【摘要】：目的探討葉黃素（Lutein）對(duì)體外培養(yǎng)的人眼球筋膜囊成纖維細(xì)胞增殖及凋亡的影響。 方法對(duì)體外培養(yǎng)的人Tenon氏囊成纖維細(xì)胞，，用5～40ug/mlLutein作用12、24、48h后采用CCK-8法檢測(cè)Lutein對(duì)成纖維細(xì)胞增殖的影響；5～40ug/mlLutein對(duì)人Tenon氏囊成纖維細(xì)胞作用24h后采用FCM檢測(cè)Lutein對(duì)成纖維細(xì)胞細(xì)胞周期的影響，用Hoechst33258染色法檢測(cè)Lutein對(duì)成纖維細(xì)胞凋亡的影響。 結(jié)果 1.CCK-8法顯示不同濃度的Lutein（5、10、20、40ug/ml）對(duì)體外培養(yǎng)的人Tenon囊成纖維細(xì)胞作用12、24、48h后，較對(duì)照組可以明顯抑制細(xì)胞增殖（P㩳0.05），且隨著時(shí)間和濃度的增加，Lutein對(duì)細(xì)胞的抑制作用增強(qiáng)； 2.流式細(xì)胞儀檢測(cè)顯示5～40ug/mlLutein對(duì)成纖維細(xì)胞作用24h后能夠?qū)⒊衫w維細(xì)胞阻滯在G0/G1期，進(jìn)入S期的細(xì)胞減少，且隨著Lutein濃度的增加，滯留在G0/G1期的細(xì)胞也明顯增加（P㩳0.05）； 3.Hoechst33258染色法顯示5～40ug/mlLutein對(duì)成纖維細(xì)胞作用24h后有促進(jìn)細(xì)胞凋亡的作用，且隨著Lutein濃度的增加，凋亡率逐漸增加（P㩳0.05）。 結(jié)論 1.Lutein能夠抑制體外培養(yǎng)的人Tenon氏囊成纖維細(xì)胞的增殖以及促進(jìn)其凋亡。 2.關(guān)于Lutein在青光眼濾過(guò)術(shù)后抗濾過(guò)泡瘢痕化的作用及其機(jī)制值得進(jìn)一步研究。
[Abstract]:Objective to investigate the effect of lutein (Lutein) on proliferation and apoptosis of cultured human ocular fascia fibroblasts. Methods Human Tenon's capsule fibroblasts cultured in vitro were treated with 5~40ug/mlLutein for 48 h. The effect of Lutein on proliferation of fibroblasts was detected by CCK-8 assay. The effect of 5~40ug/mlLutein on the fibroblast of Tenon's capsule was detected by FCM after 24 h, and the effect of Lutein on the apoptosis of fibroblast was detected by Hoechst33258 staining. Results 1.CCK-8 assay showed that different concentrations of Lutein (510 ~ 20g / ml) could significantly inhibit the proliferation of human Tenon capsule fibroblasts cultured in vitro for 48 h (P0. 05), and with the increase of time and concentration, the proliferation of the cells was inhibited significantly compared with the control group (P0. 05). The inhibitory effect of Lutein on cells was enhanced. 2. Flow cytometry showed that 5~40ug/mlLutein could block fibroblasts in G0/G1 phase after 24 hours of treatment, and the number of cells entering S phase decreased with the increase of Lutein concentration. The number of cells stranded in G0/G1 phase was also significantly increased (P0. 05). 3.Hoechst33258 staining showed that 5~40ug/mlLutein could promote the apoptosis of fibroblasts after 24 hours of treatment, and the apoptosis rate increased with the increase of Lutein concentration (P0. 05). Conclusion 1.Lutein can inhibit the proliferation and promote the apoptosis of human Tenon's capsule fibroblasts in vitro. 2. The role of Lutein in anti-scarring of filtering bleb after glaucoma filtration and its mechanism are worthy of further study.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R775

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