發(fā)布時間：2018-04-17 07:21

  本文選題：色譜-質譜聯(lián)用 + 左金丸�。� 參考：《佳木斯大學》2017年碩士論文

【摘要】：目的 通過應用色譜-質譜聯(lián)用分離檢測技術,對左金丸和香連丸及組成單味藥的揮發(fā)油和生物堿的化學成分進行定性分析;并對左金丸和香連丸的化學成分進行網(wǎng)絡藥理學研究,探索其抗腹瀉、胃潰瘍和潰瘍性結腸炎作用的多成分-多靶點-多通路的作用機制。方法 本研究采用氣相色譜-質譜聯(lián)用和液相色譜-質譜聯(lián)用兩種分離檢測技術,通過對左金丸和香連丸及組成單味藥的揮發(fā)油成分和生物堿類成分提取物的分離檢測,獲得其色譜質譜數(shù)據(jù),并結合數(shù)據(jù)庫和文獻對其化學成分進行鑒定;通過PharmMapper服務平臺、TTD和DrugBank數(shù)據(jù)庫建立多成分-多靶點網(wǎng)絡,采用MAS在線分析系統(tǒng)進行GO富集和KEGG通路注釋分析,然后利用Cytoscape軟件構建左金丸和香連丸抗腹瀉、胃潰瘍和潰瘍性結腸炎的多成分-多靶點-多通路的網(wǎng)絡模型并加以分析。結果 揮發(fā)油部分左金丸共鑒定出63種化合物,香連丸鑒定出76種化合物;單味藥黃連、吳茱萸、木香分別獲得80、78、76種化合物。醇提取物提取部分左金丸和香連丸中鑒定出黃連堿、藥根堿、小檗堿、巴馬汀、去氫木香內酯、木香烴內酯、吳茱萸次堿和吳茱萸堿。網(wǎng)絡藥理學分析預測出左金丸中有30個活性成分作用于10個靶點,關聯(lián)到27條通路;香連丸中有45個活性成分作用于12個靶點,涉及28條通路。結論 本研究從揮發(fā)油和醇提取物兩方面比較全面地闡釋了左金丸和香連丸所含有的化學成分組成,構建出的左金丸和香連丸的抗腹瀉、胃潰瘍和潰瘍性結腸炎的網(wǎng)絡模型體現(xiàn)出藥對多成分、多靶點、多通路的作用機制,為藥對左金丸和香連丸的物質基礎和藥理作用機制的深入研究提供了科學依據(jù)。
[Abstract]:Objective to analyze qualitatively the chemical constituents of volatile oil and alkaloids in Zuojin pills and Xianglian pills by chromatography-mass spectrometry.The chemical constituents of Zuojin Pill and Xianglian Pill were studied by network pharmacology to explore the mechanism of its action against diarrhea, gastric ulcer and ulcerative colitis.Methods Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) were used to separate and detect the volatile oil and alkaloid components of Zuojin pills and Xianglian pills.Obtaining its chromatography-mass spectrometry data and identifying its chemical constituents in combination with databases and literature; establishing multi-component and multi-target networks through the PharmMapper services platform TTD and DrugBank databases,Go enrichment and KEGG path annotation analysis were carried out by MAS online analysis system. Then the network model of Zuojin Pill and Xianglian Pill against diarrhea, gastric ulcer and ulcerative colitis was constructed and analyzed by Cytoscape software.Results A total of 63 compounds were identified in Zuo Jin Pill, 76 compounds were identified in Xianglian Pill, and 80 Rhizoma Coptidis, Evodia rutaecarpa and Euphorbia were obtained respectively.Coptis alkaloids, root alkaloids, berberine, berberine, Bamatin, dehydroxyxycarbenolide, xylvanolactone, rutaecarpine and rutaecarpine were identified by alcohol extract from Zuojin pills and Xianglian pills.Network pharmacological analysis predicted that there were 30 active components acting on 10 targets in Zuojin pills, which were related to 27 pathways, and 45 active components in Xianglian pills acted on 12 targets, involving 28 pathways.Conclusion the chemical constituents of Zuojin Pill and Xianglian Pill were explained comprehensively from the aspects of volatile oil and alcohol extract, and the anti-diarrhea of Zuojin Pill and Xianglian Pill were constructed.The network model of gastric ulcer and ulcerative colitis shows the action mechanism of drug on multi-component, multi-target and multi-pathway, which provides scientific basis for the further study of the material basis and pharmacological mechanism of Zuo Jin Pill and Xianglian Pill.
【學位授予單位】：佳木斯大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R286.0;O657.63

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本文編號：1762626