發(fā)布時間：2018-04-15 21:33

  本文選題：紫花地丁 + 黃酮碳苷��； 參考：《分析測試學報》2017年01期

【摘要】：采用超高效液相色譜-電噴霧離子源-四極桿飛行時間串聯質譜(UPLC-ESI-QTOF-MS/MS)方法,以中藥紫花地丁中分離得到的5個黃酮碳苷化合物為對照品,研究了黃酮二糖碳苷類化合物在ESI-QTOF-MS/MS質譜中的裂解規(guī)律。研究進一步證實了文獻報道的黃酮二糖碳苷C-6位取代糖基較C-8位糖更易優(yōu)先發(fā)生裂解;但同時發(fā)現結構中如果含有不穩(wěn)定構象的糖基取代時,該規(guī)律不再適用。通過對照品的質譜裂解規(guī)律,并結合文獻和高分辨質譜數據,成功表征和鑒定了紫花地丁中21個黃酮二糖碳苷類以及4個黃酮氧苷類成分。該方法能快速、靈敏、準確地對中藥中微量黃酮碳苷類成分的結構進行表征和鑒定。
[Abstract]:Using UPLC-ESI-QTOF-MS / MS / MS method, five flavonoids and carbosides isolated from Chinese medicinal herb Artemisia chinensis were used as the reference substance by ultra-high performance liquid chromatography-electrospray ion source-quadrupole time-of-flight mass spectrometry (TTOF-MS).The cleavage of flavonoids in ESI-QTOF-MS/MS mass spectrometry was studied.It is further confirmed that the C-6 substituted glycosyl group of flavonoid disacarboside is more prone to cleavage than C-8 sugar in the literature, but it is found that this rule is no longer applicable if there is an unstable conformation of glycosyl substitution in the structure.21 flavonoids and 4 flavonoids were successfully characterized and identified by mass spectrometric pyrolysis of the reference substance, literature and high resolution mass spectrometry data.This method can be used to characterize and identify the structure of flavonoid glycosides in traditional Chinese medicine quickly, sensitively and accurately.
【作者單位】： 復旦大學藥學院生藥學教研室;浙江省醫(yī)療器械不良事件監(jiān)測中心;
【基金】：國家自然科學基金面上項目(81073025)
【分類號】：R284.1;O657.63

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本文編號：1755889