將多功能納米平臺的設計合成應用于腫瘤的聯合治療得到研究人員的廣泛關注.本研究通過水熱法制備了形貌均勻的光熱材料碳納米球,表面負載CuO和抗癌藥鹽酸阿霉素(DOX)實現光熱/化學動力/化療聯合治療. CuO通過靜電吸附負載在碳納米球(CNSs)表面,電子在C-2p與Cu-3d之間的躍遷提高了材料的光熱轉換效率. CuO也可以作為化學動力試劑,在腫瘤部位釋放Cu2+并通過Haber-Weiss和類Fenton反應產生羥基自由基誘導腫瘤細胞凋亡. DOX吸附在CuO@CNSs表面,表現出p H響應釋放和近紅外激光刺激響應的釋放效果.研究結果表明, CuO@CNSs-DOX納米平臺在體內外都有很好的抗癌效果,在腫瘤治療方面有很大的應用潛力.

【文章頁數】：25 頁

【文章目錄】：
INTRODUCTION
EXPERIMENTAL SECTION
    Preparation of CNSs and CuO@CNSs
    The release of Cu2+from CuO@CNSs
    Extracellular·OH detection
    Intracellular·OH detection
    Photothermal properties
    Photothermal conversion efficiency
    Live/dead cell staining
    Computational details
    Loading of DOX
    Release behavior of DOX
    Cellular uptake
    Biocompatibility of CuO@CNSs
    In vitro cytotoxicity detection
    Flow cytometry
    Animal experiments
    IR and PA imaging
    Anti-tumor experiment
    Biocompatibility in vivo
    Statistical analysis
RESULTS AND DISCUSSION
    Fabrication and characterization
    The pH-sensitive release of Cu2+and production of·OH
    Photothermal effects of CuO@CNSs
    DOX loading and releasing behavior
    Celluar uptake assays
    In vitro cytotoxicity of CuO@CNSs
    IR and PA bioimaging
    In vivo antitumor efficacy
CONCLUSIONS
    Experimental Chemicals
    Characteristic
    Cell culture and animal models
    Statistical Analysis



本文編號：4009643