發(fā)布時(shí)間：2018-05-27 13:10

  本文選題：腸系膜上靜脈血栓形成 + 低氧誘導(dǎo)因子-1α��； 參考：《華北理工大學(xué)》2017年碩士論文

【摘要】：目的觀察HIF-1-VEGF通路信號(hào)分子在大鼠腸系膜上靜脈血栓形成(Superior mesenteric venous thrombosis,SMVT)腸道組織中的表達(dá),探討該通路在SMVT中的作用。方法1 64只雄性SD大鼠,隨機(jī)分為兩組,假手術(shù)組(Sham組)和實(shí)驗(yàn)組(SMVT組),每組大鼠32只。2 Sham組:僅打開(kāi)腹腔,不阻斷血運(yùn),術(shù)后8h、24h、48h、72h分批處死大鼠;SMVT組:結(jié)扎法建立單純性周圍型SMVT模型,建模后8h、24h、48h、72h分批處死大鼠。3觀察腹腔及腸道大體變化;HE染色觀察大鼠腸道組織病理學(xué)變化;Real-time q PCR檢測(cè)大鼠腸道組織HIF-1α及VEGF m RNA的表達(dá);免疫組織化學(xué)染色及Western blot檢測(cè)大鼠腸道組織HIF-1α及VEGF蛋白的表達(dá)。結(jié)果1大體觀察結(jié)果顯示:Sham組腹腔及腸道未見(jiàn)明顯變化;SMVT組大鼠腹腔出現(xiàn)不同程度及不同性狀積液,靜脈呈不同程度的怒張狀態(tài),腸道呈不同程度的腫脹及血液瘀滯狀態(tài),且均隨時(shí)間延長(zhǎng)而逐漸減輕。2 HE染色結(jié)果顯示:Sham組大鼠腸道組織均無(wú)明顯病理變化;SMVT組大鼠腸道組織均出現(xiàn)不同程度紅細(xì)胞瘀滯,且隨時(shí)間延長(zhǎng)瘀滯程度逐漸減輕。3 Real-time q PCR結(jié)果顯示:SMVT組較Sham組對(duì)應(yīng)各時(shí)間點(diǎn)HIF-1α及VEGF m RNA表達(dá)明顯升高(P0.05),且均呈遞減趨勢(shì)。4免疫組織化學(xué)染色及Western blot結(jié)果顯示:SMVT組較Sham組對(duì)應(yīng)各時(shí)間點(diǎn)HIF-1α及VEGF蛋白表達(dá)明顯升高(P0.05),且均呈先升后降趨勢(shì)。結(jié)論1 SMVT發(fā)病后,HIF-1α表達(dá)增加,并通過(guò)對(duì)其下游靶基因VEGF進(jìn)行調(diào)控,啟動(dòng)血管新生和生長(zhǎng)過(guò)程,對(duì)缺血腸道組織側(cè)枝循環(huán)的建立具有重要意義。2SMVT發(fā)病后,VEGF在HIF-1α的調(diào)控下表達(dá)增加,促進(jìn)血管的新生和生長(zhǎng),對(duì)缺血腸道組織側(cè)枝循環(huán)的建立具有促進(jìn)作用。3 SMVT發(fā)病后,腸道組織缺血缺氧激活HIF-1-VEGF通路,信號(hào)分子HIF-1α及VEGF表達(dá)同步增加,促進(jìn)血管新生和生長(zhǎng),加速側(cè)枝循環(huán)的建立,減輕血液瘀滯及微循環(huán)障礙,減少透壁性腸梗死的發(fā)生。
[Abstract]:Objective to investigate the expression of HIF-1-VEGF signaling molecule in the intestinal tissues of superior mesenteric venous thrombosis (SMBT) in rats, and to explore the role of this pathway in SMVT. Methods Sixty-four male Sprague-Dawley rats were randomly divided into two groups: sham-operated group (Sham group) and experimental group (32 rats in Sham group). The rats were killed in batches at 8 h, 24 h, 48 h and 72 h after operation. The simple peripheral SMVT model was established by ligation. Rats were sacrificed in batches at 8 h, 24 h, 48 h and 72 h to observe the gross changes of abdominal cavity and intestine. The histopathological changes of intestinal tissue were observed by HE staining. Real-time Q PCR was used to detect the expression of HIF-1 偽 and VEGF m RNA in rat intestinal tissue. Immunohistochemical staining and Western blot were used to detect the expression of HIF-1 偽 and VEGF protein in rat intestinal tissue. Results 1 the results of gross observation showed that there were no obvious changes in abdominal cavity and intestine of rats in the control group. In SMVT group, there were different degrees of effusion in abdominal cavity, different degree of irritation in vein, and different degree of swelling and blood stasis in intestinal tract of SMVT group. The results of HE staining showed that there were no obvious pathological changes in intestinal tissue of rats in the control group. All the intestinal tissues of SMVT group had different degrees of erythrocyte stasis. The results showed that the expression of HIF-1 偽 and VEGF m RNA in the Sham group was significantly higher than that in the Sham group, and the expression of HIF-1 偽 and VEGF m RNA in the Sham group was significantly higher than that in the Sham group. The expression of HIF-1 偽 and VEGF m RNA in the Sham group was significantly higher than that in the control group. 4. 4 immunohistochemical staining and Western blot showed that the HIF-1 偽 and VEGF m RNA expression in the Sham group were significantly higher than those in the control group. Compared with Sham group, the expression of HIF-1 偽 and VEGF protein increased significantly at each time point, and the expression of HIF-1 偽 and VEGF protein increased first and then decreased. Conclusion (1) the expression of HIF-1 偽 is increased after the onset of SMVT. By regulating the downstream target gene VEGF and initiating the process of angiogenesis and growth, it is important for the establishment of collateral circulation in ischemic intestinal tissue to increase the expression of SMVT under the control of HIF-1 偽. Promoting angiogenesis and growth, promoting the establishment of collateral circulation of intestinal tissue after 3. 3 SMVT, intestinal ischemia and hypoxia activated HIF-1-VEGF pathway, the expression of signal molecules HIF-1 偽 and VEGF increased synchronously, and promoted angiogenesis and growth. Accelerate the establishment of collateral circulation, reduce blood stasis and microcirculation, reduce the incidence of transmural intestinal infarction.
【學(xué)位授予單位】：華北理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R572.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 李運(yùn)剛;李建軍;周雪梅;;老年血管性癡呆患者血清HIF-1α、VEGF的變化及相關(guān)性研究[J];腦與神經(jīng)疾病雜志;2016年11期

2 廖彬;王麗平;徐沙麗;李中明;李丹;;急性腦梗死患者血清低氧誘導(dǎo)因子-1α與血管內(nèi)皮生長(zhǎng)因子水平及意義[J];中國(guó)老年學(xué)雜志;2016年21期

3 朱國(guó)獻(xiàn);譚毅華;李濤;;HIF-1α及VEGF基因促進(jìn)大鼠缺血組織血管新生的實(shí)驗(yàn)研究[J];深圳中西醫(yī)結(jié)合雜志;2016年18期

4 呂翠巖;張勝容;徐暾海;孫文;賈曉蕾;趙文景;張竹華;蔡朕;鄭桂敏;孟元;趙靜;王暉;陳洋子;劉銅華;;糖痹康對(duì)糖尿病大鼠坐骨神經(jīng)HIF-1α蛋白及HIF-1α mRNA表達(dá)的影響[J];中華中醫(yī)藥雜志;2016年07期

5 張效科;袁有才;鄧，

本文編號(hào)：1942204