40ug/mlLutein could block fibroblasts in G0/G1 phase after 24 hours of treatment, and the number of cells entering S phase decreased with the increase of Lutein concentration. The number of cells stranded in G0/G1 phase was also significantly increased (P0. 05). 3.Hoechst33258 staining showed that 5~40ug/mlLutein could promote the apoptosis of fibroblasts after 24 hours of treatment, and the apoptosis rate increased with the increase of Lutein concentration (P0. 05). Conclusion 1.Lutein can inhibit the proliferation and promote the apoptosis of human Tenon's capsule fibroblasts in vitro. 2. The role of Lutein in anti-scarring of filtering bleb after glaucoma filtration and its mechanism are worthy of further study.
【學(xué)位授予單位】:華中科技大學(xué)
【學(xué)位級(jí)別】:碩士
【學(xué)位授予年份】:2012
【分類號(hào)】:R775
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