本文關(guān)鍵詞：成都地區(qū)1040份痰液人鼻病毒的分離和分型　出處：《現(xiàn)代預(yù)防醫(yī)學(xué)》2017年18期 　論文類型：期刊論文

  更多相關(guān)文章： 成人HRV感染 流行特征 基因型

【摘要】：目的探討四川省成都地區(qū)發(fā)熱呼吸道住院患者人鼻病毒(HRV)感染的分子流行病學(xué)特征,為本地人鼻病毒感染的防控積累資料。方法于2014-2016年采集成人發(fā)熱呼吸道癥候群住院患者痰液1 040份,提取標(biāo)本核酸,利用逆轉(zhuǎn)錄聚合酶鏈?zhǔn)椒磻?yīng)(RT-PCR)以及巢式PCR(Nested-PCR)擴(kuò)增HRV-5'NCR基因,篩查HRV陽性標(biāo)本,隨機(jī)選取61份陽性標(biāo)本擴(kuò)增VP4/VP2基因,測定核酸序列并進(jìn)行同源性比對和分型。結(jié)果 1 040例標(biāo)本核酸檢出率為10.9%;冬春季檢出率高,隨年齡升高檢出率呈上升趨勢;獲得的61條VP4/VP2序列中,30條為HRV-A型,10條為HRV-B型,21條為HRV-C型;并發(fā)現(xiàn)HRV基因型內(nèi)和型間核酸同源性差異均較大。結(jié)論成都地區(qū)成人發(fā)熱呼吸道癥候群住院患者HRV的感染率高,以A型與C型流行為主,病毒VP4/VP2核酸片段變異較大。
[Abstract]:Objective to investigate the molecular epidemiological characteristics of human rhinovirus (HRV) infection in hospitalized patients with fever and respiratory tract in Chengdu, Sichuan Province. Methods from 2014 to 2016, 1 040 samples of sputum from adult patients with febrile respiratory syndrome were collected and their nucleic acids were extracted. The HRV-5'NCR gene was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) and nested PCR-Nested-PCR. HRV positive samples were screened. VP4/VP2 gene was amplified from 61 positive samples randomly, nucleic acid sequence was determined, homologous alignment and typing were carried out. Results the detection rate of nucleic acid was 10.9% in 1 040 samples. The detection rate was high in winter and spring and increased with age. Among the 61 VP4/VP2 sequences, 30 were HRV-A and 10 were HRV-B. 21 were HRV-C. It was also found that the homology of nucleic acid in and between HRV genotypes was significant. Conclusion the infection rate of HRV in adult patients with febrile respiratory syndrome in Chengdu area is high, mainly in type A and type C. VP4/VP2 nucleic acid fragments of the virus vary greatly.
【作者單位】： 四川大學(xué)華西公共衛(wèi)生學(xué)院(華西第四醫(yī)院);
【基金】："十二五"國家科技重大專項云南及周邊省市區(qū)傳染病病源譜流行規(guī)律研究(2012ZX10004212-002)
【分類號】：R440;R511
【正文快照】： 人鼻病毒(Human Rhinovirus,HRV)屬于小核糖核酸病毒科腸道病毒屬單正鏈RNA病毒,是常見的呼吸道感染病原體。基因全長7 200 bp,編碼4個蛋白和7個非結(jié)構(gòu)蛋白,其中HRV-5‘NCR為人鼻病毒多聚蛋白基因,此片段具有高保守性,高特異度及檢測靈敏度,因此,作為人鼻病毒感染初篩的目標(biāo)基

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