發(fā)布時(shí)間：2018-04-15 21:33

  本文選題：紫花地丁 + 黃酮碳苷��； 參考：《分析測(cè)試學(xué)報(bào)》2017年01期

【摘要】：采用超高效液相色譜-電噴霧離子源-四極桿飛行時(shí)間串聯(lián)質(zhì)譜(UPLC-ESI-QTOF-MS/MS)方法,以中藥紫花地丁中分離得到的5個(gè)黃酮碳苷化合物為對(duì)照品,研究了黃酮二糖碳苷類化合物在ESI-QTOF-MS/MS質(zhì)譜中的裂解規(guī)律。研究進(jìn)一步證實(shí)了文獻(xiàn)報(bào)道的黃酮二糖碳苷C-6位取代糖基較C-8位糖更易優(yōu)先發(fā)生裂解;但同時(shí)發(fā)現(xiàn)結(jié)構(gòu)中如果含有不穩(wěn)定構(gòu)象的糖基取代時(shí),該規(guī)律不再適用。通過對(duì)照品的質(zhì)譜裂解規(guī)律,并結(jié)合文獻(xiàn)和高分辨質(zhì)譜數(shù)據(jù),成功表征和鑒定了紫花地丁中21個(gè)黃酮二糖碳苷類以及4個(gè)黃酮氧苷類成分。該方法能快速、靈敏、準(zhǔn)確地對(duì)中藥中微量黃酮碳苷類成分的結(jié)構(gòu)進(jìn)行表征和鑒定。
[Abstract]:Using UPLC-ESI-QTOF-MS / MS / MS method, five flavonoids and carbosides isolated from Chinese medicinal herb Artemisia chinensis were used as the reference substance by ultra-high performance liquid chromatography-electrospray ion source-quadrupole time-of-flight mass spectrometry (TTOF-MS).The cleavage of flavonoids in ESI-QTOF-MS/MS mass spectrometry was studied.It is further confirmed that the C-6 substituted glycosyl group of flavonoid disacarboside is more prone to cleavage than C-8 sugar in the literature, but it is found that this rule is no longer applicable if there is an unstable conformation of glycosyl substitution in the structure.21 flavonoids and 4 flavonoids were successfully characterized and identified by mass spectrometric pyrolysis of the reference substance, literature and high resolution mass spectrometry data.This method can be used to characterize and identify the structure of flavonoid glycosides in traditional Chinese medicine quickly, sensitively and accurately.
【作者單位】： 復(fù)旦大學(xué)藥學(xué)院生藥學(xué)教研室;浙江省醫(yī)療器械不良事件監(jiān)測(cè)中心;
【基金】：國(guó)家自然科學(xué)基金面上項(xiàng)目(81073025)
【分類號(hào)】：R284.1;O657.63

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