本文選題：FMD-VP1-Hetero-6 + 口蹄疫��； 參考：《吉林大學(xué)》2005年博士論文

【摘要】：口蹄疫(Foot and mouth disease,FMD)是一種高度傳染性的疾病,主要發(fā)生于牛、羊、豬等偶蹄類動(dòng)物。口蹄疫病毒(Foot-and-mouth disease virus,FMDV)是引起該病的病原體。由于口蹄疫的高度傳染性和對(duì)農(nóng)業(yè)經(jīng)濟(jì)的重大影響,國(guó)際獸醫(yī)局(OIE)將其列為A 類傳染病。 FMDV 衣殼蛋白VP1 是最主要的抗原蛋白,VP1 刺激機(jī)體產(chǎn)生的抗體具有中和保護(hù)作用,細(xì)胞免疫對(duì)抗體的產(chǎn)生有輔助作用。本論文在設(shè)計(jì)抗FMDV 重組蛋白疫苗選擇VP1 蛋白為靶點(diǎn),具體選擇O 型FMDV VP1的B 細(xì)胞表位(140-160aa)和T 細(xì)胞表位(20-40aa)和(200-213aa)經(jīng)重復(fù)多次組成FMD-VP1-Hetero6 重組蛋白。 我們用基因工程的辦法合成并且純化獲得了FMD-VP1-Hetero6 重組蛋白,功能實(shí)驗(yàn)的結(jié)果顯示:FMD-VP1-Hetero6 刺激豚鼠產(chǎn)生了高滴度的保護(hù)性中和抗體,在乳鼠中和保護(hù)實(shí)驗(yàn)中保護(hù)效價(jià)達(dá)到1:1024。 本研究的主要?jiǎng)?chuàng)新點(diǎn)在于:基于前人的研究基礎(chǔ),我們?cè)O(shè)計(jì)了B 細(xì)胞表位和兩種不同T 細(xì)胞表位交叉多次重復(fù)的疫苗結(jié)構(gòu),該結(jié)構(gòu)未見(jiàn)文獻(xiàn)報(bào)道,為我們首次提出,功能實(shí)驗(yàn)證明此重組蛋白免疫豚鼠的血清在乳鼠中和實(shí)驗(yàn)中對(duì)同型的FMDV 的感染具有顯著的保護(hù)作用。
[Abstract]:Foot and mouth disease (foot and mouth disease) is a highly infectious disease occurring in cattle, sheep, pigs and other cloven-hoofed animals. Foot-and-mouth disease virus (FMDV) is the pathogen that causes the disease. As a result of the highly contagious nature of foot-and-mouth disease and its significant impact on the agricultural economy, The FMDV capsid protein VP1 is the most important antigen protein that stimulates the body to produce the antibody to have the neutralization protection function, the FMDV capsid protein VP1 is the most important antigen protein that stimulates the body to produce the antibody. Cellular immunity assists the production of antibodies. In this paper, the recombinant protein vaccine against FMDV was designed to select VP1 protein as the target, and the B cell epitope (140-160 aa) and T cell epitope (20-40 aa) and 200-213aa) of O type FMDV VP1) were selected to constitute FMD-VP1-Hetero6 recombinant protein. The recombinant FMD-VP1-Hetero6 protein was synthesized and purified by genetic engineering. The results of functional experiments showed that: FMD-VP1-Hetero6 stimulated guinea pigs to produce high titers of protective neutralizing antibody, and the protective titer of FMD-VP1-Hetero6 reached 1: 1024 in the suckling mice and in the protective experiments. The main innovations of this study are as follows: based on the previous studies, we designed the vaccine structure of B cell epitopes and two different T cell epitopes, which have not been reported in literature, and are proposed for the first time. The functional tests showed that the serum of guinea pig immunized with the recombinant protein had a significant protective effect on the infection of the same type of FMDV in the neonatal mice and in the experiment.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2005
【分類號(hào)】：R392

【共引文獻(xiàn)】

相關(guān)期刊論文 前2條

1 魯會(huì)軍;金寧一;鄭敏;韓松;霍曉偉;胡博;田明堯;金擴(kuò)世;;Asia 1型口蹄疫病毒多表位基因的設(shè)計(jì)及其在畢赤酵母中的表達(dá)[J];中國(guó)獸醫(yī)科學(xué);2009年01期

2 馬麗娜;劉永生;陳豪泰;孫德惠;張杰;;Asia1型口蹄疫抗原位點(diǎn)研究進(jìn)展[J];中國(guó)人獸共患病學(xué)報(bào);2008年12期

相關(guān)會(huì)議論文 前1條

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相關(guān)博士學(xué)位論文 前3條

1 冉波;O型口蹄疫病毒VP1表位重組蛋白疫苗的功能研究[D];吉林大學(xué);2006年

2 馬鳴瀟;FMDV分子背景分析、病毒拯救及多價(jià)基因工程疫苗研究[D];吉林大學(xué);2007年

3 蘇春霞;口蹄疫基因工程疫苗重組策略的探討[D];南京農(nóng)業(yè)大學(xué);2007年

相關(guān)碩士學(xué)位論文 前1條

1 徐海飛;重組牛亞洲Ⅰ型口蹄疫病毒疫苗的構(gòu)效研究[D];吉林大學(xué);2007年

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本文編號(hào)：2020199